Identification and expression analysis of phosphate transporter genes and metabolites in response to phosphate stress in Capsicum annuum

Integrated analysis of DNA methylation, transcriptome, and global metabolites in interspecific heterotic Capsicum F1 hybrid

“…Equally, ∼1 μg of total RNA was used to synthesize cDNA using PrimeScript IV 1 st strand cDNA Synthesis Mix (Takara). The real-time PCR reaction was set up using TB Green Premix Ex Taq II Master Mix (Clontech, USA) and run on the Bio-Rad CFX96 Real-Time System (Bio-Rad, USA) using the thermal profile of initial denaturation at 95°C for 30 s, followed by amplification step with 40 cycles of 95°C for 5 s and 60°C for 30 s. Then final amplification was completed at 65°C for 5 s, as described earlier ( Dubey et al., 2019 ; Ahmad et al., 2021b ). Further, qRT-PCR expression was compared with in silico RNAseq expression data.…”

“…in increasing food shelf-life, improving the sensory properties, seasoning, and coloring of foods, in cosmetic and pharma industry ( Baenas et al., 2019 ). Several studies reported the presence of intra and interspecific diversity of fruit traits, metabolites content, and other agronomic traits in Capsicum species ( Ahmad et al., 2021a , 2021b ; Araceli et al., 2009 ; Chhapekar et al., 2020 ; Liu et al., 2020 ; Sarpras et al, 2016 ; Sarpras et al., 2019 ). Furthermore, many studies on marker development, QTL/gene(s) mapping of economically important traits, whole transcriptome, and genome sequencing have been reported on Capsicum species ( Ahn et al., 2014 ; Chhapekar et al., 2021 ; Dubey et al., 2019 ; Jaiswal et al., 2020 ; Kim et al., 2020 ; Liu et al., 2019 ; Martínez et al., 2021 ; Razo-Mendivil et al., 2021 ).…”

Integrated omics analysis identified genes and their splice variants involved in fruit development and metabolites production in Capsicum species

“…The global metabolome of EG, MG and Br fruit developmental stages from both Cann and Cfrut (each stage with two biological replicates), was analyzed using gas chromatography coupled with mass spectrometry (GC-MS) following the method described by Lisec et al (2015) with slight modi cation (Ahmad et al, 2021;Lisec et al, 2006). Brie y, 100 mg of fruit sample from each individual fruit stage was homogenized with mortar and pestle using liquid nitrogen and then 1400 µl of pure methanol (chilled) was added.…”

“…Finally, the supernatant of each sample was dried in a vacuum concentrator without heating. For derivatization, 40µl of methoxyamine hydrochloride (20 mg ml -1 ) in pyridine was added in all samples, vortexed and kept in a shaker for 2 h at 37 o C. For trimethylsilylation, 70 μl of N-Methyl-N-(trimethylsilyl) tri uoroacetamide (MSTFA) as a silylating agent was added and then vortexed and incubated with shaking for 30 min at 37 o C. The detected metabolites were quanti ed using GCMS post run analysis program (Shimadzu Scienti c Instruments) following criteria described by Ahmad et al (2021). Pathway enrichment of metabolites were analyzed using MetaboAnalystR (Pang et al, 2021) and pathways number of metabolites >3 and pathway impact >0.2 along with p-value <0.01 were considered as signi cantly enriched.…”

“…in increasing food shelf-life, improve the sensory properties and seasoning of foods, and coloring food, in cosmetic and pharma industry (Baenas et al, 2019). A wide-range of physiological and morphological changes including shifts in transcriptional expression and biosynthesis of several metabolites take place during Capsicum fruit development (Ahmad et al, 2021;Sarpras et al, 2019). For instance, during course of Capsicum fruit development, the differential expression of several genes such as Psy, PDS, CA1, Ccs, NCED3, SGR1, CaERF66, CaERF82, CaERF97, CaERF107, CaDIV1, CaMYB3R-1, CabHLH09, CabHLH032, CabHLH095, and CabHLH100 are directly or indirectly involved in regulation of carotenoid biosynthesis (Berry et In plants, expression of genes is regulated through various post-transcriptional mechanisms (PTM), which in turn regulate/affect the diverse developmental or physiological processes (Floris et al, 2009; X.…”