Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle

Bihannic, Morgan; Ghanbarpour, Reza; Auvray, Frédéric; Cavalié, Laurent; Châtre, Pierre; Boury, Michèle; Brugère, Hubert; Madec, Jean‐Yves; Oswald, Éric

doi:10.1186/s13567-014-0076-9

Cited by 18 publications

(21 citation statements)

References 38 publications

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“…These results were almost similar to other studies which demonstrated that the isolates of phylogroup A were prevalent in the intestinal tracts of most animals 24,37 and phylogroup B1 was dominant among isolates from healthy cattle. 38 In accordance with the previous reports in Iran, 23,39 this study showed that the phylogroup B1 was dominant in calves. The phylogroup B2 had also the least frequency, both in calves and dairy cows, and these outcomes confirmed the previous investigations.…”

Section: Discussionsupporting

confidence: 81%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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Background: Food-producing animals are under suspicion for the reservoir and colonization of ESBL (extended-spectrum beta-lactamase)-producing bacteria especially Enterobacteriaceae and therefore infection of the humans with them. The increasing reports on the ESBLs presence in the pathogenic and commensal Escherichia coli isolates have been a concern worldwide. These strains can be attributed to one of the main phylogenetic groups and subgroups. Several studies have shown the relationship between the phylogeny and antimicrobial resistance of E. coli strains. Objectives: The aim of this study was to analyze the phylogenetic group of ESBL-producing E. coli and detect its phenotype using the multiplex polymerase chain reaction (PCR) and combined disk method. Materials and Methods: Two hundred five E. coli fecal isolates were obtained from 103 calves (90 healthy and 13 diarrheic) and 102 dairy cows (healthy) from 8 farms in Khuzestan province, Iran. The triplex PCR method was used to allocate the E. coli isolates based on the presence or absence of 3 genes (chuA, yjaA, and tspE4.C2) to yield 4 definite phylogenetic groups and 7 subgroups. Phenotypic ESBL-producing E. coli was determined using the double disk diffusion method according to the manufacturer's instructions and Clinical & Laboratory Standards Institute (CLSI) guidelines. Results: A total of 65.04% and 22.3% of isolates from calves and 70.5% and 20.5% of isolates from dairy cows belonged to phylogroups B1 and A, respectively. In addition, no isolate from the diarrhoeic calves was found to belong to group B2 and subgroups D2 and A0. A low prevalence (2/205 isolates, 0.97%) of ESBL-producing E. coli was found only in the samples of dairy cows which belonged to the phylogenetic group A and phylogenetic subgroup A1. There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between the E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Conclusion: There was no statistically significant relationship between the phylogenetic group and the production of ESBLs (P = 0.11). There was also no difference between E. coli isolates from calves and dairy cows in the production of ESBLs (P = 0.5). Based on these results, there is a low prevalence of ESBL-producing E. coli in the dairy farms of Khuzestan province. However, further large scale investigations are necessary to control the antibiotic resistance in the human and animal foodstuff.

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Section: Discussionsupporting

confidence: 81%

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Barzan¹,

Gharibi²,

Ghorbanpoor³

et al. 2017

Int J Enteric Pathog

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“…Other studies revealed that phylogroup B1 was dominant among isolates from healthy cattle (Alizade et al, 2014). The analysis demonstrated that phylogroup B1 was more prevalent in isolates from diarrhoeic and septicemic calves in Iran (Ghanbarpour et al, 2009;Bihannic et al, 2014;Staji et al, 2015). It is interesting to note that subgroup B2 3 was found among isolates from both healthy and diarrhoeic calves, whereas subgroup A 0 was not found in all isolates.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic analysis of Escherichia coli isolates from healthy and diarrhoeic calves in Mashhad, Iran.

Barzan¹,

Rad²,

Tabar³

et al. 2017

BJVM

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Escherichia coli is a normal inhabitant of the gastrointestinal tract of vertebrates. Certain Escherichia coli strains have been associated with neonatal diarrhoea in ruminants. These strains can be assigned to one of the four main phylogenetic groups, A, B1, B2 and D. Several studies have shown the relationship between phylogeny and pathogenicity of E. coli, a great deal can be obtained by determining the phylogroup of unknown E. coli strains. In this study, we aimed to evaluate the influence of diarrhoea on the genetic composition of E. coli populations isolated from calves. A total of 80 Escherichia coli isolates were obtained from healthy and diarrhoeic calves. Phylogenetic grouping was done based on the Clermont triplex PCR method using primers targeted at three genetic markers, chuA, yjaA and TspE4.C2. According to our results, phylogenetic group A strains was the most prevalent in both healthy (37.5%) and diarrhoeic calves (55%). Group B1 contained 27.5% of isolates in healthy calves, followed by group B2 (17.5%), and group D (7.5%). Also, four isolates from healthy calves were not included in the major phylogenetic groups or subgroups. A total of 14% and 4% of isolates from diarrhoeic calves beloned to phylogroups B2 and D respectively. Although no isolate from diarrhoeic calves was found to belong to group B1, there was no significant difference between healthy and diarrhoeic calves for other phylogroups. There was not a dramatic shift in E. coli phylogroup/subgroup due to occurrence of diarrhoea in calves, except for phylogroup B1 which was higher in healthy calves. This can be due to the difference in secretions of digestive system in diarrhoeic calves which can prevent the conditions for instability of Escherichia coli isolates of phylogroup B1. The majority of isolates from both healthy and diarrhoeic calves belonged to nonpathogenic phylogentic group A and B1.

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“…All the fimbriae in Table 1 have been found associated with enterotoxin (genes) on clinical isolates of animals with diarrhea. However, F17 fimbriae are also expressed by diarrheagenic non-ETEC strains or by extra-intestinal E. coli (82), (83–85). Clinical isolates of ETEC have clonal properties, since there is a preferential association of fimbriae with certain enterotoxins and O-serotypes (Table 1) that have lead to their classification into major pathotypes (86).…”

Section: The Role Of Fimbriae In Etec Pathogenesismentioning

confidence: 99%

“…The crystal structures of the immunoglobulin-like lectin domains of two F17 adhesins (F17G and the related adhesin GafD) that bind N-acetylglucosamine were resolved (217, 218). Although an f17G gene encoding a different N-terminal sequence was recently described, it was not carried by an ETEC strain (85). …”

Section: Adhesins and Host Receptorsmentioning

confidence: 99%

Animal EnterotoxigenicEscherichia coli

2016

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Enterotoxigenic Escherichia coli (ETEC) is the most common cause of E. coli diarrhea in farm animals. ETEC are characterized by the ability to produce two types of virulence factors; adhesins that promote binding to specific enterocyte receptors for intestinal colonization and enterotoxins responsible for fluid secretion. The best-characterized adhesins are expressed in the context of fimbriae, such as the F4 (also designated K88), F5 (K99), F6 (987P), F17 and F18 fimbriae. Once established in the animal small intestine, ETEC produces enterotoxin(s) that lead to diarrhea. The enterotoxins belong to two major classes; heat-labile toxin that consist of one active and five binding subunits (LT), and heat-stable toxins that are small polypeptides (STa, STb, and EAST1). This chapter describes the disease and pathogenesis of animal ETEC, the corresponding virulence genes and protein products of these bacteria, their regulation and targets in animal hosts, as well as mechanisms of action. Furthermore, vaccines, inhibitors, probiotics and the identification of potential new targets identified by genomics are presented in the context of animal ETEC.

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Identification and detection of three new F17 fimbrial variants in Escherichia coli strains isolated from cattle

Cited by 18 publications

References 38 publications

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Phylogenetic Grouping and Phenotypic Detection of Extended-Spectrum β-Lactamases Among Escherichia coli From Calves and Dairy Cows in Khuzestan, Iran

Phylogenetic analysis of Escherichia coli isolates from healthy and diarrhoeic calves in Mashhad, Iran.

Animal EnterotoxigenicEscherichia coli

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