Genome Sequences of Novel Iflaviruses in the Gray Lawn Leafhopper, an Experimental Vector of Corn Stunt Spiroplasma

Andreason, Sharon A.; Lahey, Zachary; Ayala-Ortiz, Christian; Simmons, Alvin M.

doi:10.1128/mra.00446-22

Microbiol Resour Announc

2022

DOI: 10.1128/mra.00446-22

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Genome Sequences of Novel Iflaviruses in the Gray Lawn Leafhopper, an Experimental Vector of Corn Stunt Spiroplasma

Sharon A. Andreason

Zachary Lahey

Christian Ayala-Ortiz

et al.

Abstract: Two novel iflaviruses were detected in the metatranscriptome of the gray lawn leafhopper, Exitianus exitiosus (Uhler). The assembled genome sequence of Exitianus exitiosus virus 1 was 9,858 nucleotides (nt) long and encodes a 3,083-amino acid (aa) polyprotein. Exitianus exitiosus virus 2 was 10,219 nt long and encodes a 2,947-aa polyprotein.

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Viromes of Tabanids from Russia

Litov,

Belova,

Kholodilov

et al. 2023

Viruses

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Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, the viromes of hematophagous invertebrates, such as mosquitoes and ixodid ticks, are being actively studied. Tabanidae (Diptera) are a widespread family, with members mostly known for their persistent hematophagous behavior. They transmit viral, bacterial, and other pathogens, both biologically and mechanically. However, tabanid viromes remain severely understudied. In this study, we used high-throughput sequencing to describe the viromes of several species in the Hybomitra, Tabanus, Chrysops, and Haematopota genera, which were collected in two distant parts of Russia: the Primorye Territory and Ryazan Region. We assembled fourteen full coding genomes of novel viruses, four partial coding genomes, as well as several fragmented viral sequences, which presumably belong to another twelve new viruses. All the discovered viruses were tested for their ability to replicate in mammalian porcine embryo kidney (PEK), tick HAE/CTVM8, and mosquito C6/36 cell lines. In total, 16 viruses were detected in at least one cell culture after three passages (for PEK and C6/36) or 3 weeks of persistence in HAE/CTVM8. However, in the majority of cases, qPCR showed a decline in virus load over time.

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Viromes of Tabanids from Russia

Litov,

Belova,

Kholodilov

et al. 2023

Viruses

View full text Add to dashboard Cite

show abstract

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Genome Sequences of Novel Iflaviruses in the Gray Lawn Leafhopper, an Experimental Vector of Corn Stunt Spiroplasma

Cited by 1 publication

References 8 publications

Viromes of Tabanids from Russia

Viromes of Tabanids from Russia

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