Identification and distribution of some developmental isoforms of myosin heavy chains in avian muscle fibres

Kilby, Karen; Dhoot, Gurtej K.

doi:10.1007/bf01738756

Cited by 23 publications

(10 citation statements)

References 22 publications

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“…However, a similar pattern of differentiation occurs in the chick, albeit with the existence of more isoforms of slow myosin than have yet been found in mammals (Crow andStockdale, 1986: Kilby andDhoot, 1988;Sweeney. Kennedy, Zak, Kokjohn, and Kelley.…”

Section: The Origin Of Fiber Diversity In Skeletal Musclesmentioning

confidence: 77%

The origin and selective innervation of early muscle fiber types in the rat

1990

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The diversity of muscle fiber types present in adult animals is present also in the fetus. Fibers generated early and late in fetal development undergo a stereotyped sequence of myosin expressions in giving rise to these fiber types. The differentiation of these fetal fiber types does not require innervation. However, evidence obtained from experiments identifying the types of fibers innervated by single motors suggests that the nervous system comes to recognize this diversity, at least during early postnatal life. Reinnervation experiments suggest that this recognition can occur in the absence of the timing cues normally present in the genesis of fiber types. Thus, a selective innervation of muscle fiber types occurs during development. The role of rearrangement of initial synaptic connections in generating this selectivity is discussed.

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Section: The Origin Of Fiber Diversity In Skeletal Musclesmentioning

confidence: 77%

The origin and selective innervation of early muscle fiber types in the rat

1990

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“…In addition, MAb 9812 [19] and MAb F262Dll (Sera-Lab), specilic to slow twitch MHCI (B-cardiac MHC), and the polyclonal antibody NN5, specific to human fetal MHC [20], were used.…”

Section: Zmmunocytochemistrymentioning

confidence: 99%

Electrophoretically defined myosin heavy chain patterns of single human muscle spindles

et al. 1993

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At least four myosin heavy chain (MHC) isoforms were separated by SDS-PAGE in extracts of intrafusal fibers isolated by microdissection from human lumbrical muscles. The fastest migrating MHC represents a slow isoform. The slowest migrating MHC was identified as the embryonic MHC,,. A faint band, moving slightly faster than MHC,,,, most likely represents a neonatal/fetal MHC isoform. A prominent band, moving between the latter and the slow isoform is suggested to represent a hitherto unidentified, spindle-specific MHC isoform, MHC,, Human muscle; Intrafusal fiber; Muscle spindle; Myosin heavy chain isoform; Single fiber study

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“…The proteins were transferred from the gel to nitrocellulose sheets (0.45 μm, Schleicher & Schuell, Dassel, FRG) by the method of Towbin et al [33]. The blotted proteins were reacted with three different monoclonal antibodies against specific MHC isoforms: antiembryonic 2B6 [7], anti-slow 96J [16], and anti-fast MY32 (Sigma, München, FRG). Production and characterization of these antibodies have been described elsewhere [7,16].…”

Section: Immunoblottingmentioning

confidence: 99%

“…The blotted proteins were reacted with three different monoclonal antibodies against specific MHC isoforms: antiembryonic 2B6 [7], anti-slow 96J [16], and anti-fast MY32 (Sigma, München, FRG). Production and characterization of these antibodies have been described elsewhere [7,16]. Antibody 96J was used at 1:1000 dilution, antibody MY32 at 1:500 dilution.…”

Section: Immunoblottingmentioning

confidence: 99%

Characterization of myosin isoforms in satellite cell cultures from adult rat diaphragm, soleus and tibialis anterior muscles

1990

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Satellite cells were isolated by enzymatic dissociation and Percoll gradient centrifugation from adult rat diaphragm, soleus, and tibialis anterior muscles with fairly reproducible yields. Diaphragm and soleus muscle yielded approximately five times more satellite cells than tibialis anterior muscle. According to light microscopic criteria, no morphological differences existed between the satellite cell cultures of different origin. Contrary to the donor muscles, myotubes from the 10-day-cultured satellite cells contained a uniform myosin heavy chain (MHC) pattern with predominance of an immunochemically identified embryonic heavy chain. The three types of cultures displayed a typical embryonic light chain (LC) pattern with LC1 emb , LC1f, LC2f, and traces of LC3f. The isomyosin pattern was characterized by four embryonic isomyosins, eMleM4, with similar distributions in the three cultures. In summary, these myosin analyses provide no evidence for the existence of satellite cell diversity among three rat muscles of different fibertype composition, at least not under the applied in vitro conditions.

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Identification and distribution of some developmental isoforms of myosin heavy chains in avian muscle fibres

Cited by 23 publications

References 22 publications

The origin and selective innervation of early muscle fiber types in the rat

The origin and selective innervation of early muscle fiber types in the rat

Electrophoretically defined myosin heavy chain patterns of single human muscle spindles

Characterization of myosin isoforms in satellite cell cultures from adult rat diaphragm, soleus and tibialis anterior muscles

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