Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene

Alsulami, Muslimah N.; Wakid, Majed H.; Al-Matary, Mohammed; Abdel‐Gaber, Rewaida; Al-Megrin, Wafa Abdullah I.; Bakhraibah, Areej O.; Alanazi, Abdullah D.; Elshabrawy, Hatem A.; El-Kady, Asmaa M

doi:10.2147/idr.s375671

Cited by 2 publications

(1 citation statement)

References 31 publications

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“…To overcome this limitation, it is necessary to obtain a reference genome for F. hepatica that can be used to design more informative markers to reveal the parasite's evolutionary history. Mitochondrial genes have been shown to be informative for phylogenetic studies of F. hepatica due to their high mutation rate (66,67). However, in our study, the use of the COI marker did not allow us to reconstruct phylogenetic relationships within Colombian samples (Figure 3), a situation similar to that found by Chaouadi et al (68) in samples obtained in Algeria.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

García-Corredor¹,

Alvarado²,

Pulido-Medellín³

et al. 2023

Front. Vet. Sci.

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Fasciola hepatica is a zoonotic trematode that affects a wide range of hosts, including cattle, sheep, and goats. The economic impact of the parasite on the cattle industry is significant, with high losses reported worldwide. While its impact on human health was previously underestimated, recent years have seen a rise in fascioliasis cases, leading to increased interest among researchers globally. To characterize the genetic diversity and intraspecific variation of this parasite in South America, specifically in Colombia, we collected 105 adult parasites from cattle bile ducts in seven Colombian departments (Antioquia, Boyacá, Santander, Cauca, Cundinamarca, Nariño, Norte de Santander, and Santander) to assess the parasite’s phenotypic analyses, genetic diversity, and population structure. A computer image analysis system (CIAS) was applied based on standardized morphological measurements. Liver-fluke size was studied by principal component analysis (PCA). DNA sequences were obtained for nuclear markers such as the 28S, β-tubulin 3, ITS1, ITS2, and the mitochondrial marker Cytochrome Oxidase I (COI). Multiple statistical tests were performed, and the parasite’s population structure was analyzed. Maximum Likelihood (ML) phylogenetic reconstructions were carried out using the sequences obtained herein and sequences available in GenBank. Morphological results revealed that all the obtained individuals matched F. hepatica’s morphology. There was no evidence of high genetic diversity, and the absence of genetic structure at the country-level was notable, possibly caused by a demographic expansion of this trematode in Colombia or the low resolution of the molecular markers employed. Future studies are still needed to unveil the genetic population structure of F. hepatica across the country.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

García-Corredor¹,

Alvarado²,

Pulido-Medellín³

et al. 2023

Front. Vet. Sci.

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Molecular identification of Fasciola species based on ribosomal and mitochondrial DNA sequences in Riyadh, Saudi Arabia

Alkahtani,

Almutairi,

Aljasham

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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Fasciola species are the causative agents of liver fluke disease (fasciolosis) which is primarily found in domestic and wild ruminants but also are causal agents of fascioliasis in humans. Few reports for the genotyping of Fasciola species in Saudi Arabia were available. This study aimed to identify the Fasciola species infecting sheep using morphology and molecular tools. For that, livers of 500 imported sheep were slaughtered at abattoirs located in Riyadh (Saudi Arabia), and parasite species were isolated from 28 liver samples and then preserved for subsequent stages in this study. These parasite species were identified as Fasciola hepatica and Fasciolagigantica via morphology and confirmed via the 28S rRNA, ITS-1, COI, and NDI sequences with a close identity for other Fasciola species within the family Fasciolidae. Phylogenetic analysis of the obtained sequences showed that Fasciola isolates from the current study were clustered in one subclade closely related to isolates from Iran, Vietnam, Australia, South Africa, Eastern Europe, India, Egypt, Sudan, Japan, Poland, Spain, Armenia, and Turkey. A combination of data from morphology and molecular analysis could be considered a useful tool for identifying Fasciola species infecting sheep in Saudi Arabia.

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Identification and Genetic Characterization of Fasciola hepatica Isolated from Cattle in Jeddah, Saudi Arabia Based on Sequence Analysis of Mitochondrial (COI) Gene

Cited by 2 publications

References 31 publications

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

Molecular characterization of Fasciola hepatica in endemic regions of Colombia

Molecular identification of Fasciola species based on ribosomal and mitochondrial DNA sequences in Riyadh, Saudi Arabia

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