Identification and expression analysis of chitinase genes in Zizania latifolia in response to abiotic stress

Zhou, Niannian; An, Yulan; Gui, Zhicheng; Xu, Shuangshuang; He, Xin; Gao, Jie; Zeng, Donglin; Gan, Defang; Xu, Wenjuan

doi:10.1016/j.scienta.2019.108952

Cited by 17 publications

(13 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies have shown increments of endochitinase protein abundance during vegetative and flowering stages under drought stress in common bean ( Phaseolus vulgaris L.) 67 , similar to the accumulation of diverse endochitinase-like proteins (AUR62021381-RA, AUR62002379-RA, AUR62031322-RA, AUR62031316-RA, AUR62023809-RA) found in quinoa seeds harvested from rainfed conditions (Table 2 ). In addition, several GH19-like chitinases from Manchurian wild rice ( Zizania latifolia L.) increased their expression under abiotic stresses 68 and the accumulation of plant chitinases was found in roots of barley, corn, pea, soybean, and beans in response to heavy metal toxicity 69 . Other environmental stresses 70 also induced the accumulation of chitinases in agronomically important species such as Lycopersicon chilense 71 , bromegrass 72 , or blueberry 73 .…”

Section: Resultsmentioning

confidence: 99%

“…Underlined AUR codes show chitinase-related proteins exclusively identified in seeds harvested from rainfed conditions. www.nature.com/scientificreports/ upregulation of chitinase-related gene expression in many plants (including crops) when subjected to various abiotic stresses 29,64,[66][67][68][69][70][71][72][73]77 . Indeed, chitinases represent a huge family of proteins in plants, that include a great number of gene copies and evolutionary divergent sequences that have allowed them to acquire new functionalities resulting in emerging chitinase-like proteins (CLPs) that possess the ability to catalyse or bind different molecules other than chitin 33 .…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

See 1 more Smart Citation

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Poza-Viejo

Redondo‐Nieto

Matías

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Quinoa is an Andean crop whose cultivation has been extended to many different parts of the world in the last decade. It shows a great capacity for adaptation to diverse climate conditions, including environmental stressors, and, moreover, the seeds are very nutritious in part due to their high protein content, which is rich in essential amino acids. They are gluten-free seeds and contain good amounts of other nutrients such as unsaturated fatty acids, vitamins, or minerals. Also, the use of quinoa hydrolysates and peptides has been linked to numerous health benefits. Altogether, these aspects have situated quinoa as a crop able to contribute to food security worldwide. Aiming to deepen our understanding of the protein quality and function of quinoa seeds and how they can vary when this crop is subjected to water-limiting conditions, a shotgun proteomics analysis was performed to obtain the proteomes of quinoa seeds harvested from two different water regimes in the field: rainfed and irrigated conditions. Differentially increased levels of proteins determined in seeds from each field condition were analysed, and the enrichment of chitinase-related proteins in seeds harvested from rainfed conditions was found. These proteins are described as pathogen-related proteins and can be accumulated under abiotic stress. Thus, our findings suggest that chitinase-like proteins in quinoa seeds can be potential biomarkers of drought. Also, this study points to the need for further research to unveil their role in conferring tolerance when coping with water-deficient conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Poza-Viejo

Redondo‐Nieto

Matías

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Other studies have shown increments of endochitinase protein abundance during vegetative and flowering stages under drought stress in common bean (Phaseolus vulgaris L.) (Gupta et al , 2019), similar to the accumulation of diverse endochitinase-like proteins (AUR62021381-RA, AUR62002379-RA, AUR62031322-RA, AUR62031316-RA, AUR62023809-RA) found in quinoa seeds harvested from rainfed conditions (Table 2). In addition, several GH19-like chitinases from Manchurian wild rice (Zizania latifolia L.) increased their expression under abiotic stresses (Zhou et al , 2020) and the accumulation of plant chitinases was found in roots of barley, corn, pea, soybean, and beans in response to heavy metal toxicity (Bekesiova et al , 2008). Other environmental stresses also induced the accumulation of chitinases in agronomically important species such as tomato, bromegrass, or blueberry (Ernst et al ., 1992;Chen et al , 1994;Nakamura et al , 2008;Kikuchi and Masuda, 2009).…”

Section: Chitinase-related Proteins Were Accumulated In Quinoa Seeds ...mentioning

confidence: 99%

“…These proteins are well characterized pathogenesis-related (PR) proteins that act degrading chitin in different organisms including plants, animals, or bacteria (Grover, 2012). Nonetheless, previous works have shown an induced chitinase activity or the upregulation of chitinase-related gene expression in many plants (including crops) when subjected to various abiotic stresses (Ernst et al , 1992;Chen et al , 1994;Hong and Hwang, 2006;Bekesiova et al , 2008;Nakamura et al , 2008;Kikuchi and Masuda, 2009;Grover, 2012;Rasheed et al , 2016;Gupta et al, 2019;Zhou et al , 2020). Indeed, chitinases represent a huge family of proteins in plants, that include a great number of gene copies and evolutionary divergent sequences that have allowed them to acquire new functionalities resulting in emerging chitinase-like proteins (CLPs) that possess the ability to catalyse or bind different molecules other than chitin (Kesari et al , 2015).…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Reguera

Poza-Viejo

Redondo‐Nieto

et al. 2022

Preprint

View full text Add to dashboard Cite

Quinoa is an Andean crop whose cultivation has been extended to many different parts of the world in the last decade. It shows a great capacity for adaptation to diverse climate conditions, including environmental stressors, and moreover, the seeds are very nutritious in part due to their high protein content which is rich in essential amino acids. They also contain good amounts of other nutrients such as unsaturated fatty acids, vitamins, or minerals and are gluten-free seeds. Also, the use of quinoa hydrolysates and peptides has been linked to numerous health benefits. Altogether, these aspects have situated quinoa as a crop able to contribute to food security worldwide. Aiming to deepen our understanding of the protein quality and function of quinoa seeds and how they can vary when this crop is subjected to water-limiting conditions, a shotgun proteomics analysis was performed to obtain the proteomes of quinoa seeds harvested from two different water regimes in field: rainfed and irrigated conditions. Overrepresented proteins in seeds from each field condition were analysed, and the enrichment of chitinase-related proteins in seeds harvested from rainfed conditions was found. These proteins are described as pathogen-related proteins and can be accumulated under abiotic stress. Thus, our findings suggest that chitinase-like proteins in quinoa seeds can be potential biomarkers of drought. Also, this study points to the need for further research to unveil their role in conferring tolerance when coping with water-deficient conditions.

show abstract

“…esculenta interaction, we established an in vitro regeneration system for Z. latifolia, and carried out inoculation experiments with U. esculenta (Yang, 2018). We also studied the physiological responses of male Z. latifolia to U. esculenta infection, and found that expression of most enzymes was significantly different at 3 hr or 12 hr post-infection, suggesting that responses of Z. latifolia to U. esculenta infection mainly occurred in the early stages of the colonisation process (Zhou, 2021). However, the mechanism underpinning the interaction between U. esculenta and Z. latifolia that leads to enlargement of swollen stem galls remains unclear.…”

Section: Introductionmentioning

confidence: 99%

MAP kinase and plant–pathogen interactions govern male Zizania latifolia responses to Ustilago esculenta during the early stages of infection

Guo

Zhou

Bai

et al. 2023

Folia Horticulturae

Self Cite

View full text Add to dashboard Cite

This study explored the effect of Ustilago esculenta infection on differentially expressed genes (DEGs) in male Manchurian wild rice (Zizania latifolia). We injected male Jiaobai plants at the five-leaf stage with U. esculenta suspension, and leaves at different timepoints after inoculation were collected for transcriptome sequencing. Transcriptome sequencing identified 1,226 DEGs mainly enriched in resistance-related processes. Weighted gene coexpression network analysis (WGCNA) showed that ‘plant–pathogen interaction’ and ‘MAPK signalling pathway-plant’ were the two most important upregulated pathways, and 16 candidate genes related to response to U. esculenta infection were screened. The results provide a basis for determining the molecular mechanisms of the response of male Z. latifolia to U. esculenta infection.

show abstract

Identification and expression analysis of chitinase genes in Zizania latifolia in response to abiotic stress

Cited by 17 publications

References 48 publications

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

Shotgun proteomics of quinoa seeds reveals chitinases enrichment under rainfed conditions

MAP kinase and plant–pathogen interactions govern male Zizania latifolia responses to Ustilago esculenta during the early stages of infection

Contact Info

Product

Resources

About