Identification and functional characterization of uric acid transporter Urat1 (Slc22a12) in rats

Sato, Masanobu; Wakayama, Tomohiko; Mamada, Hideaki; Shirasaka, Yoshiyuki; Nakanishi, Takeo; Tamai, Ikumi

doi:10.1016/j.bbamem.2010.11.002

Cited by 30 publications

(34 citation statements)

References 31 publications

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“…Disruption of renal regulation of serum urate content is one of the pathological factors of hyperuricemia and gout. The analogous transport system in rats reveals 74% homology in amino acid sequence and has the same features and location, but a lower affinity for urate [45].…”

Section: Kidney Injury Markers In Obesitymentioning

confidence: 98%

“…About 90% of filtered urate is reabsorbed in the kidney; transport systems responsible for the reabsorption of urate in the nephron cells are found in both human and rats [36,[45][46][47]. The most important of them are URAT1 and GLUT9 [45][46][47].…”

Section: Kidney Injury Markers In Obesitymentioning

confidence: 99%

“…The most important of them are URAT1 and GLUT9 [45][46][47]. Disruption of renal regulation of serum urate content is one of the pathological factors of hyperuricemia and gout.…”

Section: Kidney Injury Markers In Obesitymentioning

confidence: 99%

“…Gout is a common metabolic disorder, involving the liver, kidney, and joints; it is met more often in men, and is characterized by the deposition of reversible monosodium urate (MSU) crystals and occurs as a consequence of hyperuricemia which induces inflammatory arthritis and nephropathy. Serum uric acid levels are elevated in secondary insulin resistance syndromes (e.g., gout, transplantation, pre-eclampsia, and diuretic use [45][46][47]65].…”

Section: Mets Hyperuricemia and Nephropathymentioning

confidence: 99%

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Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

et al. 2017

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Background Worldwide obesity spread is a global health problem and needs to be further studied. Co-morbidities of obesity include insulin resistance, diabetes mellitus type 2, and dyslipidemia, which are the most frequent contributing factors for metabolic syndrome (MetS), as well as nonalcoholic fatty liver disease and chronic kidney disease. The aim was to study renal function and endogenous intoxication panel on high-calorie diet-induced obesity rat model and perform comparative study of the treatment efficacy of Fenugreek-based bionanocomposite vs antiobesogenic drugs (Orlistat). Materials We included 60 male rats and equally divided them to 6 groups of 10 animals in each group: the experimental groups were firstly assigned as controls and high caloric diet (HCD)-fed groups, and each group further was subdivided to remain untreated, Fenugreek bionanocomposite (BNC)-treated, and Orlistat-treated. Normal control rats (groups 1, 2, 3) were fed by a standard chow, while the others (groups 4, 5, 6) were fed with HCD ad libitum during 98 days. From days 77 to 98, groups 2 and 5 were treated with BNC based on Fenugreek (150 mg/kg body weight, orally) and groups 3 and 6 were treated with antiobesogenic drug Orlistat (10 mg/kg body weight, orally). Food and water consumptions were measured daily and body weights were measured once a week. On day 99, blood was collected; the creatinine, urea, and uric acid were estimated in serum according to the standard protocols. Levels of low and middle molecules (MMs) were measured; the quantity of oligopeptides was estimated by Bradford method. We performed the liver and kidney ultrasonography in rats.Results We revealed an increase in the levels of endogenous intoxication syndrome markers (MM and oligopeptides) in all animals with experimental obesity. Ultrasound data showed injury of the liver and kidneys in obese rats. We observed significant decreasing of MM levels after Orlistat treatment vs controls (p < 0.05). However, this effect was more pronounced in Fenugreek BNC-treated group vs both Orlistattreated and controls (p < 0.05). Orlistat treatment evoked rising of serum creatinine and oligopeptides in control animals

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Section: Kidney Injury Markers In Obesitymentioning

confidence: 98%

Section: Kidney Injury Markers In Obesitymentioning

confidence: 99%

“…The most important of them are URAT1 and GLUT9 [45][46][47]. Disruption of renal regulation of serum urate content is one of the pathological factors of hyperuricemia and gout.…”

Section: Kidney Injury Markers In Obesitymentioning

confidence: 99%

Section: Mets Hyperuricemia and Nephropathymentioning

confidence: 99%

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Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

et al. 2017

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“…To validate the assay system, promoter region DNAs corresponding to unproximal tubules of the kidney, where it is responsible for the tubular reabsorption of urate (6,20,18). Kikuchi et al showed that the proximal promoter region of Slc22a12 was hypermethylated in the liver and kidney medulla, whereas this region was relatively hypomethylated in the kidney cortex (14).…”

Section: Development Of a Real-time Quantitative Pcr Assaymentioning

confidence: 99%

A novel biomarker for acute kidney injury using TaqMan-based unmethylated DNA-specific polymerase chain reaction

et al. 2014

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There has been increasing interest in the use of circulating DNA as biomarkers for various tissue injuries, cancers, and fetal conditions. DNA methylation is a well-characterized mechanism underlying the epigenetic regulation of gene expression, and many diagnostic tests based on DNA methylation patterns have been developed. We developed a novel TaqMan-based assay for the detection of acute kidney injury using a hypomethylated promoter region of Slc22a12, a urate transporter specifically expressed in proximal tubular cells. Bisulfite sequencing analysis confirmed that the CpG islands in the promoter region of mouse Slc22a12 were preferentially hypomethylated in the kidney cortex. TaqMan minor groove binder (MGB) probes reliably discriminated the DNA fragments corresponding to the unmethylated and methylated promoter regions of Slc22a12. Plasma levels of unmethylated DNA corresponding to the Slc22a12 promoter region were undetectable at baseline and were significantly elevated after acute kidney cortex necrosis. This study showed the usefulness of the TaqMan system in discriminating methylated and unmethylated DNA fragments, and the similar strategy can be applied for establishing biomarkers for various cellular injuries or pathological conditions.

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Osmoregulation and Excretion

Larsen

Deaton

Onken

et al. 2014

Comprehensive Physiology

181

141

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The article discusses advances in osmoregulation and excretion with emphasis on how multicellular animals in different osmotic environments regulate their milieu intérieur. Mechanisms of energy transformations in animal osmoregulation are dealt with in biophysical terms with respect to water and ion exchange across biological membranes and coupling of ion and water fluxes across epithelia. The discussion of functions is based on a comparative approach analyzing mechanisms that have evolved in different taxonomic groups at biochemical, cellular and tissue levels and their integration in maintaining whole body water and ion homeostasis. The focus is on recent studies of adaptations and newly discovered mechanisms of acclimatization during transitions of animals between different osmotic environments. Special attention is paid to hypotheses about the diversity of cellular organization of osmoregulatory and excretory organs such as glomerular kidneys, antennal glands, Malpighian tubules and insect gut, gills, integument and intestine, with accounts on experimental approaches and methods applied in the studies. It is demonstrated how knowledge in these areas of comparative physiology has expanded considerably during the last two decades, bridging seminal classical works with studies based on new approaches at all levels of anatomical and functional organization. A number of as yet partially unanswered questions are emphasized, some of which are about how water and solute exchange mechanisms at lower levels are integrated for regulating whole body extracellular water volume and ion homeostasis of animals in their natural habitats. © 2014 American Physiological Society.

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Identification and functional characterization of uric acid transporter Urat1 (Slc22a12) in rats

Cited by 30 publications

References 31 publications

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

Efficacy of Fenugreek-based bionanocomposite on renal dysfunction and endogenous intoxication in high-calorie diet-induced obesity rat model—comparative study

A novel biomarker for acute kidney injury using TaqMan-based unmethylated DNA-specific polymerase chain reaction

Osmoregulation and Excretion

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