Identification and expression pattern analysis of BoMYB51 involved in indolic glucosinolate biosynthesis from broccoli (Brassica oleracea var. italica)

Yu, Qingyue; Hao, Guodong; Zhou, Jianxin; Wang, Jingying; Evivie, Ejiroghene Ruona; Li, Jing

doi:10.1016/j.bbrc.2018.05.058

Cited by 14 publications

(6 citation statements)

References 32 publications

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“…The core structure biosynthesis genes for Phe-derived GSLs, Trp-derived indolic GSLs, Met-derived aliphatic GSLs, and the side-chain elongation genes for phenethylGSL and Met-derived GSLs are shared among different GSLs, the transcription factors regulating different GSLs biosynthesis were also shared [6]. It was verified that MYB34, MYB51 and MYB122 regulate indolic GSLs biosynthesis in A. thaliana and B. oleracea [17,40,41]. MYB28 was verified controlling aliphatic GSLs biosynthesis in B. juncea and B. napus [14,23], which also potentially regulated benzylGSL biosynthesis in leaf and stem of Sinapis alba [25].…”

Section: Discussionmentioning

confidence: 83%

Genome-Wide Association Study of Phenylalanine Derived Glucosinolates in Brassica rapa

2022

Plants

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Glucosinolates (GSLs) are sulfur-containing bioactive compounds usually present in Brassicaceae plants and are usually responsible for a pungent flavor and reduction of the nutritional values of seeds. Therefore, breeding rapeseed varieties with low GSL levels is an important breeding objective. Most GSLs in Brassica rapa are derived from methionine or tryptophan, but two are derived from phenylalanine, one directly (benzylGSL) and one after a round of chain elongation (phenethylGSL). In the present study, two phenylalanine (Phe)-derived GSLs (benzylGSL and phenethylGSL) were identified and quantified in seeds by liquid chromatography and mass spectrometry (LC-MS) analysis. Levels of benzylGSL were low but differed among investigated low and high GSL genotypes. Levels of phenethylGSL (also known as 2-phenylethylGSL) were high but did not differ among GSL genotypes. Subsequently, a genome-wide association study (GWAS) was conducted using 159 B. rapa accessions to demarcate candidate regions underlying 43 and 59 QTNs associated with benzylGSL and phenethylGSL that were distributed on 10 chromosomes and 9 scaffolds, explaining 0.56% to 70.86% of phenotypic variations, respectively. Furthermore, we find that 15 and 18 known or novel candidate genes were identified for the biosynthesis of benzylGSL and phenethylGSL, including known regulators of GSL biosynthesis, such as BrMYB34, BrMYB51, BrMYB28, BrMYB29 and BrMYB122, and novel regulators or structural genes, such as BrMYB44/BrMYB77 and BrMYB60 for benzylGSL and BrCYP79B2 for phenethylGSL. Finally, we investigate the expression profiles of the biosynthetic genes for two Phe-derived GSLs by transcriptomic analysis. Our findings provide new insight into the complex machinery of Phe-derived GSLs in seeds of B. rapa and help to improve the quality of Brassicaceae plant breeding.

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Section: Discussionmentioning

confidence: 83%

Genome-Wide Association Study of Phenylalanine Derived Glucosinolates in Brassica rapa

2022

Plants

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“…pekinensis (Kim et al, 2013 ), B. juncea (Augustine et al, 2013 ), and B. oleracea (Araki et al, 2013 ; Yin et al, 2017 ), none of the transcription factors regulating indole GSL in Brassica crops have been well investigated to date. Previous study only identified one BoMYB51 from broccoli (Yu et al, 2018 ). Here, we report that both two BoaMYB51 genes have been isolated with identification of their transcriptional activation domain (TAD) and novel protein interactors.…”

Section: Introductionmentioning

confidence: 98%

Functional Characterization of BoaMYB51s as Central Regulators of Indole Glucosinolate Biosynthesis in Brassica oleracea var. alboglabra Bailey

et al. 2018

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R2R3-MYB transcription factor MYB51 is known to control indole glucosinolate (indole GSL) biosynthesis in Arabidopsis. Here, two copies of BoaMYB51 have been isolated in Chinese kale (Brassica oleracea var. alboglabra Bailey), designated BoaMYB51.1 and BoaMYB51.2, which exhibit overlapping but distinct expression levels among different organs and respond to signaling molecules in a similar pattern. It has been demonstrated a structural and functional conservation between BoaMYB51s and AtMYB51 by phylogenetic analysis, complementation studies and transient expression assay. To further investigate the transcriptional mechanism, we identified the transcriptional activation domain (TAD) and putative interacting proteins of BoaMYB51s by means of yeast (Saccharomyces cerevisiae) two hybrid. Using tobacco (Nicotiana benthamiana) transient expression assay, we confirmed that the carboxy-end is required for transcriptional activation activity of BoaMYB51s. In addition, several BoaMYB51-interacting proteins have been identified by yeast two-hybrid screening. These results provide important insights into the molecular mechanisms by which MYB51 transcriptionally regulates indole GSL biosynthesis.

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“…Based on different AA sources of R groups, GSLs can be divided into three categories: aliphatic GSLs (R side chains are derived from methionine, alanine, valine, leucine, and isoleucine), aromatic GSLs (R side chains are derived from amino acids containing aromatic rings, tyrosine, and phenylalanine), and indole GSLs (R side chains are derived from tryptophan). The GSL synthetic pathway has been thoroughly studied in the model plant Arabidopsis thaliana [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. The biosynthesis of glucosinolates can be summarized into three stages: extension of the R side chain, synthesis of the core structure, and modification of the side chain [39] (Figure 1) Figure 1 is quoted from references [40,41].…”

Section: Introductionmentioning

confidence: 99%

Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis

Wang

Cao

Yang

et al. 2023

Genes

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Cabbage (Brassica oleracea var. capitata) is a vegetable rich in glucosinolates (GSLs) that have proven health benefits. To gain insights into the synthesis of GSLs in cabbage, we systematically analyzed GSLs biosynthetic genes (GBGs) in the entire cabbage genome. In total, 193 cabbage GBGs were identified, which were homologous to 106 GBGs in Arabidopsis thaliana. Most GBGs in cabbage have undergone negative selection. Many homologous GBGs in cabbage and Chinese cabbage differed in expression patterns indicating the unique functions of these homologous GBGs. Spraying five exogenous hormones significantly altered expression levels of GBGs in cabbage. For example, MeJA significantly upregulated side chain extension genes BoIPMILSU1-1 and BoBCAT-3-1, and the expression of core structure construction genes BoCYP83A1 and BoST5C-1, while ETH significantly repressed the expression of side chain extension genes such as BoIPMILSU1-1, BoCYP79B2-1, and BoMAMI-1, and some transcription factors, namely BoMYB28-1, BoMYB34-1, BoMYB76-1, BoCYP79B2-1, and BoMAMI-1. Phylogenetically, the CYP83 family and CYP79B and CYP79F subfamilies may only be involved in GSL synthesis in cruciferous plants. Our unprecedented identification and analysis of GBGs in cabbage at the genome-wide level lays a foundation for the regulation of GSLs synthesis through gene editing and overexpression.

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Identification and expression pattern analysis of BoMYB51 involved in indolic glucosinolate biosynthesis from broccoli (Brassica oleracea var. italica)

Cited by 14 publications

References 32 publications

Genome-Wide Association Study of Phenylalanine Derived Glucosinolates in Brassica rapa

Genome-Wide Association Study of Phenylalanine Derived Glucosinolates in Brassica rapa

Functional Characterization of BoaMYB51s as Central Regulators of Indole Glucosinolate Biosynthesis in Brassica oleracea var. alboglabra Bailey

Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis

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