Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori

Gong, Daping; Zhang, Huijie; Zhao, Ping; Lin, Ying; Xia, Qingyou; Zhang, Xiang

doi:10.1016/j.ibmb.2006.11.012

Cited by 156 publications

(183 citation statements)

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“…Earlier studies have shown that many insect CSPs are ancient and shared by different insect orders Gong et al, 2007;Xu et al, 2009). However, including data from several arthropod genomes led Vieira and Rozas (2011) to conclude that the CSP family is more dynamic in terms of gene loss and gain than previously found.…”

Section: Discussionmentioning

confidence: 99%

“…The abbreviations used here are, respectively, Cflo, Hsal, Sinv, Lhum, Pbar, Aech and Acep. We first searched the predicted protein models of each ant species using the protein sequences of insect CSPs Gong et al, 2007;González et al, 2009;Xu et al, 2009;Vieira and Rozas, 2011) and e-value of 1.0 e-3. Adding the hits of the first search to queries, we searched the ant genome assemblies using tblastn.…”

Section: Csp Gene Identificationmentioning

confidence: 99%

“…The odorant receptor family varies in size from 54 genes in Drosophila virilis to 299 in the flour beetle Tribolium castaneum (Nozawa and Nei, 2007;Engsontia et al, 2008). The OBP and CSP gene families are smaller, OBP family containing 4-81 genes and CSP 3-22 genes in the insect species studied this far (Forêt and Maleszka, 2006;Forêt et al, 2007;Gong et al, 2007;Vieira and Rozas, 2011). OBPs and CSPs belong to different gene families, but possibly have common evolutionary origin of 380-450 million years ago (Mya) .…”

Section: Introductionmentioning

confidence: 99%

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Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

2013

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Gene duplications can have a major role in adaptation, and gene families underlying chemosensation are particularly interesting due to their essential role in chemical recognition of mates, predators and food resources. Social insects add yet another dimension to the study of chemosensory genomics, as the key components of their social life rely on chemical communication. Still, chemosensory gene families are little studied in social insects. Here we annotated chemosensory protein (CSP) genes from seven ant genomes and studied their evolution. The number of functional CSP genes ranges from 11 to 21 depending on species, and the estimated rates of gene birth and death indicate high turnover of genes. Ant CSP genes include seven conservative orthologous groups present in all the ants, and a group of genes that has expanded independently in different ant lineages. Interestingly, the expanded group of genes has a differing mode of evolution from the orthologous groups. The expanded group shows rapid evolution as indicated by a high dN/dS (nonsynonymous to synonymous changes) ratio, several sites under positive selection and many pseudogenes, whereas the genes in the seven orthologous groups evolve slowly under purifying selection and include only one pseudogene. These results show that adaptive changes have played a role in ant CSP evolution. The expanded group of ant-specific genes is phylogenetically close to a conservative orthologous group CSP7, which includes genes known to be involved in ant nestmate recognition, raising an interesting possibility that the expanded CSPs function in ant chemical communication.

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Section: Discussionmentioning

confidence: 99%

Section: Csp Gene Identificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

2013

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“…In fact, CSPs have been described that are upregulated during molting of the Eastern spruce budworm (Wanner et al, 2005) and that are integral to synthesis of the honeybee embryonic integument , supporting a role for these genes in development. A diverse functional role for this family of proteins is also proposed due to its expression in various tissues, such as pheromone glands (Gong et al, 2007) and the Drosophila ejaculatory duct (PebIII), and makes them attractive as targets for control.…”

Section: Discussionmentioning

confidence: 99%

Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

Olafson

Lohmeyer

Dowd

2010

Arch Insect Biochem Physiol

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The stable fly, Stomoxys calcitrans L. (Diptera: Muscidae), is one of the most significant pests of livestock in the United States. The identification of targets for the development of novel control for this pest species, focusing on those molecules that play a role in successful feeding and reproduction, is critical to mitigating its impact on confined and rangeland livestock. A database was developed representing genes expressed at the immature and adult life stages of the stable fly, comprising data obtained from pyrosequencing both immature and adult stages and from small-scale sequencing of an antennal/maxillary palp-expressed sequence tag library. The full-length sequence and expression of 21 transcripts that may have a role in chemosensation is presented, including 13 odorant-binding proteins, 6 chemosensory proteins, and 2 odorant receptors. Transcripts with potential roles in sex determination and reproductive behaviors are identified, including evidence for the sex-specific expression of stable fly doublesex- and transformer-like transcripts. The current database will be a valuable tool for target identification and for comparative studies with other Diptera.

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“…In addition to receptor genes, other multigene families encode proteins such as odorant binding proteins (OBPs) and chemosensory proteins (CSPs), which are gene families with critical roles in taste and olfaction (Andersson et al, 2013). The small, water soluble OBPs within the lymphatic cavity of olfactory sensilla that facilitate ligand binding to ORs (Gong et al, 2009) but CSPs may help mediate binding between ligands and receptors (Gong et al, 2007).…”

Section: Rt-pcr For Expression Analysismentioning

confidence: 99%

Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae

Ahmed¹,

Zhang²,

Wang³

et al. 2016

Eur. J. Entomol.

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Abstract.Macrocentrus cingulum is an important polyembryonic endoparasitic wasp that attacks larvae of the Asian corn borer, Ostrinia furnacalis (Guenée) and the European corn borer, O. nubilalis (Hübner). Parasitoids use antennae as the main sensory organ to recognize herbivore-induced plant volatiles as host searching cues. The antennal olfaction proteins, odorant receptors (ORs) and ionotropic receptors (IRs) are involved in olfactory signal transduction pathway as a sensory neuron response. In the present study, we constructed a cDNA library from the male and female antennae for identifying the olfaction-related genes in M. cingulum. For that, we sequenced 3160 unique gene sequences and annotated them with gene ontology (GO), cluster of orthologous groups of proteins (COG), and KEGG ontology (KO). Through the homology search, we identifi ed 9 odorant receptors (ORs), 3 ionotropic receptors (IRs) and 1 odorant binding protein (OBP) genes from the cDNA library sequences. Additionally, the expression patterns of these ORs and IRs in different tissues (antennae, heads, thoraxes, abdomens, and legs) were demonstrated by RT-PCR. The qualitative gene expression analyses showed that most of the OR genes were more highly expressed in female than male antennae; whereas IRs, unlike ORs, were more expressed in various male than females tissues. We are the fi rst to report ORs and IRs in M. cingulum, which should help in deciphering the molecular basis of olfaction system in this wasp.

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Identification and expression pattern of the chemosensory protein gene family in the silkworm, Bombyx mori

Cited by 156 publications

References 53 publications

Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

Comparative genomics of chemosensory protein genes reveals rapid evolution and positive selection in ant-specific duplicates

Analysis of expressed sequence tags from a significant livestock pest, the stable fly (Stomoxys calcitrans), identifies transcripts with a putative role in chemosensation and sex determination

Identification and expression pattern analysis of chemosensory receptor genes in the Macrocentrus cingulum (Hymenoptera: Braconidae) antennae

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