Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India

Orf is a viral disease caused by a parapoxvirus, affecting primarily sheep and goats and causes severe economic losses. In this study, a total of 500 sheep from a farm in El-Beheira Governorate, Egypt were examined during spring, 2014. Out of them, 30 sheep showed clinical signs of orf virus infection. The diseased sheep exhibited proliferative lesions on the lips and around the mouth. Polymerase chain reaction (PCR) was used for diagnosis of the disease. For genetic characterization of the Egyptian orf virus, the sequence of a major and highly immunogenic envelope protein gene (B2L gene) was identified and compared with the sequences available from different parts of the world. The virus was detected in 24 out of 30 collected samples (80 %) by PCR. Phylogenetic analyses of the Egyptian orf virus B2L gene showed close genetic relationship with Israel orf viruses those were identified in 2012. In conclusion, this study reports identification and genetic characterization of Egyptian orf virus in sheep in Egypt.

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“…The used treatment regime was effective in recovering the animals. This treatment regime is similar to that reported previously [9].…”

Section: Discussionsupporting

confidence: 90%

“…Polymerase chain reaction (PCR) has been described for the detection of orf virus and proved to be rapid and specific diagnostic method [1,2,7,9]. Rapid diagnosis of the disease followed by rapid implementation of control measures is very important to control the transboundary transmission of the disease.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep

2015

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“…Occasionally, the lesions can be found on the teats and rarely on other organs (Vikoren et al 2008 ). The orf outbreaks were reported across various Indian states, viz., Uttarakhand (Venkatesan et al 2011 ), Assam, Meghalaya (Bora et al 2012 ), Haryana (Kumar et al 2013 ), Uttar Pradesh (Kumar et al 2014 ), Rajasthan (Maan et al 2014 ), Tripura (Venkatesan et al 2018 ), Kashmir (Ahanger et al, 2018 ), Tamil Nadu (Nagarajan et al 2019 ), and Odisha (Sahu et al 2022 ). The ORFV circulating in India had been clustered with Indian and Chinese isolates using phylogenetic analysis previously (Maan et al 2014 ; Sahu et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…The orf outbreaks were reported across various Indian states, viz., Uttarakhand (Venkatesan et al 2011 ), Assam, Meghalaya (Bora et al 2012 ), Haryana (Kumar et al 2013 ), Uttar Pradesh (Kumar et al 2014 ), Rajasthan (Maan et al 2014 ), Tripura (Venkatesan et al 2018 ), Kashmir (Ahanger et al, 2018 ), Tamil Nadu (Nagarajan et al 2019 ), and Odisha (Sahu et al 2022 ). The ORFV circulating in India had been clustered with Indian and Chinese isolates using phylogenetic analysis previously (Maan et al 2014 ; Sahu et al 2022 ). Usually, CE is self-limiting in sheep and goats, but long-standing cases may provide opportunity for bacterial pathogens to invade the lesions leading to complications (Haig and Mercer, 1998 ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology, clinical features, and molecular detection of orf virus in Haryana (India) and its adjoining areas

Kumar

Moudgil

Grakh

et al. 2022

Trop Anim Health Prod

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Orf is an acute, highly contagious, and economically important viral disease of small ruminants. In this study, six orf suspected outbreaks among goats and sheep were investigated from Haryana state and adjoining areas of Rajasthan state during the year 2021. The disease was diagnosed on the basis of clinical signs and molecular identification. The causative agent of the disease, orf virus (ORFV), was confirmed using polymerase chain reaction (PCR) targeting immunodominant envelope antigen (B2L) gene and confirmed by sequencing. The morbidity in goats ranged from 8.75 to 100%, whereas in sheep, it ranged from 0 to 8%. The higher mortality was observed among flocks with mixed infections of orf and peste des petits (PPR) or orf and haemonchosis as compared to other outbreaks. The phylogenetic analysis of sequenced PCR products clustered the current study strains in the same clad with Indian as well as strains from other countries with nucleotide identity more than 99%, signifying a close genetic relationship. The study highlighted the circulation of strains of a single cluster among sheep and goats in Haryana and adjoining areas. Prompt diagnosis of the disease is highly important for facilitating the implementation of control measures to minimize the losses suffered by small and marginal farmers in this region. Further detailed studies are required to delineate the molecular details of ORFV for better understanding the dynamics and molecular epidemiology of strains circulating in the country and for designing the effective vaccines against the disease which are currently lacking in the country.

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“…Low morbidity and mortality might be due to early detection and culling of the affected animals with adaptation of proper biosecurity measures. The present outbreak was recorded in 5 to 7 month age old goats during the month of June i.e late summer (Bouzanch et al, 2013;Maan et al, 2014).…”

Section: Resultsmentioning

confidence: 86%

Clinical and Pathological Features of a typical Caprine Contagious Pustular Dermatitis

Vijayakumar¹,

Srinivasan²,

Ananthi³

2019

Int.J.Curr.Microbiol.App.Sci

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Isolation and molecular characterization of contagious pustular dermatitis virus from Rajasthan, India

Cited by 8 publications

References 17 publications

Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep

Phylogenetic analysis of B2L gene of Egyptian orf virus from naturally infected sheep

Epidemiology, clinical features, and molecular detection of orf virus in Haryana (India) and its adjoining areas

Clinical and Pathological Features of a typical Caprine Contagious Pustular Dermatitis

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