Identification and expression analysis of OsHsfs in rice

Wang, Chuang; Zhang, Qian; Shou, Huixia

doi:10.1631/jzus.b0820190

Cited by 55 publications

(47 citation statements)

References 30 publications

(48 reference statements)

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“…Until now, only one HSFA2 has been identified in tomato and Arabidopsis (Scharf et al 1990;Nover et al 2001;Baniwal et al 2004;Kotak et al 2004;von Koskull-Doring et al 2007), whereas six OsH-SFA2 members have been identified in rice, with OsHSFA2a, OsHSFA2c and OsHSFA2d responding to HS (Baniwal et al 2004;Xiong et al 2005;von Koskull-Doring et al 2007;Gao et al 2008;Mittal et al 2009;Wang et al 2009). Therefore, we speculate that the redundant functions of OsHSFA2a, OsHSFA2c and OsHSFA2d might promote functional diversity via an alternative splicing pattern that alters the protein subcellular localisation under HS.…”

Section: Discussionmentioning

confidence: 99%

An alternatively spliced heat shock transcription factor, OsHSFA2dI, functions in the heat stress‐induced unfolded protein response in rice

et al. 2015

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As sessile organisms, plants have evolved a wide range of defence pathways to cope with environmental stress such as heat shock. However, the molecular mechanism of these defence pathways remains unclear in rice. In this study, we found that OsHSFA2d, a heat shock transcriptional factor, encodes two main splice variant proteins, OsHSFA2dI and OsHSFA2dII in rice. Under normal conditions, OsHSFA2dII is the dominant but transcriptionally inactive spliced form. However, when the plant suffers heat stress, OsHSFA2d is alternatively spliced into a transcriptionally active form, OsHSFA2dI, which participates in the heat stress response (HSR). Further study found that this alternative splicing was induced by heat shock rather than photoperiod. We found that OsHSFA2dI is localised to the nucleus, whereas OsHSFA2dII is localised to the nucleus and cytoplasm. Moreover, expression of the unfolded protein response (UNFOLDED PROTEIN RESPONSE) sensors, OsIRE1, OsbZIP39/OsbZIP60 and the UNFOLDED PROTEIN RESPONSE marker OsBiP1, was up-regulated. Interestingly, OsbZIP50 was also alternatively spliced under heat stress, indicating that UNFOLDED PROTEIN RESPONSE signalling pathways were activated by heat stress to re-establish cellular protein homeostasis. We further demonstrated that OsHSFA2dI participated in the unfolded protein response by regulating expression of OsBiP1.

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Section: Discussionmentioning

confidence: 99%

An alternatively spliced heat shock transcription factor, OsHSFA2dI, functions in the heat stress‐induced unfolded protein response in rice

et al. 2015

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“…1976). Rice plants were grown in growth chambers with a 12‐h photoperiod (200 μmol photons m −2 ·s −1 ) and a day/night temperature of 30/22 °C after germination, as previously reported (Wang et al. 2009b).…”

Section: Methodsmentioning

confidence: 99%

Identification of rice purple acid phosphatases related to posphate starvation signalling

et al. 2010

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Purple acid phosphatases (PAPs) are a family of metallo-phosphoesterases involved in a variety of physiological functions, especially phosphate deficiency adaptations in plants. We identified 26 putative PAP genes by a genome-wide analysis of rice (Oryza sativa), 24 of which have isolated EST sequences in the dbEST database. Amino acid sequence analysis revealed that 25 of these genes possess sets of metal-ligating residues typical of known PAPs. Phylogenetic analysis classified the 26 rice and 29 Arabidopsis PAPs into three main groups and seven subgroups. We detected transcripts of 21 PAP genes in roots or leaves of rice seedlings. The expression levels of ten PAP genes were up-regulated by both phosphate deprivation and over-expression of the transcription factor OsPHR2. These PAP genes all contained one or two OsPHR2 binding elements in their promoter regions, implying that they are directly regulated by OsPHR2. Both acid phosphatase (AP) and surface secretory acid phosphatase (SAP) activity assays showed that the up-regulation of PAPs by Pi starvation, OsPHR2 over-expression, PHO2 knockout or OsSPX1 RNA interference led to an increase in AP and SAP activity in rice roots. This study reveals the potential for developing technologies for crop improvement in phosphorus use efficiency.

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“…A thermotolerant line encoding the rice the heat stress transcription factor OsHsfA2 fell out of the analysis as highly expressing several classes of heat-shock proteins (Yokotani et al, 2008), and also displaying tolerance to high-salinity stress. A genome-wide analysis of rice, including Oryza sativa L. ssp japonica and Oryza sativa L. ssp indica (Wang et al, 2009), identified 25 rice Hsf genes. Promoter analysis identified a number of stress-related ciselements in the promoter regions, however no correlation was found between heat-shock gene responses and their cis-elements.…”

Section: Heat Stressmentioning

confidence: 99%

“…A recent study in the dicot grape (Vitis vinifera) identified four genes strongly upregulated by heat stress, whose overexpression resulted in the acquisition of thermotolerance in Arabidopsis (Kobayashi et al, 2010). Further in vivo studies in grape are underway to elucidate chaperone mechanisms, localization, and functions under stress conditions (Wang et al, 2009). The complete mechanisms of Hsp mediated thermotolerance remains to be fully elucidated in plants; however work with transgenics has shown that altered levels of Hsps and Hsfs have dramatic effects on plants resistance to elevated temperatures.…”

Section: Heat Stressmentioning

confidence: 99%