Identification and functional annotation of m6A methylation modification in granulosa cells during antral follicle development in pigs

Cao, Zubing; Zhang, Dandan; Wang, Yiqing; Xu, Tong; Avalos, Lourdes Felicidad Córdova; Khan, Ibrar Muhammad; Gao, Di; Xu, Tengteng; Zhang, Ling; Knott, Jack H.

doi:10.1016/j.anireprosci.2020.106510

Cited by 19 publications

(15 citation statements)

References 38 publications

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“…m 6 A modification plays an important role in the development of oocytes and follicles ( Figure 6 ). There are dynamic changes in m 6 A methylomes during the developmental transition from small to large sized follicles ( Cao et al, 2020 ). The loss of m 6 A compromises gamete maturation and gametogenesis processes ( Lasman et al, 2020 ; Fang et al, 2021 ).…”

Section: A Rnas In Oogenesis and Folliculogenesismentioning

confidence: 99%

“…Finally, a large number of m 6 A modifications have been found in porcine granulosa cells, which may be related to steroids synthesis and follicle development ( Cao et al, 2020 ). In the process of follicle selection that precedes ovulation, it was found that m 6 A is widely distributed in the follicular transcriptome, and methylation enrichment is negatively correlated with gene expression ( Fan et al, 2019 ).…”

Section: A Rnas In Oogenesis and Folliculogenesismentioning

confidence: 99%

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Genetic Regulation of N6-Methyladenosine-RNA in Mammalian Gametogenesis and Embryonic Development

Chang

Man

Wang

et al. 2022

Front. Cell Dev. Biol.

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Emerging evidence shows that m6A is the most abundant modification in eukaryotic RNA molecules. It has only recently been found that this epigenetic modification plays an important role in many physiological and pathological processes, such as cell fate commitment, immune response, obesity, tumorigenesis, and relevant for the present review, gametogenesis. Notably the RNA metabolism process mediated by m6A is controlled and regulated by a series of proteins termed writers, readers and erasers that are highly expressed in germ cells and somatic cells of gonads. Here, we review and discuss the expression and the functional emerging roles of m6A in gametogenesis and early embryogenesis of mammals. Besides updated references about such new topics, readers might find in the present work inspiration and clues to elucidate epigenetic molecular mechanisms of reproductive dysfunction and perspectives for future research.

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Section: A Rnas In Oogenesis and Folliculogenesismentioning

confidence: 99%

Section: A Rnas In Oogenesis and Folliculogenesismentioning

confidence: 99%

Genetic Regulation of N6-Methyladenosine-RNA in Mammalian Gametogenesis and Embryonic Development

Chang

Man

Wang

et al. 2022

Front. Cell Dev. Biol.

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“…A growing number of studies indicated that m 6 A play crucial roles in the development of reproductive organ [32], gametogenesis [33], fertilization [34] and early embryonic development [7,35]. Cao et al found that there exist abundant m 6 A modi cation sites in genes for porcine granulosa cells [36]. The differentially expressed m 6 A methylated genes were signi cantly enriched in several signaling pathways related with steroidogenesis, granulosa cell proliferation and follicular development [36].…”

Section: Discussionmentioning

confidence: 99%

“…Cao et al found that there exist abundant m 6 A modi cation sites in genes for porcine granulosa cells [36]. The differentially expressed m 6 A methylated genes were signi cantly enriched in several signaling pathways related with steroidogenesis, granulosa cell proliferation and follicular development [36]. Wang et al, reported that CL treatment impaired the maturation of porcine oocytes and reducing the RNA m 6 A level [37].…”

Section: Discussionmentioning

confidence: 99%

m6a Methylation Inhibition by Cycloleucine Impaired Porcine Oocyte Meiosis and Early Embryonic Development via Remodeling Histone Modifications and Altering Metabolism Related Gene Expression in Blastocysts

Zhang¹,

Zhang²,

Zhai³

et al. 2020

Preprint

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BackgroundOocytes maturation and early embryo development were regulated precisely by a series of factors at transcriptional and posttranslational levels. N6-methyladenosine (m6A) is the most prevalent modification in mRNA as a crucial regulator in RNA metabolism and gene regulation. However, the role of m6A on porcine oocyte maturation and early embryogenesis is largely unknown. ResultsHere, we found that oocytes treated with cycloleucine (CL), an inhibitor of m6A, could impair cumulus expansion, elevate mitochondrial reactive oxygen species (ROS) concentration and decreased oocytes maturation which partially caused by disturbed spindle organization and chromosomes alignment. Moreover, our results indicated that the CL treated parthenogenetic embryos arrested at 4-cell stage and showed worse blastocyst quality. CL treatment not only decreased the methylation levels of nucleic acid, H3K4me3 and H3K9me3, while increased the acetylation level of H4K16 during parthenogenetic embryos development in pigs. Furthermore, single cell RNA-seq (scRNA-seq) analysis indicated that CL treatment dramatically elevated the expression of metabolism-related (SLC16A1 and MAIG3 etc.) and maternal related (BTG4, WEE2 and BMP15 etc.) genes at blastocyst stage. ConclusionsTaken together, we found that m6A methylation inhibition by CL impaired porcine oocyte meiosis and early embryonic development via remodeling histone modifications and altering metabolism related gene expression in blastocysts.

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“…Follicles provide a developmental microenvironment for oocyte growth and help sustain meiotic arrest [89,90]. MeRIP-seq identified changes in the abundance and distribution of m 6 A modifications in granulosa cells during follicular development [91].…”

Section: Follicle Developmentmentioning

confidence: 99%

N6-Methyladenosine Modifications in the Female Reproductive System: Roles in Gonad Development and Diseases

Mu¹,

Li²,

Liu³

et al. 2022

Int. J. Biol. Sci.

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N6-methyladenosine (m 6 A) is the most prevalent chemical modification in eukaryotic messenger RNAs. By participating in various RNA-related bioprocesses including RNA decay, splicing, transport and translation, m 6 A serves as a pivotal regulator of RNA fate and plays an irreplaceable role in cellular activities. The m 6 A modifications of transcripts are coordinately regulated by methyltransferase "writers" and demethylase "erasers", and produce variable effects via different m 6 A reading protein "readers". There is emerging evidence that m 6 A modifications play a critical role in a variety of physiological and pathological processes in the female reproductive system, subsequently affecting female fertility. Here, we introduce recent advances in research on m 6 A regulators and their functions, then highlight the role of m 6 A in gonad development and female reproductive diseases, as well as the underlying mechanisms driving these processes.

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Identification and functional annotation of m6A methylation modification in granulosa cells during antral follicle development in pigs

Cited by 19 publications

References 38 publications

Genetic Regulation of N6-Methyladenosine-RNA in Mammalian Gametogenesis and Embryonic Development

Genetic Regulation of N6-Methyladenosine-RNA in Mammalian Gametogenesis and Embryonic Development

m6a Methylation Inhibition by Cycloleucine Impaired Porcine Oocyte Meiosis and Early Embryonic Development via Remodeling Histone Modifications and Altering Metabolism Related Gene Expression in Blastocysts

N6-Methyladenosine Modifications in the Female Reproductive System: Roles in Gonad Development and Diseases

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