Identification and expression of chemosensory receptor genes in the egg parasitoid Trissolcus basalis

Chen, Huayan; Lahey, Zachary; Talamas, Elijah J.; Johnson, Norman F.

doi:10.1016/j.cbd.2020.100758

Cited by 6 publications

(5 citation statements)

References 62 publications

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“…Gustatory receptors (Grs) and odorant receptors (Ors) are thought to be the most important chemosensory receptors. Like GPCRs, Ors, and Grs are seven-transmembrane domain receptors but belong to the chemosensory 7tm receptor superfamily (82,83). It has been suggested that the cause of gene expansion in GPCRs might be similar to that of chemosensory receptors, also to better adapt to the environment (32).…”

Section: Gpcrs Gene Expansion Occurred In a Lucorummentioning

confidence: 99%

Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

Gao

Wang

et al. 2021

Front. Endocrinol.

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G protein-coupled receptors (GPCRs) are the largest and most versatile family of transmembrane receptors in the cell and they play a vital role in the regulation of multiple physiological processes. The family Miridae (Hemiptera: Heteroptera) is one of the most diverse families of insects. Until now, information on GPCRs has been lacking in Miridae. Apolygus lucorum, a representative species of the Miridae, is an omnivorous pest that occurs worldwide and is notorious for causing serious damage to various crops and substantial economic losses. By searching the genome, 133 GPCRs were identified in A. lucorum. Compared with other model insects, we have observed GPCR genes to be remarkably expanded in A. lucorum, especially focusing on biogenic amine receptors and neuropeptide receptors. Among these, there is a novel large clade duplicated from known FMRFamide receptors (FMRFaRs). Moreover, the temporal and spatial expression profiles of the 133 genes across developmental stages were determined by transcriptome analysis. Most GPCR genes showed a low expression level in the whole organism of A. lucorum. However, there were a few highly expressed GPCR genes. The highly expressed LW opsins in the head probably relate to nocturning of A. lucorum, and the expression of Cirl at different times and in different tissues indicated it may be involved in growth and development of A. lucorum. We also found C2 leucine-rich repeat-containing GPCRs (LGRs) were mainly distributed in Hemiptera and Phthiraptera among insects. Our study was the first investigation on GPCRs in A. lucorum and it provided a molecular target for the regulation and control of Miridae pests.

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Section: Gpcrs Gene Expansion Occurred In a Lucorummentioning

confidence: 99%

Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

Gao

Wang

et al. 2021

Front. Endocrinol.

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“…kuwanae was less than that in N . vitripennis (301; Robertson et al., 2010) and Trissolcus basalis (171; Chen et al., 2021), but more than that in Propsilocerus akamusi (17; Yan et al., 2020). There is a huge variation in the number of OR genes between different species, probably due to the different reference databases, such as the transcriptome or genome.…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate chemosensory genes by antennal transcriptome analysis in an ectoparasitoid wasp

Chen

Liang

et al. 2021

J Applied Entomology

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Iseropus (Gregopimpla) kuwanae Vlereck (Hymenoptera: Ichneumonidae) is an ectoparasitoid wasp that attacks the larvae of Rondotia menciana Moore (Lepidoptera: Bombycidae), which is a major pest harming mulberry trees in China. In this study, we obtained the antennal transcriptome dataset of I. kuwanae using RNA‐Seq technology. A total of 22,090,144 clean reads were generated, and 21,802 unigenes were assembled. Among them, 11,546 (52.96%) unigenes were matched in the NCBI non‐redundant protein database (E‐value of 10−5). Moreover, a total of 9,229 (42.33%) and 5,305 (24.33%) unigenes were annotated by Gene Ontology and Clusters of Orthologous Groups of the proteins database respectively. By antennal transcriptome analysis, a total of 122 candidate chemosensory genes were identified, including 11 odorant‐binding protein (OBP) genes, 6 chemosensory protein (CSP) genes, 87 olfactory receptor (OR) genes (including one odorant co‐receptor, Orco), 16 ionotropic receptor genes and two sensory neuron membrane protein genes. RT‐qPCR assays revealed that seven of the 11 IkuwOBPs were predominantly expressed in the antennae of I. kuwanae adults. IkuwOBP4 was highly expressed in the head. IkuwOBP7 and IkuwOBP8 had higher expression levels in the legs. Interestingly, IkuwOBP6 was significantly expressed in both female antennae and ovipositor. The present findings can provide the basis for the subsequent in‐depth exploration of chemosensory mechanisms in I. kuwanae.

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“…Fifty million paired-end reads were sequenced (SRA accession number SRR15166997 ). The E. exitiosus metatranscriptome was assembled de novo using Trinity v.2.13.2 ( 4 ), following Chen et al ( 5 ). Open reading frames were determined using TransDecoder v.5.5.0 ( https://github.com/TransDecoder/TransDecoder ).…”

Section: Announcementmentioning

confidence: 99%

Genome Sequences of Novel Iflaviruses in the Gray Lawn Leafhopper, an Experimental Vector of Corn Stunt Spiroplasma

Andreason

Lahey

Ayala-Ortiz

et al. 2022

Microbiol Resour Announc

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Two novel iflaviruses were detected in the metatranscriptome of the gray lawn leafhopper, Exitianus exitiosus (Uhler). The assembled genome sequence of Exitianus exitiosus virus 1 was 9,858 nucleotides (nt) long and encodes a 3,083-amino acid (aa) polyprotein. Exitianus exitiosus virus 2 was 10,219 nt long and encodes a 2,947-aa polyprotein.

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Identification and expression of chemosensory receptor genes in the egg parasitoid Trissolcus basalis

Cited by 6 publications

References 62 publications

Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

Identification and Expression Analysis of G Protein-Coupled Receptors in the Miridae Insect Apolygus lucorum

Identification of candidate chemosensory genes by antennal transcriptome analysis in an ectoparasitoid wasp

Genome Sequences of Novel Iflaviruses in the Gray Lawn Leafhopper, an Experimental Vector of Corn Stunt Spiroplasma

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