Identification and functional characterization of two alternatively spliced growth hormone secretagogue receptor transcripts from the pituitary of black seabream Acanthopagrus schlegeli

Sequence, genomic organization and expression of ghrelin receptor in grass carp, Ctenopharyngodon idellus

“…This gene organization and the insert position of the intron are identical to those of the mammalian GHS-R gene (McKee et al, 1997;Petersenn, 2002), indicating that the GHSR gene has been evolutionary conserved from fish to mammals. Seven TMD were predicted in grass carp GHS-R1a and this organization was identical to that reported for other GHS-R1 genes including those of human (Petersenn et al, 2001), chicken (Tanaka et al, 2003) and other fishes (Palyha et al, 2000;Chan and Cheng, 2004;Olsson et al, 2008;Kaiya et al, 2009a,b).…”

“…Amino acid sequences of GHS-R1a of seabream, pufferfish and chicken GHS-R1a have 56, 55 and 72% identity to human GHS-R1a, respectively (Palyha et al, 2000;Kaiya et al, 2008;Zhang et al, 2008). It has been reported that the GHS-R gene has two exons and one intron in chicken (Tanaka et al, 2003), seabream (Chan and Cheng, 2004) and pufferfish (Palyha et al, 2000). This gene organization and the insert position of the intron are identical to those of the mammalian GHS-R gene (McKee et al, 1997;Petersenn, 2002), indicating that the GHSR gene has been evolutionary conserved from fish to mammals.…”

“…Mammalian GHS-R gene is composed of two exons, and two types of GHS-R mRNAs, GHS-R1a and 1b, are generated by alternative transcription process of the gene (Howard et al, 1996). So far, GHS-Rs have been identified in mammals (Howard et al, 1996;Katayama et al, 2000;Kitazawa et al, 2011;Suzuki et al, 2012), avians (Geelissen et al, 2003;Tanaka et al, 2003) and several fish species (Palyha et al, 2000;Chan and Cheng, 2004;Olsson et al, 2008;Kaiya et al, 2009a,b;Small et al, 2009;Eom et al, 2014). In fish, GHS-R1a was first identified by Palyha et al (2000) in pufferfish Takifugu rubripes.…”

“…In fish, GHS-R1a was first identified by Palyha et al (2000) in pufferfish Takifugu rubripes. After that, Chan and Cheng (2004) identified two isoforms of GHS-R in black seabream Acanthopagrus schlegeli, sbGHSR-1a and sbGHSR-1b. It was the first report on the identification of a GHSR-1b transcript from species other than mammals and the demonstration that receptor interaction might provide a possible explanation for the existence and biological significance of the sbGHSR-1b transcript.…”

Ghrelin modulates hypothalamic fatty acid-sensing and control of food intake in rainbow trout

“…It is highly expressed in the gastrointestinal tract, moderately in the brain and to a lower extent in other tissues (Unniappan et al 2002, Volkoff et al 2005. Once octanoylated, GHRL binds to GHS-R1a, which is also expressed in a panel of fish tissues such as the brain (particularly hypothalamus), gastrointestinal tissue, pancreatic cells and liver (Chang & Cheng 2004, Kaiya et al 2009, Cruz et al 2010. The role of GHRL in fish is far from elucidated though it has been involved in the control of food intake (Unniappan et al 2002, Kang et al 2011, Jönsson 2013), glucose and lipid metabolism (Kaiya et al 2009, Salmeró n et al 2015 and the modulation of growth hormone and prolactin release (Kaiya et al 2008).…”