Identification and molecular analysis of Ixodid ticks (Acari: Ixodidae) infesting wild boars (Sus scrofa) and tick-borne pathogens at the Meihua mountain of southwestern Fujian, China

Wang, Xin; Sun, Xiaoshuang; Sun, Yankuo; Chen, Kexin; Zhang, Kaiyao; Xu, Weihua; Fan, Kewei; Lin, Wei-Ming; Chen, Tengteng; Lin, Xipan; Lin, Kun; Chiu, Hung-chuan; Huang, Cuiqin

doi:10.1016/j.vprsr.2020.100492

Cited by 6 publications

(6 citation statements)

References 33 publications

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“…Later the Rickettsia was observed in 63.6% (14/22 pools) of ticks (Haemaphysalis bispinosa, H. flava, H. hystricis, Haemaphysalis longicornis, Dermacentor atrosignatus, D. auratus, D. taiwanensis, Dermacentor silvarum) infesting wild boars in Southeast China. However, the study used only partial ompA for the screening for infection [44]. The tick host in our study, D. auratus, has not previously been documented in Taiwan, but our follow-up studies confirmed the continuous existence of the species on the island (unpulished data).…”

Section: Discussionsupporting

confidence: 66%

Seroepidemiological Study of Spotted Fever Group Rickettsiae and Identification of a Putative New Species, Rickesttsia sp. Da-1, in Gongliao, Northeast Taiwan

Yen

Wang

Chang³

et al. 2021

Pathogens

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Tick-borne spotted fever group (SFG) rickettsioses were neglected in Taiwan. The study reported a seroepidemiological survey of SFG rickettsiae in residents in Gongliao District, Northeast Taiwan. Blood samples were examined for antibodies against SFG rickettsiae by enzyme-linked immunosorbent assay and immunofluorescence assay. Risk factors were assessed using logistic regression. Ticks parasitizing dogs were collected within a 2 km radius from the houses of seropositive participants, and PCR was performed to detect possible tick-borne pathogens. Of 1108 participants, 75 (6.8%) had antibodies against SFG rickettsiae. Residents were more likely to be seropositive if they were older than 65 years, recruited by Dr. Enjoy’s Clinic, or resided in Jilin village. A total of 184 ticks including 5 species (Rhipicephalus sanguineus, Rhipicephalus haemaphysaloides, Dermacentor auratus, Haemaphysalis hystricis, Haemaphysalis ornithophila) were collected. Rickettsia spp. were detected in 6.5% (12/184) of ticks. Rickettsia sp. TwKM01 was found in 6 R. sanguineus and 4 R. haemaphysaloides; while Rickettsia sp. TwKM03 was identified in 1 R. sanguineus. Moreover, gene-based pairwise analysis indicated identification of a putative new species, Rickettsia sp. Da-1, in D. auratus. These findings provided evidence of SFG rickettsiae infection in ticks and suggested SFG rickettsiae exposure in the residents.

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Section: Discussionsupporting

confidence: 66%

Seroepidemiological Study of Spotted Fever Group Rickettsiae and Identification of a Putative New Species, Rickesttsia sp. Da-1, in Gongliao, Northeast Taiwan

Yen

Wang

Chang³

et al. 2021

Pathogens

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“…Its main host animals are large wild mammals and livestock, which also invade humans. The peak season of its annual activities is from April to October [ 19 , 26 ]. Interestingly, JMTV was first identified in A. testudinarium , which extended the tick-borne infection spectrum of JMTV and increased the diversity of virus hosts or vectors.…”

Section: Discussionmentioning

confidence: 99%

“…Fujian Province is located in southeastern China, with mountains and hills accounting for > 80% of the total area of the province. It has the highest forest coverage rate of all provinces in China, with a forest area of 76.7 billion km 2 ; as such, it is one of the six major forest areas in China [19]. Its abundant rainfall, sufficient light and superior climatic conditions make it suitable for plant growth and tick survival.…”

Section: Introductionmentioning

confidence: 99%

Identification of Jingmen tick virus (JMTV) in Amblyomma testudinarium from Fujian Province, southeastern China

Zhang

Li²,

Pang

et al. 2022

Parasites Vectors

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Background Jingmen tick virus (JMTV) is a newly discovered tick-borne virus that can cause disease in humans. This virus has been authenticated as being extremely widespread worldwide and as posing a significant threat to public health and safety. Methods We collected 35 ticks belonging to two tick species from wild boars in Nanping, Fujian Province, China. JMTV-specific genes were amplified by qRT-PCR and nested PCR to confirm the presence of this pathogen. Results More than one third of of all ticks collected (11/35) were positive for JMTV. Viral sequences were obtained from three of the JMTV-positive ticks, including the complete genomic sequence from one tick. This was the first time that JMTV was identified in the hard-bodied tick Amblyomma testudinarium. Phylogenetic analysis revealed that JMTV from Fujian Province shared > 90% identity with other isolates derived from China, but was distinct from those reported in France and Cambodia. Conclusions JMTV is characterized by relatively low mutations and has its own local adaptive characteristics in different regions. Our findings provide molecular evidence of the presence of JMTV in an overlooked tick species from an area not unrecognized as being endemic. They also suggest that JMTV occupies a wider geographical distribution than currently believed and is a potential disease vector. Graphical abstract

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“…Indeed, the role of wild boars as carriers of ticks infected by Rickettsia spp. has already been demonstrated on the island of Corsica (France) (Rickettsia slovaca and Rickettsia aeschlimannii) (Defaye et al, 2021), Italy (R. slovaca and Rickettsia raoultii) (Sgroi et al, 2021), China ('Candidatus Rickettsia laoensis') (Wang et al, 2020), Japan (Rickettsia japonica and Rickettsia tamurae) (Motoi et al, 2013;Someya et al, 2015), and Spain (Rickettsia massiliae, Rickettsia slovaca, and Rickettsia raoultii) (Castillo-Contreras et al, 2022). In Brazil, Kmetiuk et al (2019) detected the presence of anti-Rickettsia spp.…”

Section: Recentlymentioning

confidence: 93%

“…Rickettsia sp. has been molecularly detected in ticks collected from wild boars from France (Defaye et al., 2021), Italy (Sgroi et al., 2021), China (Wang et al., 2020), and Spain (Castillo‐Contreras et al., 2022). Piroplasmids (Piroplasmida) were detected in wild boars sampled from the Czech Republic (Hrazdilová et al., 2021) and Japan (Morikawa et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

Santana

Hoppe

Carraro

et al. 2022

Transbounding Emerging Dis

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The present study aimed to investigate, by molecular techniques, the occurrence of Anaplasmataceae, Bartonellaceae, Rickettsiaceae, Mycoplasmataceae, Coxiellaceae, and Babesiidae/Theileriidae agents in blood samples of free-living wild boars (Sus scrofa) and associated ticks in south-eastern Brazil. For this purpose, 67 blood samples and 265 ticks (264 Amblyomma sculptum and one Amblyomma ovale) were analysed.In the screening for Anaplasmataceae agents by a PCR assay based on the 16S rRNA gene, 5.97% blood samples and 50.54% ticks were positive. In the PCR assay for Ehrlichia spp. based on the dsb gene, 9.24% of ticks were positive. Despite the low occurrence, a possible new 16S rRNA genotype of Anaplasma sp. was detected in a wild boar's blood sample. According to phylogenetic analyses based on the 16S rRNA, gltA, and sodB genes and ITS (23S-5S rRNA) intergenic region, it was found that A. sculptum and A. ovale ticks collected from wild boars carry Ehrlichia genotypes phylogenetically associated with Ehrlichia ewingii, Ehrlichia ruminantium, and new Ehrlichia genotypes previously detected in horses, peccaries, and ticks collected from jaguars.In the screening for haemoplasmas by a qPCR based on the 16S rRNA gene, 88.06% of blood samples and 8.69% of ticks were positive. Mycoplasma suis, Mycoplasma parvum, and a possible new haemoplasma genotype were detected in wild boars in southeastern Brazil. In the screening for Bartonella spp. using a nuoG-based qPCR assay, 3.8% of tick samples were positive. Phylogenetic inferences positioned four nuoG and one r gltA Bartonella sequences into the same clade as Bartonella machadoae. No blood or tick samples from wild boars showed to be positive in the qPCR for Coxiella burnetii based on the IS1111 gene. On the other hand, only 1.6% of ticks were positive in the e2808

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Identification and molecular analysis of Ixodid ticks (Acari: Ixodidae) infesting wild boars (Sus scrofa) and tick-borne pathogens at the Meihua mountain of southwestern Fujian, China

Cited by 6 publications

References 33 publications

Seroepidemiological Study of Spotted Fever Group Rickettsiae and Identification of a Putative New Species, Rickesttsia sp. Da-1, in Gongliao, Northeast Taiwan

Seroepidemiological Study of Spotted Fever Group Rickettsiae and Identification of a Putative New Species, Rickesttsia sp. Da-1, in Gongliao, Northeast Taiwan

Identification of Jingmen tick virus (JMTV) in Amblyomma testudinarium from Fujian Province, southeastern China

Molecular detection of vector‐borne agents in wild boars ( Sus scrofa ) and associated ticks from Brazil, with evidence of putative new genotypes of Ehrlichia , Anaplasma , and haemoplasmas

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