Diversity of Phytoplasmas in Cuba, Their Geographic Isolation and Potential Development of Management Strategies

Acosta, K.; Pantoja, Madelaine Quiñones; Pérez‐López, Edel

doi:10.1007/978-3-030-29650-6_4

Cited by 2 publications

(3 citation statements)

References 50 publications

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“…Clonal cleaning is a technique widely used to propagate plants infected by viruses (Cronauer & Krikorian, 1985;Kartha & Gambog, 1975), bacteria (Meng et al, 2019), fungi (Cronauer & Krikorian, 1985) and phytoplasma (Accosta, Pantoja, & Perez-Lopez, 2020;Kogej et al, 2020;Tiwari, Tripathi, Lal, Sharma, & Chiemsombat, 2011). Meristem culture, chemotherapy and thermotherapy are among the techniques mostly used to remove pathogens (clonal cleaning).…”

Section: Introductionmentioning

confidence: 99%

“…Meristem culture, chemotherapy and thermotherapy are among the techniques mostly used to remove pathogens (clonal cleaning). However, using antibiotics to control the aforementioned pathogens is difficult because they often translocate through the phloem, and it prevents the pathogens from being fully eliminated; moreover, this process does not protect plants for long periods-of-time (Accosta et al, 2020). Thus, the association between thermotherapy and meristem culture is widely used as strategy.…”

Section: Introductionmentioning

confidence: 99%

“…Assumingly, lack of viral particles in meristematic tissues is related to the accelerated growth and development of these tissues, as well as to vascular disconnection, high protein synthesis level and viral particles' inactivation (Mellor & Stace-Smith, 1977;Kassanis & Varma, 1967). This technique is widely used for clonal cleaning in plants infected by viruses (Kartha & Gambog, 1975;Krikorian and Cronauer, 1984), fungi (Pescador & Koller, 1992;Krikorian & Cronauer, 1984), bacteria (Meng et al, 2019) and phytoplasma (Kogej et al 2020;Accosta et al, 2020;Tiwari et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effects of thermotherapy and meristem culture techniques on macro and micronutrients content in elephant grass cultivars

Karasawa

Tavares

Pinto

et al. 2023

Agron. Sci. Biotechnol.

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Elephant grass is a tropical forage crop highly used in dairy cattle production, in Brazil. It has been getting special attention, because of its bioenergy potential, medicinal properties, and bioremediation profile. The aim of the present study is to investigate the effects of thermotherapy based on clonal cleaning methods and meristem culture on the mineral content of elephant grass (Cenchrus purpureus (Schumach.) Morrone). Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the following methods: thermotherapy (T) combined to meristem culture (MC), meristem culture and mature stems (control). The experiment assessed the mineral contents of phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulfur (S) at three cuttings, which were performed every 60 days, for 180 days. There was lack of effects from these methods on the mineral content of approximately 66% of the carried out assessments, standing out unanimity for all cultivars, methods, and most of the cuts. T+MC was the only method showing positive effect on P and Ca content, in all cuts, in the cultivars Taiwan A-147 and Pioneiro, respectively. There was clear negative effect of cleaning methods on P and Ca content, in all cuts, for cultivar Mineiro, and on Mg, for cultivar Taiwan A-147. These results, along with the positive effects observed in vegetative and nutritional parameters shown in other articles published in this Journal, show that the clonal cleaning methods are strongly recommended for cultivars with more than 15 years of ripe stem propagation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of thermotherapy and meristem culture techniques on macro and micronutrients content in elephant grass cultivars

Karasawa

Tavares

Pinto

et al. 2023

Agron. Sci. Biotechnol.

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Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars

Karasawa¹,

Botega²,

Pinto³

et al. 2023

Agron. Sci. Biotechnol.

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Elephant grass plays important role in dairy cattle production and has received special attention due to its potential in bioenergy and phytoremediation, as well as to its medicinal properties. The aim was investigating the effects of thermotherapy and meristem culture on elephant grass (Cenchrus purpureus (Schumach.) Morrone) forage production and nutritional value. Cultivars “Mineiro”, “Taiwan A-147” and “Pioneiro” were subjected to the methods: thermotherapy plus meristem culture, meristem culture and mature stems (control). The experiment assessed the tiller number (TN), tiller height (TH), number of leaves/tiller (NLT), leaf/stem ratio (LSR), crude protein (CP), acid detergent fiber (ADF), and neutral detergent fiber (NDF), at three cuts performed every 60 days for 180 days. It was observed beneficial influence on the evaluated traits, which indicated how cleaning methods work. Cultivar “Mineiro”, with more than 20 years of use, was more sensitive to the cleaning methods showing than cultivars “Taiwan A-147” (15 years of use) and “Pioneiro” (less than 10 years). It was observed that methods have affected the NT, TH, SDM, LSR, LDM, CP, and NDF. And, that the cultivars genotype effect in a different way the NT, TH, NLT, SDM, LSR, and CP. Overall, Cleaning methods produced an increase in the evaluated parameters over 100% for the cultivars Mineiro and Taiwan when compared with the traditional method and presented low or negative effect for Pioneiro cultivar This evidenced that the traditional propagation method affects forage production and quality over generations in vegetative propagated species. Basic biotechnology techniques such as meristem culture associated or not with thermotherapy can restore the productive potential being recommended for old asexually propagated cultivars with more than fifteen years of cultivation.

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Diversity of Phytoplasmas in Cuba, Their Geographic Isolation and Potential Development of Management Strategies

Cited by 2 publications

References 50 publications

Effects of thermotherapy and meristem culture techniques on macro and micronutrients content in elephant grass cultivars

Effects of thermotherapy and meristem culture techniques on macro and micronutrients content in elephant grass cultivars

Effects of thermotherapy and meristem culture on forage production and nutrition value in elephant grass cultivars

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