Identification and nucleotide sequence of the Acinetobacter calcoaceticus encoded trpE gene

Haspel, G.; Hunger, Michael; Schmucker, Robert; Hillen, Wolfgang

doi:10.1007/bf00391756

Cited by 10 publications

(8 citation statements)

References 19 publications

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“…Although the overall similarities are quite high, with identical amino acids ranging between 47 and 64%, it is surprising that the gram-positive B. subtilis trpB gene is more closely related to the gram-negative A. calcoaceticus gene than to that from E. coli. The same observation has been made for the trpE gene (6). The similarities of the trpF genes are much less pronounced, with amino acid identity ranging between 27 and 36%.…”

supporting

confidence: 74%

“…On this basis, the reading frame extends over 1,209 nucleotides to the stop codon TAA at position 2126, encoding a polypeptide of 403 amino acid residues with a calculated molecular weight of 44,2% and striking homologies with other trpB genes. The codon usages (data not shown) showed the expected preference for A or T in the third positions, as described for other trp genes from this organism (6,15). The only exceptions were the Val and Thr codons in trpF and trpB and the Gln codons in trpB.…”

supporting

confidence: 74%

“…Two main signals flanked by two weaker signals were found for both RNAs,'indicating that the transcription start sites of the plasmid-and chromosome-encoded copies are identical. Figure 3B shows (6,15). A potential transcriptional terminator was found between positions 2152 and 2179.…”

mentioning

confidence: 99%

“…calcoaceticus contains seven trp genes, which appear to be organized in three unlinked clusters (22,23). Nucleotide sequences have been reported for the trpGDC cluster (15) and for the unlinked trpE gene (6). A TrpF protein sequence has been described and compared with other TrpF sequences (18).…”

mentioning

confidence: 99%

“…The first ATG codon in this reading frame' was located at position 995 and preceded by a poor potential ribosome-binding sequence. However, the other trp genes from A. calcoaceticus sequenced so far start with an ATG (6,15). Thus, this ATG may also be used as a start codon.…”

mentioning

confidence: 99%

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Molecular cloning, nucleotide sequence, and promoter structure of the Acinetobacter calcoaceticus trpFB operon

Veerabrahma

Hillen

1990

J Bacteriol

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The trpFB operon from Acinetobacter calcoaceticus encoding the phosphoribosyl anthranilate isomerase and the ,I-subunit of tryptophan synthase has been cloned by complementation of a trpB mutation in A. calcoaceticus, identffied by deletion analysis, and sequenced. It encodes potential polypeptides of 214 amino acids with a calculated molecular weight of 23,008 (TrpF) and 403 amino acids with' a molecular weight of 44,296 (TrpB) this organism (10). Three clones in which the trpBJ8 mutation was complemented were obtained, and the respective plasmids carried insertions of about 22, 16, and 9.3 kilobases, respectively. None of these plasmids yielded complementation of the trpA mutation in A. calcoaceticus BD413 trpA23 (22), whereas two of them complemented the trpF mutation in E. coli trpC9830 (F-) (26). A partial restriction map was made of the plasmid with the smaller insert, and the plasmid was subjected to deletion analysis (Fig. 1). In pWH1754, roughly 3.3 kilobases of the DNA from A. calcoaceticus BD4 complemented both trp mutations, whereas further deletions i'n that DNA resulted in loss of complementation. Therefore, this DNA was partially sequenced, and the resulting reading frames were searched for homology to known trpFB genes. This led to the identification of the trp operon on that DNA (Fig. 1)

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supporting

confidence: 74%

supporting

confidence: 74%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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