Identification and in vitro antimicrobial susceptibility of Staphylococcus species isolated from goat mastitis in the Northeast of Brazil

Silva, Elizabete Rodrigues da; Siqueira, Amanda Pimenta; Martins, Juliana; Ferreira, Wender Paulo Barbosa; Silva, Nivaldo da

doi:10.1016/j.smallrumres.2004.01.001

Cited by 52 publications

(46 citation statements)

References 9 publications

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“…Some CNS species have been The results regarding the antimicrobial activity of reported to be resistant against Penicillin G in caprine honey against the isolated bacteria are presented in [25] and bovine mastitis [26]. This resistance may be Table-2.…”

Section: Hinton Agar According To the National Committee Of Clinical mentioning

confidence: 99%

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Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat's milk

Bourabah¹,

Ayad²,

Hammoudi³

et al. 2014

Vet World

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Aim: The aim of the present study was to determine the susceptibility of subclinical mastitis pathogens isolated from goat's milk and to evaluate the antimicrobial activity of Algerian honey on mastitis causing bacteria. Materials and Methods:The antibacterial activity against the isolated bacteria was evaluated by determining the Minimal Inhibitory Concentration (MIC), using the agar incorporation method. Results:The results showed that both Micrococcus spp. and Klebsiella spp. were susceptible to Streptomycin and tetracycline, while Pseudomonas aeruginosa, E coli, Enterobacter spp., Bacillus spp., and Coagulase Negative Staphyloccoci (CNS) were preferentially susceptible to Streptomycin. However, Streptococcus D was the most resistant to the tested antibiotics whereas Staphylococcus aureus was the most susceptible to all the studied antibiotics. As regards to the antimicrobial activity of honey, the measured values were comprised between 11 and 14%. Conclusion:The results reveal that antimicrobial drugs susceptibility tests in goat subclinical mastitis might be necessary before the treatment. Algerian honey exhibited in vitro antimicrobial activity against different isolated bacteria in goat mastitis.

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Section: Hinton Agar According To the National Committee Of Clinical mentioning

confidence: 99%

“…The authors thank factors including the osmotic effect of honey [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35], Dr. Harrats Ch. (University of Oran, Algeria) for the the presence of hydrogen peroxide, non peroxide English correction.…”

Section: Acknowledgementsmentioning

confidence: 99%

Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat's milk

Bourabah¹,

Ayad²,

Hammoudi³

et al. 2014

Vet World

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“…O uso de antibióticos ou outras drogas de bovinos em pequenos ruminantes, ou até mesmo o uso de produtos em caprinos de produtos indicados para ovinos, constituem um alto risco devido à segurança e eficácia destes produtos em cada espécie serem em grande parte desconhecidos (Mavrogianni et al 2004). Alguns trabalhos realizados no Brasil vêm demonstrando a presença de sensibilidade entre os diferentes patógenos isolados de casos de mastite em caprinos e ovinos (Silva et al 2004, Coutinho et al 2006, Almeida 2009), principalmente para a gentamicina (Mota et al 2000.…”

Section: Estratégias De Controle E Prevençãounclassified

Mastite em pequenos ruminantes no Brasil

2010

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Este artigo objetivou revisar as informações recentes sobre mastite em pequenos ruminantes, abrangendo etiologia, epidemiologia, aspectos de controle e profilaxia. Houve a preocupação em reunir resultados de estudos desenvolvidos no Brasil, uma vez que a mastite tem a interferência de uma série de fatores, como fatores ambientais e outros decorrentes dos sistemas de manejo empregados, condições essas determinantes para etiologia e epidemiologia da enfermidade. A prevalência da mastite em caprinos varia entre 22 e 75%, sendo que os casos de mastite subclínica são os mais frequentes. Existe uma carência de trabalhos voltados para os aspectos epidemiológicos da enfermidade no nosso país. Contudo, observa-se que a mastite vem assumindo importância cada vez maior nos rebanhos voltados para produção de carne, sendo encontrados resultados de pesquisa, principalmente na espécie ovina. A mastite estafilocócica corresponde à maior fração nas infecções intramamárias em pequenos ruminantes. O caráter zoonótico de alguns patógenos, a exemplo do Staphylococcus aureus ressalta a importância da implantação de programas de controle em propriedades leiteiras. Algumas das ferramentas de diagnóstico ainda necessitam de padronização, principalmente para espécie caprina que apresenta uma série de particularidades. Ainda são discutidas as principais estratégias de controle como o manejo de fêmeas e suas crias, os procedimentos de ordenha e a utilização de vacinas.

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“…However, the production system is predominantly extensive or semi--intensive and mostly comprised of small scale producers with major technical limitations. Although staphylococci species have been identiϐied as the most common mastitis--causing agent in small ruminant species in Brazil (Silva et al 2004), knowledge about antimicrobial resistance mechanisms in those bacteria is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

et al. 2012

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Pesq. Vet. Bras. 32(8) The study aimed to determine the antimicrobial resistance patterns and to identify molecular resistance markers in Staphylococcus spp. (n=210) isolated from small ruminant mastitis in Brazil. The antimicrobial resistance patterns were evaluated by the disk diffusion test and by detection of the presence of mecA, blaZ, ermA, ermB, ermC and msrA genes by PCR. The efϐlux pump test was performed using ethidium bromide and bioϐilm production was determined by Congo red agar test along with PCR for detection of the icaD gene. The isolates were most resistant to amoxicillin (50.0%), streptomycin (42.8%), tetracycline (40.4%), lincomycin (39.0%) and erythromycin (33.8%). Pan-susceptibility to all tested drugs was observed in 71 (33.8%) isolates and 41 Staphylococcus isolates were positive for the efϐlux pump. Although phenotypic resistance to oxacillin was observed in 12.8% of the isolates, none harbored the mecA gene. However, 45.7% of the isolates harbored blaZ indicating that beta-lactamase production was the main mechanism associated with staphylococci resistance to beta-lactams in the present study. The other determinants of resistance to antimicrobial agents ermA, ermB, ermC, and msrA were observed in 1.4%, 10.4%, 16.2%, and 0.9% of the isolates, respectively. In addition, the icaD gen was detected in 32.9% of the isolates. Seventy three isolates (54 from goats and 19 from sheep) were negative for all resistance genes tested and 69 isolates presented two or more resistance genes. Association among blaZ, ermA, ermB, ermC and efϐlux pump were observed in 17 isolates, 14 of which originated from goats and three from sheep. The data obtained in this study show the resistance of the isolates to beta-lactamics, which may be associated with the use of antimicrobial drugs without veterinary control.

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Identification and in vitro antimicrobial susceptibility of Staphylococcus species isolated from goat mastitis in the Northeast of Brazil

Cited by 52 publications

References 9 publications

Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat's milk

Antimicrobial activity of Algerian honey on subclinical mastitis pathogens isolated from goat's milk

Mastite em pequenos ruminantes no Brasil

Antimicrobial resistance of Staphylococcus spp. from small ruminant mastitis in Brazil

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