Identification and functional characterization of a g-type lysozyme gene of Labeo rohita, an Indian major carp species

Mohapatra, Amruta; Parida, Sagarika; Mohanty, Jyotirmaya; Sahoo, P.K.

doi:10.1016/j.dci.2018.11.004

Cited by 19 publications

(17 citation statements)

References 53 publications

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“…However, the activity was less for gram-positive bacteria. Studies have reported that the antimicrobial action occurs through the hydrolysis of b-1,4glycosidic linkage between N-acetylmuramic acid and Nacetylglucosamine alternating the sugar residues in the PGN of bacterial cell walls, thereby inducing bacterial cell lysis (Mohapatra et al, 2019). In this study, rPmlysG damaged the cell wall of V. parahaemolyticus and A. hydrophila, inducing the release of bacterial contents.…”

Section: Discussionmentioning

confidence: 68%

Immune characterization and expression analysis of a goose-type lysozyme gene from Pinctada fucata martensii

Guo

Shen²,

Liang³

et al. 2022

Front. Mar. Sci.

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In the present study, a g-type lysozyme was successfully screened and cloned from Pinctada fucata martensii (designated as PmlysG). The cDNA has a length of 973 bp with an open reading frame (ORF) of 769 bp, encoding a protein of 255 amino acids. The PmlysG transcript was detected in multiple tissues by quantitative real-time PCR (qRT-PCR), with the highest expression being in the hepatopancreas. Additionally, the temporal expression of PmlysG mRNA in the hepatopancreas after in vivo stimulation with pathogen-associated molecular patterns (PAMPs), such as lipopolysaccharide (LPS), peptidoglycan (PGN) and polyinosinic acid (PolyI:C) was detected by qRT-PCR. Although PmlysG responded to all three stimulation modes, it rapidly responded to PGN stimulation. Meanwhile, the recombinant protein of g-type lysozyme of P.f. martensii (rPmlysG) was used for antibacterial function analysis, and the results showed that rPmlysG has antibacterial function against Vibrio parahaemolyticus, Aeromonas hydrophila, and Pseudomonas aeruginosa. Overall, these study results suggest that the identified PmlysG participates in the innate immune responses of P.f. martensii against pathogen infection.

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Section: Discussionmentioning

confidence: 68%

Immune characterization and expression analysis of a goose-type lysozyme gene from Pinctada fucata martensii

Guo

Shen²,

Liang³

et al. 2022

Front. Mar. Sci.

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“…Currently, advances in molecular biology technologies enable researchers to focus on the molecular characterization of the lysozyme gene through sequencing analysis. Consequently, this permits to estimate lysozyme dosage that is assessed by gene expression, and often the production of the enzyme can be further verified through direct or indirect ELISA assays with the use of a specific monoclonal antibody to identify lysozyme (Marsh and Rice, 2010;Mohapatra et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Several types of lysozymes have been described such as c-type (conventional or chicken-type), g-type (goose-type), and invertebrate-type lysozyme (i-type), T4 phage lysozyme, bacterial lysozyme, and plant-type lysozyme (Jiménez-Cantizano et al, 2008;Callewaert and Michiels, 2010;Buonocore et al, 2014). Only g-type and c-type lysozymes have been identified in several fish species (Jiménez-Cantizano et al, 2008;Saurabh and Sahoo, 2008;Buonocore et al, 2014;Mohapatra et al, 2019). In fish, lysozyme genes are expressed in cells of myeloid origin (Hall et al, 2007;Saurabh and Sahoo, 2008), and these are present in mucus, eggs, blood, and tissues with leukocytes (neutrophils, monocytes, and a small amount in macrophages) to defend against microorganisms, such as bacteria (Li et al, 2021;Murray and Fletcher, 1976).…”

Section: Introductionmentioning

confidence: 99%

Lysozyme activity as an indicator of innate immunity of tilapia (Oreochromis niloticus) when challenged with LPS and Streptococcus agalactiae

Biller¹,

Polycarpo²,

Moromizato³

et al. 2021

Revista Brasileira De Zootecnia

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“…However, the strong positive correlation of lysG up-regulation with the viral shedding observed in the present study could also suggest a specific antiviral role of lysozyme in the KHV infection. Increased up-regulation of lysozyme in the serum of mirror carp was measured during KHV infections [ 48 ], and in rohu ( Labeo rohita ) after induction with poly I:C, a dsRNA viral analogue [ 49 ]. Lysozyme plays an important role in the non-specific immune system; its antiviral function has been described in higher vertebrates, yet further studies are required to understand its role during viral infections in teleost fish [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Seroconversion and Skin Mucosal Parameters during Koi Herpesvirus Shedding in Common Carp, Cyprinus carpio

Cano

Mulhearn

Akter

et al. 2020

IJMS

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Seroconversion and the mucosal lysozyme G (lysG), complement 3 (c3), and immunoglobulins M (IgMsec) and Z2 (IgZ2) were measured for up to 900 degree days (DD) in skin swabs from common carp exposed to koi herpesvirus (KHV or CyHV-3) at either a non-permissive temperature (12 °C) or permissive temperatures (17 and 22 °C), and in survivors subjected to temperature increase to 22 °C 500 DD after the initial exposure. The survival rate at 22 °C varied from 100% in fish initially exposed at 12 °C, to 20% at 17 °C and 0% at 22 °C. Viral shedding episodes lasted for up to 29 days (493 DD) for fish clinically infected at 17 °C, and up to 57 days (684 DD) for asymptomatic fish held at 12 °C. Up-regulation of lysG transcripts was measured at 17 and 22 °C. Down-regulation of c3 and IgMsec transcripts was measured independent of the water temperature, followed by up-regulation after the temperature increase coinciding with seroconversion and clearance of KHV from the skin mucus. IgZ2 mRNA showed a negative correlation with IgM transcripts. KHV subversion of the complement system at the mucosal site coupled with poor immunoglobulin secretion during the viral replication might contribute to the long window of viral shedding, thus facilitating viral transmission.

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Identification and functional characterization of a g-type lysozyme gene of Labeo rohita, an Indian major carp species

Cited by 19 publications

References 53 publications

Immune characterization and expression analysis of a goose-type lysozyme gene from Pinctada fucata martensii

Immune characterization and expression analysis of a goose-type lysozyme gene from Pinctada fucata martensii

Lysozyme activity as an indicator of innate immunity of tilapia (Oreochromis niloticus) when challenged with LPS and Streptococcus agalactiae

Seroconversion and Skin Mucosal Parameters during Koi Herpesvirus Shedding in Common Carp, Cyprinus carpio

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