Identification and molecular characterization of a receptor-like protein kinase gene from Corchorus capsularis

Khao Dawk Mali 105 (KDML105) rice is one of the most important crops of Thailand. It is a challenging task to identify the genes responding to salinity in KDML105 rice. The analysis of the gene co-expression network has been widely performed to prioritize significant genes, in order to select the key genes in a specific condition. In this work, we analyzed the two-state co-expression networks of KDML105 rice under salt-stress and normal grown conditions. The clustering coefficient was applied to both networks and exhibited significantly different structures between the salt-stress state network and the original (normal-grown) network. With higher clustering coefficients, the genes that responded to the salt stress formed a dense cluster. To prioritize and select the genes responding to the salinity, we investigated genes with small partners under normal conditions that were highly expressed and were co-working with many more partners under salt-stress conditions. The results showed that the genes responding to the abiotic stimulus and relating to the generation of the precursor metabolites and energy were the great candidates, as salt tolerant marker genes. In conclusion, in the case of the complexity of the environmental conditions, gaining more information in order to deal with the co-expression network provides better candidates for further analysis.

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Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat

Tripathi¹,

Aggarwal²,

Yadav³

2013

Turk J Biol

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Karnal bunt caused by Tilletia indica is a quarantine disease responsible for qualitative and quantitative losses in wheat. The present study aimed to determine the expression of defense-related genes involved in early stages of infection in response to T. indica by using the differential display reverse transcriptase-PCR method (DDRT-PCR) in susceptible (WL 711) and resistant (HD 29) cultivars of wheat. The DDRT-PCR banding profiles generated by the random primers in combination with oligo-dT primers of resistant and susceptible wheat plants were evaluated at 0 h, 12 h, 24 h, and 48 h after inoculation. Out of 9 sets of primers, 7 sets of primers produced remarkable induced transcripts. The highest up-regulated transcripts were observed in the resistant cultivar (HD 29), whereas the down-regulated transcripts were found in the susceptible (WL 711) cultivar. Several sets of primers were designed from the defenserelated gene sequences available in the GenBank database, and of these, 4 primer sets amplified the desired specific bands. Based on the putative functions, 3 defense-related genes, namely puroindoline protein PINB (AT_KB2), β-1,4-glucanase (AT_KB3), and chitinase (AT_KB7), and 1 housekeeping gene, actin (AT_KB1), were characterized.

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Identification and molecular characterization of a receptor-like protein kinase gene from Corchorus capsularis

Cited by 4 publications

References 35 publications

Identification of six transcripts encoding putative receptor-like protein kinase (RLK) and their expression profiles in Vitis flexuosa infected with pathogens

Identification of six transcripts encoding putative receptor-like protein kinase (RLK) and their expression profiles in Vitis flexuosa infected with pathogens

Two-State Co-Expression Network Analysis to Identify Genes Related to Salt Tolerance in Thai rice

Differential expression analysis of defense-related genes responsive to Tilletia indica infection in wheat

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