Research Article Genetic removal of trypsin inhibitor and lipoxygenase isozymes form soybean seeds (<i>Glycine</i> <i>max</i>) by simple sequence repeat marker assisted selection

Abstract

the KTi/kti-gene-specific marker does not allow for the identification of heterozygous and homozygous genotypes with dominant KTi alleles; the the simple sequence repeat (SSR) markers Satt228 and Satt409, which bind tightly to the KTi locus, were much more suitable diagnostic marker for screening plants in segregating populations. Satt090 and Satt417 confirmed the presence of the homozygous Lx 2 null-allele in the parental cultivar BRS 213 by flanking Lx 2 loci at 3.00 and 2.77 cM, respectively. The SSR markers used in this study could be efficiently used in marker assisted selection in a breeding program aimed at improving soybean seed quality.