Characterization and pathogenicity of Rhizoctonia SPP. isolated from vegetable crops grown in greenhouses in Samsun province, Turkey

Erper, İsmail; Yildirim, Elif

doi:10.14393/bj-v33n2-34580

Cited by 7 publications

(9 citation statements)

References 17 publications

(53 reference statements)

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“…Genetic differences in hypertension and their subgroups have been phylogenetically assessed using PCR-based DNA fingerprint techniques even in the absence of prior knowledge of the sequences [ 34 , 41 , 42 , 43 , 44 ]. Several studies have reported that Rhizoctonia isolates within many AGs have caused diseases in important crops in Turkey to date [ 11 , 45 , 46 , 47 , 48 , 49 ]; however, there are no detailed reports identifying the AGs and their subgroups of Rhizoctonia isolates causing the root rot and wirestem of red cabbage in Turkey. No information is available about the aggressiveness of the red cabbage isolates to red cabbage cultivars commonly cultivated in the country.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Erper

Özer

Kalendar

et al. 2021

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A total of 132 Rhizoctonia isolates were recovered from red cabbage plants with root rot and wirestem symptoms in the province of Samsun (Turkey) between 2018 and 2019. Based on the sequence analysis of the internal transcribed spacer (ITS) region located between the 18S and 28S ribosomal RNA genes and including nuclear staining, these 124 isolates were assigned to multinucleate Rhizoctonia solani, and eight were binucleate Rhizoctonia. The most prevalent anastomosis group (AG) was AG 4 (84%), which was subdivided into AG 4 HG-I (81%) and AG 4 HG-III (3%), followed by AG 5 (10%) and AG-A (6%), respectively. The unweighted pair group method phylogenetic tree resulting from the data of 68 isolates with the inter-PBS amplification DNA profiling method based on interspersed retrotransposon element sequences confirmed the differentiation of AGs with a higher resolution. In the greenhouse experiment with representative isolates (n = 24) from AGs on red cabbage (cv. Rondale), the disease severity index was between 3.33 and 4.0 for multinucleate AG isolates and ranged from 2.5 to 3.17 for AG-A isolates. In the pathogenicity assay of six red cabbage cultivars, one isolate for each AG was tested using a similar method, and all cultivars were susceptible to AG 4 HG-I and AG 4 HG-III isolates. Redriver and Remale were moderately susceptible, while Rescue, Travero, Integro, and Rondale were susceptible to the AG 5 isolate. The results indicate that the most prevalent and aggressive AGs of Rhizoctonia are devastating pathogens to red cabbage, which means that rotation with nonhost-crops for these AGs may be the most effective control strategy. This is the first comprehensive study of Rhizoctonia isolates in red cabbage using a molecular approach to assess genetic diversity using iPBS-amplified DNA profiling.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Erper

Özer

Kalendar

et al. 2021

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“…Previous research reported R. solani and binucleate Rhizoctonia (Ceratobasidium sp.) on tomato plants (Bolkan & Ribeiro, 1985;Eken & Tuncer, 2019;Gondal et al, 2019;Misawa & Kuninaga, 2010;Montealegre et al, 2003;Pourmahdi & Taheri, 2015;Yıldırım & Erper, 2017). Only one report related to soils of the greenhouse tomato growing area had been documented in Samsun province, Türkiye (Yıldırım & Erper, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…The primary objective of this research was to determine which AG occur in the soils of the greenhouse tomato-growing areas of the West Mediterranean Region, Türkiye. Previously, only one report related to the soils of greenhouse tomato-growing areas had been documented in Samsun province, Türkiye (Yıldırım & Erper, 2017). They isolated AG 4 from the rhizosphere region of tomato, but did not determine its subgroups.…”

Section: Introductionmentioning

confidence: 99%

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Isolation, identification, and pathogenicity of Rhizoctonia spp. recovered from soil samples of the greenhouse tomato growing area of the West Mediterranean region, Türkiye

Eken,

Demir,

Uysal

et al. 2024

Journal of Phytopathology

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This study's objective was to identify the anastomosis groups and pathogenicity of Rhizoctonia species from soil samples in tomato production areas of Antalya, Burdur, and Isparta provinces in the West Mediterranean Region of Türkiye during the 2022 season. Soil samples were gathered from tomato plant rhizospheres in 223 different greenhouses, and isolations were done from the soil using the baiting technique. According to the results of morphological characterization and sequence analysis of the internal transcribed spacers of the ribosomal DNA, 47 Rhizoctonia isolates were defined as binucleate and 21 isolates as multinucleate. The collection of binucleate Rhizoctonia (Ceratobasidium sp.) isolates included representatives of Anastomosis Group‐F (61.76%), AG‐A (1.47%), and an unidentified binucleate Rhizoctonia (Ceratobasidium sp.) (5.88%). Multinucleate R. solani included AG 4 HG‐I (26.47%) and AG 4 HG‐II (4.41%). Pathogenicity tests showed statistically significant differences in virulence between binucleate Rhizoctonia (Ceratobasidium sp.) isolates and multinucleate Rhizoctonia isolates. All R. solani AG 4 HG‐II isolates and most of the AG 4 HG‐I isolates were pathogenic, causing severe necrosis symptoms and damping off in tomato seedlings, but binucleate AG‐A and AG‐F isolates were nonpathogenic. This study is the first detailed report about Rhizoctonia species isolated from the soil of tomato plants grown in greenhouse production areas in Türkiye.

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“…2009; Woodhall et al, 2012;Yildirim and Erper, 2017). According to the results of Moliszewska (1999), R. solani AG5 may comprise approximately 60% of the total amount of R. solani strains isolated from sugar beet seedlings in Poland (Moliszewska, 2009).…”

Section: P O S T P R I N Tmentioning

confidence: 99%

<i>Rhizoctonia solani</i> AG5 and its offspring – Morphology and sensitivity to fungicides

et al. 2023

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The objective of these studies was to identify differences and similarities within the progeny of Rhizoctonia solani AG5, which arose from basidiospores produced by the maternal strain ID23. The following characteristics were analyzed: appearance of the mycelium (color, structure, zonation, and presence of sclerotia), growth rate (at 10°C, 20°C, and 30°C), fungicide sensitivity, and hyphal structure.The mycelial color of R. solani AG5 ranged from white/cream to light and dark brown. The structure of the mycelium may be compacted or flattened with visible zoning or fluffy with dark brown sclerotia on the colony surface. Homokaryons and heterokaryons derived from homokaryons were analyzed by constructing a phylogenetic tree using morphological data. Single basidiospore-grown isolates formed a separate subclade, the majority of which were grouped with a maternal isolate; however, heterokaryons derived from them created a separate subclade. In addition, isolates grown in basidiospores germinated at low temperatures created their own group, but with some exceptions. This shows a divergence in the morphological parameters of the subsequent generations and within generations. The optimal temperature for growth was found to be between 20°C and 30°C. The exceptions were strains obtained from basidiospores that germinated at refrigerated temperatures. For these samples, 10°C was found to be the optimal growth temperature. The hyphae of homokaryons were characterized by the presence of branching at an almost right angle and the presence of a septum at the site of constriction of the branch itself. The mean diameter of hyphae ranged 2.93–15.60 μm, depending on the age of hyphae. The fungicidal compounds at a concentration of 10 ppm had no significant effect on the activity of the tested strains, whereas a tenfold increase in the dose reduced the growth ability of the tested isolates. The activity of fungicides containing azoxystrobin, thiuram, or thiophanate-methyl on R. solani resulted in a reduction in the mycelial growth rate only in the case of azoxystrobin and thiuram, and in some cases, it was completely inhibited (thiophanate-methyl).

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Characterization and pathogenicity of Rhizoctonia SPP. isolated from vegetable crops grown in greenhouses in Samsun province, Turkey

Cited by 7 publications

References 17 publications

Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Isolation, identification, and pathogenicity of Rhizoctonia spp. recovered from soil samples of the greenhouse tomato growing area of the West Mediterranean region, Türkiye

<i>Rhizoctonia solani</i> AG5 and its offspring – Morphology and sensitivity to fungicides

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