Identification and Confirmation of SSR Marker Tightly Linked to the Ti Locus in Soybean [Glycine max (L.) Merr.]

Chung, Jong Il

doi:10.5772/18771

Cited by 2 publications

(3 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…SSR markers are well known as being polymorphic, with codominant inheritance, and therefore can be widely used for genotype identification and selection of desired traits [23]. However, the distance between the SSR marker and the gene of interest can vary depending on the type of population [6,24]. For example, the genetic distance between Satt228 and Ti3 varied between 0 and 3.7 cM in two different populations [23].…”

Section: Discussionmentioning

confidence: 99%

“…For soybean seeds with high KTI, a preliminary heat treatment is required to inactivate the trypsin inhibitor enzyme before it can be fed to poultry and livestock. This heat treatment affectively destroys the undesirable anti-nutritional component, but also leads to an increase in the cost of the final product as well as decreased levels of available amino acids [6].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

et al. 2019

View full text Add to dashboard Cite

A soybean germplasm collection was studied for the identification of accessions with low trypsin inhibitor content in seeds. Twenty-nine accessions, parental plants, and two hybrid populations were selected and analyzed using genetic markers for alleles of the Ti3 locus, encoding Kunitz trypsin inhibitor (KTI). Most of the accessions had high or very high KTI (49.22–73.53 Trypsin units inhibited (TUI/mg seeds), while the two local Kazakh cultivars, Lastochka and Ivushka, were found to have a moderately high content of KTI, at 54.16–54.87 TUI/mg. In contrast, two soybean cultivars from Italy, Hilario and Ascasubi, showed the lowest levels of trypsin units inhibited, at 25.47–27.87 TUI/mg. Electrophoresis of seed proteins in a non-denaturing system showed a simple discrimination pattern and very clear presence/absence of bands corresponding to KTI. The SSR marker Satt228 was the most effective diagnostic marker among the three examined, and it confirmed the presence of the homozygous null-allele ti3/ti3 in cultivars Ascasubi and Hilario, which were used for hybridization with the local cv. Lastochka. Heterozygote F1 hybrid plants and homozygous ti3/ti3 lines in F2 segregating populations were successfully identified using Satt228. Finally, through marker-assisted selection with Satt228, prospective homozygous ti3/ti3 lines were produced for further application in the breeding program aimed at improving soybean seed quality in Kazakhstan.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For soybean seeds with high KTI, a preliminary heat, before use of the seeds is recommended, heat treatment effectively destroys is required to inactivate the trypsin inhibitor enzyme. This procedure has the disadvantage of decreased levels of available amino acids with additional increasing final product cost (Liencer, 1995;Palacios et al, 2004;Chung, 2011;Kumar et al, 2013;2015).…”

Section: Introductionmentioning

confidence: 99%

Research Article Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (<i>Glycine</i> <i>max</i>) by simple sequence repeat marker assisted selection

da Silva,

Carpentieri-Pipolo,

Barreto

et al. 2023

Genet. Mol. Res.

View full text Add to dashboard Cite

the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx 2 null-allele in the parental cultivar BRS 213 by flanking Lx 2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality.

show abstract

Identification and Confirmation of SSR Marker Tightly Linked to the Ti Locus in Soybean [Glycine max (L.) Merr.]

Cited by 2 publications

References 18 publications

Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

Trypsin Inhibitor Assessment with Biochemical and Molecular Markers in a Soybean Germplasm Collection and Hybrid Populations for Seed Quality Improvement

Research Article Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (<i>Glycine</i> <i>max</i>) by simple sequence repeat marker assisted selection

Contact Info

Product

Resources

About