Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci

Lueke, Bettina; Douris, Vassilis; Hopkinson, Jamie; Maiwald, Frank; Hertlein, Gillian; Papapostolou, Kyriaki Maria; Bielza, Pablo; Tsagkarakou, Anastasia; Leeuwen, Thomas Van; Bass, Chris; Vontas, John; Nauen, Ralf

doi:10.1016/j.pestbp.2020.104583

Cited by 31 publications

(61 citation statements)

References 48 publications

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“…This mutation is located outside the CT domain and genome editing in the model organism Drosophila melanogaster did not support a role in resistance to keto‐enols 78 . Recently, a novel point mutation (A2083V) was identified in the CT domain of ACCase in Bemisia tabaci and genome editing in D. melanogaster confirmed high resistance levels to keto‐enols 78 . Here, we did not identify any non‐synonymous mutations in the CT domain, whereas two mutations, T1158N and T2315L, with the latter not being fixed, were identified in less‐conserved regions.…”

Section: Discussionmentioning

confidence: 87%

“…Spirodiclofen targets ACCase and at present, only one mutation (A1079T) has been identified in the ACCase gene of a spirodiclofen‐resistant strain of T. urticae 77 . This mutation is located outside the CT domain and genome editing in the model organism Drosophila melanogaster did not support a role in resistance to keto‐enols 78 . Recently, a novel point mutation (A2083V) was identified in the CT domain of ACCase in Bemisia tabaci and genome editing in D. melanogaster confirmed high resistance levels to keto‐enols 78 .…”

Section: Discussionmentioning

confidence: 94%

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Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

Papapostolou

Riga

Charamis

et al. 2020

Pest Management Science

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BACKGROUND Tetranychus urticae is a notorious crop pest with a worldwide distribution that has developed resistance to a wide range of acaricides. Here, we investigated the resistance levels of a T. urticae population collected from an ornamental greenhouse in Peloponnese, Greece, and analyzed its resistance mechanisms at the molecular level. RESULTS Toxicological assays showed resistance against compounds with different modes of action, with resistance ratios of: 89‐fold for abamectin; > 1000‐fold for clofentezine; > 5000‐fold for etoxazole; 27‐fold for fenpyroximate and pyridaben; 20‐ and 36‐fold for spirodiclofen and spirotetramat, respectively; and 116‐ and > 500‐fold for cyenopyrafen and cyflumetofen, respectively. Bioassays with synergists indicated the involvement of detoxification enzymes in resistance to abamectin, but not to cyflumetofen and spirodiclofen. RNA sequencing (RNA‐seq) analysis showed significant over‐expression of several genes encoding detoxification enzymes such as cytochrome P450 monooxygenases and UDP‐glycosyltransferases, which have been previously associated with acaricide resistance. Known target‐site resistance mutations were identified in acetyl‐choline esterase, chitin synthase 1 and NDUFS7/psst, but putative novel resistance mutations were also discovered in targets such as glutamate‐gated chloride channel subunit 3. Interestingly, target‐site resistance mutations against pyrethroids or bifenazate were not identified, possibly indicating a recent reduced selection pressure in Greece, as well as a possible opportunity to rotate these chemistries. CONCLUSION We identified and characterized a striking case of multiple acaricide resistance in a field population of T. urticae. Exceptionally strong resistance phenotypes, with accumulation of multiple resistance mutations and over‐expression of P450s and other detoxification genes in the same field population are reported.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 94%

Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

Papapostolou

Riga

Charamis

et al. 2020

Pest Management Science

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“…Such cases of robust molecular markers do not exist only in T.urticae but are also in place for other major agricultural pests. Examples include the A2083V mutation in acetyl‐CoA carboxylase conferring ketoenol resistance in Bemisia tabaci , 49 and the G4946V mutation in T. absoluta providing high levels of resistance to diamides as shown by in vivo functional assays 50 . Thus, similar ddPCR panels can be developed for those pests.…”

Section: Discussionmentioning

confidence: 99%

Multiple TaqMan qPCR and droplet digital PCR (ddPCR) diagnostics for pesticide resistance monitoring and management, in the major agricultural pest Tetranychus urticae

Mavridis

Papapostolou

Riga

et al. 2021

Pest Management Science

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BACKGROUND Decisions on which pesticide to use in agriculture are expected to become more difficult, as the number of available chemicals is decreasing. For Tetranychus urticae (T. urticae), a major pest for which a number of candidate markers for pesticide resistance are in place, molecular diagnostics could support decision‐making for the rational use of acaricides. RESULTS A suite of 12 TaqMan qPCR assays [G314D (GluCl1), G326E, I321T (GluCl3), G119S, F331W (Ace‐1), H92R (PSST), L1024V, F1538I (VGSC), I1017F (CHS1), G126S, S141F, P262T (cytb)], were validated against Sanger‐sequencing, and subsequently adapted for use with the ddPCR technology. The concordance correlation coefficient between the actual and ddPCR measured mutant allelic frequencies was 0.995 (95% CI = 0.991–0.998), and no systematic, proportional, or random differences were detected. The achieved Limit of Detection (LoD) was 0.1% (detection of one mutant in a background of 999 wild type mites). The ddPCR assay panel was then assessed in terms of agreement with phenotypic resistance, through a pilot application in field populations from Crete, with strong correlation and thus predictive and diagnostic value of the molecular assays in some cases (e.g., etoxazole and abamectin resistance). Molecular diagnostics were able to capture incipient resistance that was otherwise missed by phenotypic bioassays. The molecular and phenotypic resistance screening of T. urticae field populations from Crete, revealed both multi‐resistant and susceptible populations. CONCLUSION The highly sensitive T. urticae molecular diagnostic platforms developed in this study could prove a valuable tool for pesticide resistance management. © 2021 Society of Chemical Industry.

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“…Similarly, recent evolution resistance in Bemisia tabaci to derivatives of tetramic and tetronic acid (Lueke et al . 2020), and pyrethroid resistance in H. destructor (Edwards et al . 2018; Yang et al .…”

Section: Discussionmentioning

confidence: 99%

“…For example, research towards characterising H. armigera's genetic mechanisms for oxadiazine resistance has progressed at a fast pace since it was first discovered (Bird et al 2017), narrowing the list of candidates to a cluster of P450 genes using genome-wide association analyses (Chen et al 2020). Similarly, recent evolution resistance in Bemisia tabaci to derivatives of tetramic and tetronic acid (Lueke et al 2020), and pyrethroid resistance in H. destructor (Edwards et al 2018;Yang et al 2020), has been rapidly characterised at the genetic level, largely due to the use of modern genomic approaches.…”

Section: Discussionmentioning

confidence: 99%

Empowering Australian insecticide resistance research with genetic information: the road ahead

2020

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Insecticides are important for chemical control of arthropod pests in agricultural systems but select for resistance as an adaptive trait. Identifying the genetic mechanism(s) underpinning resistance can facilitate development of genetic markers, which can be used in monitoring programs. Moreover, understanding of genetic mechanisms in a broader population genetic context can be used to infer the origins of resistance, predict the dynamics of resistance evolution and evaluate the efficacy of different management strategies. Transitioning genetic information successfully into practical solutions requires overcoming two major hurdles. Firstly, genetic mechanisms must be identified to develop genetic markers. Secondly, routine use of genetic markers is required to build substantial spatio‐temporal data on the distribution and frequency of resistance alleles. In this study, we demonstrate large knowledge gaps on the genetic mechanisms of insecticide resistances in Australia using eight established arthropod pests important to the grains industry: Bemisia tabaci (silverleaf whitefly), Frankliniella occidentalis (western flower thrips), Halotydeus destructor (redlegged earth mite), Helicoverpa armigera (cotton bollworm), Myzus persicae (green peach aphid), Plutella xylostella (diamondback moth), Tetranychus urticae (two‐spotted spider mite) and Thrips tabaci (onion thrips). Many resistances have not been characterised at the genetic level in most pests, even for chemical MoA groups with a long history of use in Australia. Moreover, monitoring of resistance is spatio‐temporally patchy, which precludes examination of long‐term trends or predictive modelling. We suggest that leveraging cumulative global knowledge of resistances to develop a priori candidate genes, and incorporation of genomic approaches, can help overcome the hurdles of embracing genetic information in resistance management. We highlight the recently invasive Spodoptera frugiperda (fall armyworm) as a case study where genetic markers and genomic approaches should prove useful in rapidly assessing the risk of this species to the Australian grains industry and other agricultural commodities. The uptake of genetic information into management can only occur once its benefit to empower insecticide resistance research is fully realised. Ultimately, the road ahead requires amalgamation of multifaceted data (genes, environment and spatio‐temporal replication) to better understand and predict the dynamics of resistance evolution.

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Identification and functional characterization of a novel acetyl-CoA carboxylase mutation associated with ketoenol resistance in Bemisia tabaci

Cited by 31 publications

References 48 publications

Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

Identification and characterization of striking multiple‐insecticide resistance in a Tetranychus urticae field population from Greece

Multiple TaqMan qPCR and droplet digital PCR (ddPCR) diagnostics for pesticide resistance monitoring and management, in the major agricultural pest Tetranychus urticae

Empowering Australian insecticide resistance research with genetic information: the road ahead

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