Identification and expression profiling of toxic boron-responsive microRNAs and their targets in sensitive and tolerant wheat cultivars

Abstract

Post transcriptional gene regulation in wheat cultivars caused by boron (B) toxicity has not been reported to date. In this study, two wheat cultivars Bolal-2973 (B-tolerant) and Atay-85 (Bsensitive) were compared with regard to the transcriptional regulation of miR319, miR172 and miR398 and their plausible target genes in order to address differences in their performances under high B levels. The expression levels of Cu/ZnSOD gene were found to be greater in Atay exposed to toxic B compared to Bolal, as verified by stable expression level of miR398. In wheat cultivars, both toxic B concentrations might cause an induction of leaf senescence mechanism due to stable level of JA and ethylene-related miRNAs, miR172 and miR319. miR172 targeting transcription factor TOE1 was only induced under B toxicity in sensitive cultivar Atay. However, MYB3 as target of miR319 were significantly upregulated under toxic B in both cultivars. Also, computational prediction of these miRNA targets in wheat was performed and their orthologs in Arabidopsis thaliana were determined. Functional protein association networks of proteins encoded by miRNA targets and gene ontology enrichment analyses of these genes were identified. We identified new sets of genes that are targets of miR172, miR319 and miR398 in T. aestivum. In addition, miR172, miR319 and miR398 are responsive to different nutrient deficiencies or toxicities such as Fe, P, B, S and Cu, suggesting crosstalk between the post-transcriptional regulatory mechanisms involving miRNAs in plants.

MicroRNA Mediated Plant Responses to Nutrient Stress

“…According to new research, Triticum aestivum is home to several genes whose targets are miR172, miR319, and miR398. Furthermore, due to interaction amongst miRNA-mediated post transcriptional pathways, miR172, miR319, and miR398 are extremely susceptible to nutritional shortages or toxicities, such as those of Fe, P, B, S, and Cu [ 107 ].…”

Physiology and transcriptome analysis of the response mechanism of Solidago canadensis to the nitrogen addition environment

“…With the development of biology, next-generation RNA sequencing technologies (RNA-seq) have supplied an approach to describe the whole genome or transcriptomes and are being used to explore the regulatory mechanisms of plant responses to many conditions in which they grow ( Chu et al., 2019 ; Khan et al., 2022 ). Therefore, transcriptomic profiling can be used as an emerging method to reveal the molecular mechanism of plants in response to different environments ( Asim et al., 2021 ; Kayihan et al., 2021 ; Qu et al., 2021 ; Li et al., 2022 ).…”

The Penicillium brasilianum Histone Deacetylase Clr3 Regulates Secondary Metabolite Production and Tolerance to Oxidative Stress

“…Multiple factors regulate gene expression in a given condition, including chromatin packing. Histone modifications play an essential role in altering chromatin structure and, therefore, regulating transcription [ 15 , 16 ]. Histone acetylation is the most studied histone modification and depends on the concerted action of histone acetyltransferases (HATs) and histone deacetylases (HDACs) [ 17 ].…”