Characterization of myosin isoforms in satellite cell cultures from adult rat diaphragm, soleus and tibialis anterior muscles

Düsterhöft, Sabine; Yablonka–Reuveni, Zipora; Pette, Dirk

doi:10.1111/j.1432-0436.1990.tb00472.x

Cited by 50 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conflicting evidence exists as to whether muscle-specific satellite cells are pre-programmed to some extent, although it has been suggested that the proportions of satellite cells derived from primary and secondary myoblasts may differ between muscles. 31 Some studies describe no differences in the expression of contractile proteins in cultured satellite cells isolated from slow postural or fast muscles, 9 while others show that fast muscle derived cells appear to have low or no expression of type I MHC following extensive electrical stimulation. 8,10,32 These studies have all been carried out in small animals, focussing exclusively on the ability to develop different phenotypes within differentiated myotubes.…”

Section: Discussionmentioning

confidence: 99%

“…Some suggest that expression of the contractile proteins in myotubes differ, while others do not. [8][9][10] To date, the majority of studies have used cells isolated and purified from one preferred tissue type or pooled from a number of different muscles. While satellite cell populations are known to be heterogeneous with regard to their proliferation, rarely been considered pertinent to their differentiation capabilities.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Muscle origin of porcine satellite cells affects in vitro differentiation potential

Redshaw¹,

McOrist²,

Loughna³

2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

Post-natal muscle regeneration relies on the activation of tissue stem cells known as satellite cells, to repair damage following exercise trauma and disease. Satellite cells from individual muscles are known to be heterogeneous with regard to proliferation, fusion and transplantation abilities, although the muscle origin has rarely been considered pertinent to their differentiation capabilities. In this study we compared the potential of two functionally distinct skeletal muscle satellite cell populations from porcine diaphragm and hind-limb semi-membranosus muscles. These two muscles were chosen primarily for differences in metabolic and contractile properties: the diaphragm is more continuously active and has a greater oxidative capacity. Cells were induced to differentiate towards myogenic and adipogenic lineages, and here we have shown that cells from diaphragm exhibit a significantly greater degree of myogenesis compared with those from semi-membranosus, while the converse was true for adipogenesis. Unexpectedly, both conditions generated small numbers of cells with neuronal characteristics for both muscle types, although more so in cells derived from the diaphragm. With increased interest in muscle adiposity with age and disease, these findings suggest that muscle origin of satellite cells does affect lineage fate, however whether differences in developmental origin or metabolic activity of the parent tissue govern this, remains to be determined.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Muscle origin of porcine satellite cells affects in vitro differentiation potential

Redshaw¹,

McOrist²,

Loughna³

2010

Cell Biochemistry & Function

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 79%

“…This assumption has been made on the basis of the expression of protein markers that are thought to represent the phenotype of muscle, such as myosin heavy chain (MHC), myosin light chain (MLC), and myogenic regulatory factors (see Table 1). However, depending on the protein analyzed, the culture conditions, and the organism studied, this difference has not always been observed (3,4,(12)(13)(14)30).Regardless of whether there are differences in the expression of a single protein, the rate of contraction and relaxation of muscle is the result of numerous protein systems, Ca 2ϩ release, regulatory proteins (MLC, troponins, and tropomyosin), MHC, and Ca 2ϩ sequestering, all working together to produce a faster muscle. Therefore, analyzing a single protein or even representative proteins could produce erroneous conclusions.…”

mentioning

confidence: 88%

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Huang¹,

Dennis²,

Baar³

2006

American Journal of Physiology-Cell Physiology

View full text Add to dashboard Cite

In vitro studies have used protein markers to distinguish between myogenic cells isolated from fast and slow skeletal muscles. The protein markers provide some support for the hypothesis that satellite cells from fast and slow muscles are different, but the data are equivocal. To test this hypothesis directly, three-dimensional skeletal muscle constructs were engineered from myogenic cells isolated from fast tibialis anterior (TA) and slow soleus (SOL) muscles of rats and functionality was tested. Time to peak twitch tension (TPT) and half relaxation time (RT(1/2)) were approximately 30% slower in constructs from the SOL. The slower contraction and relaxation times for the SOL constructs resulted in left shift of the force-frequency curve compared with those from the TA. Western blot analysis showed a 60% greater quantity of fast myosin heavy chain in the TA constructs. 14 days of chronic low-frequency electrical stimulation resulted in a 15% slower TPT and a 14% slower RT(1/2), but no change in absolute force production in the TA constructs. In SOL constructs, slow electrical stimulation resulted in an 80% increase in absolute force production with no change in TPT or RT(1/2). The addition of cyclosporine A did not prevent the increase in force in SOL constructs after chronic low-frequency electrical stimulation, suggesting that calcineurin is not responsible for the increase in force. We conclude that myogenic cells associated with a slow muscle are imprinted to produce muscle that contracts and relaxes slowly and that calcineurin activity cannot explain the response to a slow pattern of electrical stimulation.

show abstract

“…A second and more likely interpretation for the time course of the adaptations in the embryonic MHC composition is that the compensatory hypertrophy after overload is associated with an increase in the number of myonuclei to maintain a relatively constant nuclear domain (cytoplasmic volume per myonucleus). This process would involve satellite cell activation (1), cells that initially express embryonic MHC after fusion with either existing fibers or other satellite cells (9). After 10 wk of overload when the hypertrophic response has plateaued, these cells would most likely have switched to adult MHC isoform expression.…”

Section: Mhc Plasticity In Functionally Overloaded Fibersmentioning

confidence: 99%

Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers

Roy

Talmadge

Fox

et al. 1997

Journal of Applied Physiology

View full text Add to dashboard Cite

Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers. J. Appl. Physiol. 83(1): 280-290, 1997.-The effects of 1 and 10 wk of functional overload (FO) of the rat plantaris with (FO Tr ) and without daily endurance treadmill training on its myosin heavy chain (MHC) composition were studied. After 1 and 10 wk of FO, plantaris mass was 22 and 56% greater in FO and 37 and 94% greater, respectively, in FO Tr rats compared with agematched controls. At 1 wk, pure type I and pure type IIa MHC fibers were hypertrophied in FO (39 and 44%) and FO Tr (70 and 87%) rats. By 10 wk all fiber types comprising .5% of the fibers sampled showed a hypertrophic response in both FO groups. One week of FO increased the percentage of hybrid (containing both type I and type IIa MHC) fibers and of fibers containing embryonic MHC. By 10 wk, the percentage of pure type I MHC fibers was ,40% in both FO groups compared with 15% in controls, and the percentage of fibers containing embryonic MHC was similar to that in controls. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses showed an increase in type I MHC and a decrease in type IIb MHC in both FO groups at 10 wk, whereas little change was observed at 1 wk. These data are consistent with hypertrophy and transformation from faster to slower MHC isoforms in chronically overloaded muscles. The additional overload imposed by daily endurance treadmill training employed in this study (1.6 km/day; 10% incline) results in a larger hypertrophic response but appears to have a minimal effect on the MHC adaptations.immunohistochemistry; gel electrophoresis; hypertrophy; fiber type conversion; fiber size FUNCTIONAL OVERLOAD (FO) of the rat plantaris by removal of its major synergists results in hypertrophy and a shift in the contractile, biochemical, and metabolic properties toward those observed in a ''slower'' muscle (26). The extent of these adaptations may be related, in part, to the activity level of the rats after surgery. For example, it appears that the amount of hypertrophy is greater in rats that are exercised on a treadmill than in rats not exercised after FO (13,25), although this is somewhat controversial (3). Thus one of the mechanisms regulating muscle hypertrophy may be the amount and/or type of activity that the overloaded muscle experiences after ablation of its synergists. This contention is supported by the observation that in cats, a relatively sedentary animal, FO of the plantaris has little effect on the mass of the muscle unless the cat is exercised. In addition, the largest amount of hypertrophy was found when the cats were subjected to high-intensity exercise, i.e., sprinting and jumping (26,29).On the basis of gel electrophoresis analyses, FO of the rat plantaris has been shown to result in increases in the percent composition of type I, IIa, and IIx and a decrease in type IIb myosin heavy chains (MHCs) (8,10,19,23,37,38). The effects of exercise on the degree of conversion from fast to slow fibers in FO muscles, however, are e...

show abstract

Characterization of myosin isoforms in satellite cell cultures from adult rat diaphragm, soleus and tibialis anterior muscles

Cited by 50 publications

References 36 publications

Muscle origin of porcine satellite cells affects in vitro differentiation potential

Muscle origin of porcine satellite cells affects in vitro differentiation potential

Cultured slow vs. fast skeletal muscle cells differ in physiology and responsiveness to stimulation

Modulation of MHC isoforms in functionally overloaded and exercised rat plantaris fibers

Contact Info

Product

Resources

About