Identification and expression analysis of differentially expressed genes from shrimp (Penaeus monodon) in response to low salinity stress

Profiling of Up-Regulated Genes Response to Acute Hypo-Osmotic Stress in Hepatopancreas and Gill of the Pacific White Shrimps (Litopenaeus vannamei)

“…These results are similar to the report by Shekhar (Shekhar et al, 2013) Numerous genes detected in the SSH library, such as Adapter molecule Crk, putative, chitinase precursor, nuclear progesterone receptor, estrogen sulfotransferase, and destabilase I, are involved in signal transduction and other biological progress. The increased expression of these genes in shrimps with hypoosmotic stress indicated that signal transductions and other biological progresses are involved in osmotic physiological adaption.…”

“…Adisak et al, obtained 185 transcripts and uncovered 8 novel YHV-responsive genes from haemocyte libraries of P. monodon induced by yellow head virus after 24h and 48/72h (Prapavorarat, Pongsomboon, & Tassanakajon, 2010). Four suppression subtractive hybridization (SSH) cDNA libraries were constructed to identify differentially expressed salinity stress responsive genes of black tiger shrimp, Penaeus monodon exposed to low (3 ppt) salinity and high (50 ppt) salinity conditions (Shekhar et al, 2013;Shekhar et al, 2014). We also performed uppression subtractive hybridization (SSH) in juvenile L. vannamei exposed to long-term low salinity (2 ppt) for 56 days (Gao et al, 2012).…”

“…Employing SSH, Foil et al, (Fiol, Chan, & Kültz, 2006) discovered 20 genes cluster together in six molecular processes that are rapidly activated in Tilapia gills upon salinity transfer; James et al, (James, Thampuran, Lalitha, Rajan, & Joseph, 2010) identified 17 novel proteins which invovled in immunity in hepatopancreas of Fenneropenaeus; Pan et al, (D. Pan, He, Yang, Liu, & Xu, 2005) found 8 novel genes related to virus-resistant in hepatopancreas of panaeid shrimps. Regarding osmoregulation of shrimps, Shekhar et al,identified differentially expressed genes in response to salinity stress with suppression subtractive hybridization (SSH) through cDNA library constructed from gill and gut tissues of the giant tiger shrimps (Penaeus monodon) exposed to low (Shekhar, Kiruthika, & Ponniah, 2013) and high (Shekhar, Kiruthika, Rajesh, & Ponniah, 2014) salinity conditions. The resulted expressed sequence tags (EST) provided a partial description of the transcribed regions or activities of a genome in a tissue or an organism under specific experimental conditions.…”

Characterization and function analysis of Hsp60 and Hsp10 under different acute stresses in black tiger shrimp, Penaeus monodon

“…8). The two tissues have similarly been reported to be environmentally sensitive tissues in other species Li et al 2012;Shekhar et al 2013). Therefore, PmHsp60 and PmHsp10 transcript levels in the hepatopancreas and gills were investigated upon exposure of P. monodon to different environmental stresses, including pH challenge, osmotic stress, and heavy metal exposure.…”

“…T cells in experimentally induced salt-driven hypertension present a CD4 clonal response to Hsp70, which is overexpressed in the kidneys (Pons et al 2013). Hsp21 was identified as a differentially expressed gene from a forward SSH cDNA library constructed from gut tissues of P. monodon showing significant increases in gene expression under osmotic stress (Shekhar et al 2013). Ion uptake in crustaceans is mostly attributed to Na + /K + -ATPase and Ca 2+ -ATPase activity (Meyran and Graf 1986;Lima et al 1997;Furriel et al 2004;Masui et al 2005).…”