Identification and pathogenicity of<i>Rhizoctonia</i>species isolated from bean and soybean plants in Samsun, Turkey

Erper, İsmail; Özkoç, İbrahim; Karaca, Gürsel

doi:10.1080/03235400903395427

Cited by 35 publications

(25 citation statements)

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“…were detected on different vegetable crops, including bean, cucumber, eggplant, melon, onion, pepper, pumpkin, tomato, watermelon and winter squash worldwide (SNEH et al, 1991;ERPER et al, 2002;2011;MIRMAJLESSI et al, 2012;MISAWA;KUNINAGA, 2010). Karaca et al (2002) isolated 229 isolates belonging to R. solani AG-2-2, AG-4 and AG-5 from roots and rhizosphere soils of the bean plants in Samsun province.…”

Section: Discussionmentioning

confidence: 99%

“…Infected plant tissues were washed under tap water and they were cut into small pieces, placed in 1% NaOCl for 2 min, rinsed twice with sterile-distilled water (SDW) and blotted dry on sterile paper towels. Small pieces of discoloured root tissues were placed on 2% water agar (WA) amended with 3 mL 10% lactic acid in 9-cm-diameter Petri plates (ERPER et al, 2011). After incubation for two days at 25°C in the dark, hyphae were examined under a light microscope (100X), and fungal colonies possessing the general characteristics of Rhizoctonia spp.…”

Section: Collection and Isolation Of Rhizoctoniamentioning

confidence: 99%

“…Two-week-old seedlings were inoculated by gently removing the soil mixture from one side of the stem, placing 15 colonized oat grains in contact with each seedling and covering the inoculum with the soil mixture (ERPER et al, 2011). In control treatments, sterile oat grains were used.…”

Section: Pathogenicity Assaysmentioning

confidence: 99%

“…(AG-A, AG-B and AG-K) had been recovered from plants of bean and their rhizosphere soils in Samsun province (ERPER et al, 2011). Additionally, in Turkey, isolates of AG-1, AG-2 type 1, AG-3, AG-4 and AG-6 of R. solani, and the binucleate Rhizoctonia spp.…”

Section: Introductionmentioning

confidence: 99%

“…R. solani is found on bean, cucumber, eggplant, pepper and tomato, which are important vegetable crops. In addition, R. solani can cause several types of damage, including dampingoff, root rot and web blight on bean (MUYOLO et al, 1993;KARACA et al, 2002;DEMIRCI, 2004;ERPER et al, 2011), damping-off and root rot on cucumber HARATIANA et al, 2013;JAISWAL et al, 2014), brown spot on eggplant (SNEH et al, 1991), damping-off, hypocotyl rot and root rot on pepper (KARACA et al, 2002, VELÁSQUEZ;VICTORIANO, 2007;LOPEZ et al, 2009;TUNCER;EKEN, 2013) and damping-off, foot and root rot on tomato MISAWA;KUNINAGA, 2010;SOLANKI et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and pathogenicity of Rhizoctonia SPP. isolated from vegetable crops grown in greenhouses in Samsun province, Turkey

Erper¹,

Yildirim²

2017

Biosci. J.

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A total of one hundred and five isolates of Rhizoctonia belonging to 7 anastomosis groups (AGs) were obtained from the diseased roots and rhizosphere soils of bean, cucumber, eggplant, pepper and tomato plants grown in greenhouses in Samsun province (Black Sea region, Turkey) during the period 2011-2012. The isolates of Rhizoctonia spp. were examined for their cultural characteristics, anastomosis groups and pathogenicity. Of these, 83.8% were multinucleate Rhizoctonia solani (AG-2, AG-4, AG-5 and AG-6) and 16.2% were binucleate Rhizoctonia (AG-A, AG-E and AG-F). Sixty five of the isolates belonged to AG-4 which was the most frequent group (61.9%) in all greenhouses surveyed. Numbers of the isolates belonging to AG-2 (7.6%), AG-5 (6.7%) and AG-6 (7.6%) were 8, 7 and 8, respectively. Seventeen isolates recovered from greenhouses surveyed were identified as binucleate Rhizoctonia AG-A (1.9%), AG-E (6.7%) and AG-F (7.6%). All isolates of Rhizoctonia spp. tested for growth rates grew at temperatures of 10, 15, 20, 25 and 30°C, whereas they were completely inhibited at 5°C. The results of pathogenicity tests showed that the differences in virulence among isolates of Rhizoctonia spp. were statistically significant (P < 0.001). The tests on bean seedlings showed that the highest disease severity was caused by AG-4 isolates. The disease severity index (DSI) of the R. solani AG-4 isolates ranged from 3.2 to 3.8. In addition, the isolates of three AGs belonging to binucleate Rhizoctonia spp. were generally found to be moderately virulent (DSI 2.0-2.4).

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