Identification and expression of DoCCaMK during Sebacina sp. symbiosis of Dendrobium officinale

Abstract

Dendrobium officinale Kimura et Migo is a famous precious medicinal plant in China. Seed and seedling were cultivated with the mycorrhizal fungus Sebacina sp. CCaMK was initially cloned from D. officinale based on a SSH cDNA library of symbiotically germinated seeds with Sebacina sp. Phylogenetic analysis was performed among DoCCaMK and other CCaMKs. The particle bombardment technique was used to visualize DoCCaMK-GFP. qRT-PCR and western blot analysis were conducted to determine the tissue expression patterns of DoCCaMK with (SGS) and without (UGS) Sebacina sp. Furthermore, the effect of KN-93 on CCaMK expression was also examined. Using NMT the net Ca2+ fluxes and the CCaMK concentration were measured during D. officinale seed germination. DoCCaMK had the highest homology with Lilium longiflorum CCaMK. The DoCCaMK-GFP protein localized in the nucleus and cell membrane. CCaMK expression was significantly upregulated after symbiosis with Sebacina sp. KN-93 could be used as an inhibitor of CCaMK to inhibit D. officinale seed germination. Ca2+ influx and the concentration of the CCaMK in the SGS group was significantly more than that of the UGS group. The characterization of CCaMK provides certain genetic evidence for the involvement of this gene during seed germination and mycorrhizal cultivation in D. officinale.

Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid

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Perspective and challenges of mycorrhizal symbiosis in orchid medicinal plants

“…In recent years, various omics technologies, such as genome, transcriptome, proteome and metabolome, have been applied to elucidate the complexity of ORM interaction and provide a preliminary understanding of the establishment and signalling of mycorrhizal symbiosis in Orchidaceae. The common symbiotic signalling pathways (CSSP) involved in AM symbiosis are believed to exist in ORM ( Miura et al, 2018 , Xing et al, 2020 ). Plant roots exude hormone or flavoid compounds, and these compounds stimulate fungi to release signalling molecules; the two partners recognise each other through the receptor protein, promote successful fungal colonisation inside plant roots and further trigger the transcription regulation of mycorrhizal symbiosis-related genes ( Favre-Godal et al, 2020 ).…”

Armillaria mellea Symbiosis Drives Metabolomic and Transcriptomic Changes in Polyporus umbellatus Sclerotia

“…According to the transcriptome analysis, the DEGs of comp16933_c0, comp34626_c0 and comp32446_c0, respectively, encoding ERG6, ERG2 and ERG5 were selected for validating their expression patterns in the QR and the control groups. The PCR-amplification, the qRT-PCR protocol were all conducted using Real-time fluorescence quantitative PCR instrument (Roche, LightCycler 480), according to a previous study (Xing et al, 2013(Xing et al, , 2020.…”