Identification and molecular characterization of phytoplasmas and rickettsia pathogens associated with ‘Bunchy Top Symptom’ (BTS) and ‘Papaya Bunchy Top’ (PBT) of papaya in Cuba

Acosta, K.; Zamora, Loidy; Piñol, B.; Fernández, Ana; Chávez, A.; Flores, Gilberto E.; Méndez, Juan Vicente Méndez; Santos, Mariana; Leyva, Neiel; Arocha, Y.

doi:10.1016/j.cropro.2012.11.016

Cited by 17 publications

(17 citation statements)

References 27 publications

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“…We confirmed the previous reported association of the group 16SrI phytoplasma and rickettsia with BTS in Cuba (Acosta et al 2011(Acosta et al , 2013. In addition, our results refer to the first report of E. papayae as a simultaneous vector for the BTS phytoplasma and the rickettsia pathogens.…”

Section: Discussionsupporting

confidence: 81%

“…Random fragment length polymorphism (RFLP) products were visualized in a 3% agarose gel stain with ethidium bromide under UV transilluminator. RFLP patterns of phytoplasma and rickettsia were compared with those phytoplasma controls previously reported (Lee et al 1998) and with those rickettsia controls (Acosta et al 2013), respectively.…”

Section: Rflp Analysis Of Phytoplasma Ampliconmentioning

confidence: 99%

“…Leaf samples were also collected from 10 symptomless papaya plants. DNA was extracted according to Davis et al (1998) with some modifications (Acosta et al 2013). Total DNA of leaf samples from symptomatic and symptomless papayas were used for PCR-based analysis.…”

Section: Sample Collection and Reference Controlsmentioning

confidence: 99%

“…The group 16SrII ("Candidatus Phytoplasma aurantifolia") was associated with BTS in the eastern region of the country (Arocha et al 2006(Arocha et al , 2007, whereas a phytoplasma of the group 16SrI ("Candidatus Phytoplasma asteris", 16SrI-B subgroup) was associated with papaya exhibiting BTS-like symptoms in eastern Cuba (Acosta et al 2011) and BTS symptom in western (Havana province) (Acosta et al 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The phytoplasmas are consistently associated with both BTS and PBT symptoms, and the mixed infections of phytoplasma and rickettsia pathogens can occur in either BTS or PBTaffected papaya fields, which implies new epidemiological constraints for the disease control (Acosta et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Empoasca papayae Oman, 1937 (Hemiptera: Cicadellidae) the simultaneous vector of phytoplasmas and rickettsia associated with “Bunchy Top Symptom” in Cuba

Acosta

Zamora

Piñol

et al. 2017

An Biol

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Ensayos de transmisión mostraron desarrollo de síntomas típicos de BTS en todas las plantas de papaya después de tres meses de post-inoculadas con Empoasca papayae. El fitoplasma identificado en 28 de 45 las plantas inoculadas, presentó, en secuencia de ADN ribosomal 16S, un 99,9% igual al identificado en plantas de campo y con las detectadas previamente en E. papayae en Cuba. Todos son miembros del subgrupo de fitoplasma 16SrI-B. Una rickettsia se identificó en 41 de las 45 plantas inoculadas. Su secuencia parcial del gen sdhA mostró un 100% de identidad con la rickettsia identificada en plantas de campo, E. papayae y las previamente publicadas. Los resultados confirmaron la habilidad de E. papayae para transmitir simultáneamente el fitoplasma y la rickettsia.Palabras clave: Saltahojas, Transmisión, Fitoplasma, Rickettsia. AbstractEmpoasca papayae Oman, 1937 (Hemiptera: Cicadellidae) vector simultáneo de phytoplasmas y rickettsia asociadas a "Bunchy Top Symptom" en CubaTransmission trials showed typical BTS symptoms developed in all papaya plants after three months post-inoculated with Empoasca papayae. The BTS phytoplasma was identified in 28 out of 45 inoculated papayas, whose partial 16S rDNA sequence was 99.9% identical to those of the phytoplasma identified in the field-collected papayas, and that previously detected in E. papayae in Cuba. These are all members of the phytoplasma subgroup 16SrI-B. A rickettsia was identified in 41 out of 45 inoculated papayas. It showed a 100% of identity of its partial sdhA sequence to those from the field-collected papayas, E. papayae and the rickettsia previously identified. Results confirmed the ability of E. papayae to simultaneously transmit the BTS phytoplasma and the rickettsia pathogen.

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Section: Discussionsupporting

confidence: 81%

Section: Rflp Analysis Of Phytoplasma Ampliconmentioning

confidence: 99%