Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR

Rezavand, Babak; Poornaki, Abbas Mahmoodzadeh; Mokhtari, Keahan; Mohammad, Alireza; Andalibian, Ammar; Abdi, Jahangir

doi:10.1016/s2222-1808(15)61044-1

Cited by 10 publications

(15 citation statements)

References 15 publications

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“…This result is the same as that of Ocak et al (17) who studied hemodialysis patients and healthy volunteers in Turkey. On the other hand, Aufy et al (21), Sahinl et al (16), Maraghi et al (18), Ebrahim Zadeh et al (19) , Rezavand et al (20) and Nissaptorn et al (23) studies determined 16.7%, 6.8% , 8.6 %, 13.5%, 3.3% and 5.7% respectively in the patient groups, which is not compatible with our results.…”

Section: Discussioncontrasting

confidence: 88%

“…The results of the patient group share a similar frequency with figures reported in Turkey (76.5%) (17). However, the data in our study were higher than those reported by Sahinl et al (16) (59.1%) and Maraghi et al (18), Ebrahim Zadeh et al (19) and Rezavand et al (20) of 59.1% ,40.67% , 56.7% and 60%, respectively in Iran, and Aufy et al (21) and Alvarado-Esquivel et al (22) of 56.06%, 56.7% and 10%, respectively in Turkey, Egypt and Mexico; whereas in our results the percentage of Anti-Toxoplasma gondii IgG antibody in the volunteer group was more than other studies (17-22). Furthermore, in our study, there were no positive cases of anti- Toxoplasma gondii IgM antibody in the patient group and only 2.76% of the healthy volunteer showed the IgM antibody.…”

Section: Discussioncontrasting

confidence: 78%

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Evaluation of Anti-Toxoplasma gondii Antibodies in Hemodialysis Patients with Chronic Kidney Disease in Sari, Iran

et al. 2016

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BackgroundToxoplasma gondii has worldwide distribution and is one of the most prevalent infectious agents in humans.ObjectivesThe aim of this study was to determine the prevalence of anti-Toxoplasma gondii antibodies in hemodialysis patients with chronic kidney disease (CKD) in the hemodialysis unit of Fatemeh Zahra hospital at the Mazandaran University of Medical Sciences in Sari, Iran.MethodsSeventy-three patients with CKD and 145 healthy volunteers were assessed for anti-Toxoplasma gondii (IgG, IgM, and IgA) antibodies using a conventional ELISA technique.ResultsThe anti-Toxoplasma gondii IgG antibody was detected in 80.8% of the cases in the patient group, while 31.5% of the cases in the CKD patient group and 31.8% in the control group were found to be positive for the anti-Toxoplasma gondii IgA antibody. All the patients in the CKD group were negative for the anti-Toxoplasma gondii IgM antibody, although 2.76% of the healthy volunteers were found to be positive. The present study suggests that there was no significant difference between the hemodialysis patient group and the healthy volunteers.ConclusionsThe epidemiological data collected in this study could serve as a reference for future studies and may be useful in developing preventive and educational strategies, and consequently reducing healthcare expenditure.

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Section: Discussioncontrasting

confidence: 88%

Section: Discussioncontrasting

confidence: 78%

Evaluation of Anti-Toxoplasma gondii Antibodies in Hemodialysis Patients with Chronic Kidney Disease in Sari, Iran

et al. 2016

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“…17 In comparison with other studies, the seropositivity rate of Toxoplasma in the current study was lower than studies conducted on hemodialysis patients in Mazandaran (80%), Tehran (60%), and Fars Province (59.1%); moreover seroprevalence of T. gondii in our study was also lower than two serological studies on hemodialysis patients in other countries including Turkey (76.5%) and Malaysia (51%). [18][19][20][21][22] In a systematic review study conducted by Ahmadpour et al (2014) to examine the overall seroprevalence rate of toxoplasmosis in cancer patients, 45.06%% was positive, moreover in a systematic review and meta-analysis in China, seroprevalence of T. gondii in cancer patients was found to be 20.59% compared to 6.31% in healthy individuals. 11,23 In the study performed by Hassanzadeh et al (2017), 60.3% and 6% of individuals with immunodeficiency were positive for IgG and IgM anti-T. gondii, respectively.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence and risk factors of Toxoplasma gondii infection among Cancer and Hemodialysis Patients in southwest Iran

Arefkhah

Hosseini

Karimzade

et al. 2019

Clinical Epidemiology and Global Health

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Background: Due to their poor immune system, cancer patients undergoing chemotherapy and hemodialysis patients are more at risk for toxoplasmosis and its complications than the healthy people. The current study aimed to determine prevalence of toxoplasmosis in these patients and comparing it with healthy subjects in southwest Iran. Methods: This cross-sectional study was performed on sera and buffy coat of 100 cancer patients, 47 hemodialysis patients, and 170 healthy subjects. IgG and IgM anti-Toxoplasma gondii antibodies in serum were measured by ELISA method. Molecular diagnosis was conducted by PCR method on buffy coat of the seropositive samples. Results: The seroprevalence of T. gondii in cancer, hemodialysis patients and healthy subjects were 13%, 27.7% and 15.9% respectively. Moreover, seropositivity for IgM antibody was 2.1% in hemodialysis, 2% in cancer patients and 0.6% in healthy individuals. Our results were showed there was no significant difference between prevalence toxoplasmosis in case and control group. In molecular survey, only one case (cancer patient) was positive for Toxoplasma DNA. Contact with cats and consumption of undercooked meat were two studied risk factors which had significant associations with T. gondii seropositivity in the hemodialysis patients (odds ratio [OR] = 14.667; 95% confidence interval [CI] = 1.453-148.045) and control (odds ratio [OR] = 3.07; 95% confidence interval [CI] = 1.093-8.639) respectively. Conclusion: Seroprevalence of toxoplasmosis in hemodialysis patients was higher than Healthy Individuals; however, the seroprevalence of toxoplasmosis in cancer patients was similar to the Healthy Individuals.

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“…This result is consistent with the rate reported by Yazar et al [ 53 ] in Turkey (1.7%), but lower than that reported by Aufy et al [ 54 ] in Egypt (16.7%). Moreover, among the included studies, only 3 [ 42 , 47 , 48 ] applied molecular methods (polymerase chain reaction) to detect T. gondii infection. In all those studies, active infection (circulating DNA) was only observed in hemodialysis patients, whereas no controls showed positive results [ 42 , 47 , 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, among the included studies, only 3 [ 42 , 47 , 48 ] applied molecular methods (polymerase chain reaction) to detect T. gondii infection. In all those studies, active infection (circulating DNA) was only observed in hemodialysis patients, whereas no controls showed positive results [ 42 , 47 , 48 ]. This result indicates that hemodialysis patients were more susceptible to acquire T. gondii infection.…”

Section: Discussionmentioning

confidence: 99%

A systematic review and meta-analysis of the prevalence of toxoplasmosis in hemodialysis patients in Iran

Foroutan

Rostami²,

Majidiani

et al. 2018

Epidemiol Health

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OBJECTIVESToxoplasmosis is a parasitic disease that occurs worldwide, with a wide range of complications in immunocompromised patients. This systematic review and meta-analysis was performed to evaluate the seroprevalence of Toxoplasma gondii among patients undergoing hemodialysis in Iran.METHODSWe searched English and Persian databases for studies reporting T. gondii seroprevalence in Iranian hemodialysis patients through December 31, 2017. Inclusion and exclusion criteria were applied.RESULTSA total of 10 studies containing 1,865 participants (1,048 patients and 817 controls) met the eligibility criteria. Immunoglobulin G (IgG) antibodies against T. gondii were found in 58% (95% confidence interval [CI], 46 to 70) of hemodialysis patients and 40% (95% CI, 31 to 50) of healthy controls, while immunoglobulin M (IgM) antibodies were found in 2% (95% CI, 0 to 6) of hemodialysis patients and 0% (95% CI, 0 to 1) of healthy controls. The meta-analysis showed that hemodialysis patients were significantly more likely to be seropositive for IgG (odds ratio [OR], 2.04; 95% CI, 1.54 to 2.70; p<0.001) and IgM (OR, 2.53; 95% CI, 1.23 to 5.22; p<0.001) antibodies against T. gondii infection than healthy individuals.CONCLUSIONSThe current study revealed a high prevalence of T. gondii infection in hemodialysis patients. Since hemodialysis patients are immunocompromised and T. gondii can cause serious clinical complications, we recommend that periodic screenings for T. gondii infection should be incorporated into the routine clinical care of these patients.

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Identification and determination of the prevalence of Toxoplasma gondii in patients with chronic renal failure by ELISA and PCR

Cited by 10 publications

References 15 publications

Evaluation of Anti-Toxoplasma gondii Antibodies in Hemodialysis Patients with Chronic Kidney Disease in Sari, Iran

Evaluation of Anti-Toxoplasma gondii Antibodies in Hemodialysis Patients with Chronic Kidney Disease in Sari, Iran

Seroprevalence and risk factors of Toxoplasma gondii infection among Cancer and Hemodialysis Patients in southwest Iran

A systematic review and meta-analysis of the prevalence of toxoplasmosis in hemodialysis patients in Iran

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