Identification and conformational analysis of putative microRNAs in Maruca vitrata (Lepidoptera: Pyralidae)

Abstract

MicroRNAs (miRNAs) are a class of small RNAs, evolutionarily conserved endogenous non-coding RNAs that regulate their target mRNA expression by either inactivating or degrading mRNA genes; thus playing an important role in the growth and development of an organism. Maruca vitrata is an insect pest of leguminous plants like pigeon pea, cowpea and mung bean and is pantropical. In this study, we perform BLAST on all known miRNAs against the transcriptome data of M. vitrata and thirteen miRNAs were identified. These miRNAs were characterised and their target genes were identified using TargetScan and were functionally annotated using FlyBase. The importance of the structure of pre-miRNA in the Drosha activity led to study the backbone torsion angles of predicted pre-miRNAs (mvi-miR-9751, mvi-miR-649-3p, mvi-miR-4057 and mvi-miR-1271) to identify various nucleotide triplets that contribute to the variation of torsion angle values at various structural motifs of a pre-miRNA.

Identification and Characterization of Small Noncoding RNAs in Genome Sequences of the Edible FungusPleurotus ostreatus

“…These conserved sncRNAs included the snRNA U2 (4 candidates) and U6 (3 candidates), 10 miRNAs (miR2673a, miR2673b, and miR-4968-3p), and 57 tRNAs (see Supplement Table 4). In some previous researches, the microRNAs miR2673 and miR-4968-3p were found to have many target genes in many species [ 66 , 67 ] and may regulate some targets [ 68 , 69 ]. MicroRNA miR2673 was also be found to have stable structure and be conserved across plant species [ 70 ].…”

Application of insulin signaling to predict insect growth rate in Maruca vitrata (Lepidoptera: Crambidae)

“…It exhibits host-associated variation in development [ 21 ]. Its developmental difference is partially explained by post-transcriptional action of 13 micro RNAs against genes associated with cell signaling, metabolism, and metamorphosis [ 22 ]. Especially, its nutrient-based growth can be understood by IIS and its downstream physiological roles because nutrients are essential for all cell growth.…”

Molecular Mechanisms of miR-1271 Dysregulation in Human Cancer

“…M icroRNA is a type of small noncoding RNA that inactivates or decreases the expression of target genes by regulating the target mRNA (Shruthi Sureshan and Habeeb, 2015). It is a large family of highly conserved noncoding genes that negatively regulate the expression of a wide variety of genes by binding to complementary sequences in the 3¢-untranslated regions (UTRs) of target mRNAs (Liu et al, 2019a).…”