Identification and confirmation of chemical residues in food by chromatography-mass spectrometry and other techniques

Lehotay, Steven J.; Maštovská, Kateřina; Amirav, Aviv; Fialkov, Alexander B.; Martos, Perry; Kok, Albert; Fernández‐Alba, Amadeo R.

doi:10.1016/j.trac.2008.10.004

Cited by 118 publications

(105 citation statements)

References 45 publications

Supporting

Mentioning

103

Contrasting

Unclassified

Order By: Relevance

“…This figure highlights how peak shapes (criterion #2 listed above) are very helpful in practice for identification purposes, and it is unfortunate that this aspect involving common sense is considered to be too nuanced in other identification schemes. [27] Figures 4 and 5 demonstrate that a consistent low-level interferant occurs for ions 2 and 3, but not ion 1 (the quantification ion). Indeed, response linearity and quantification results based on ion 1 for cimaterol were very good, even though the identification results based on ions 2 and 3 were poor.…”

Section: Uhplc-ms/ms Identificationmentioning

confidence: 87%

“…In this article, we use the terminology of screening, identification, confirmation, and determination described in Lehotay et al [27] In brief, 'screening' involves simple yes/no decisions from moderately selective tests, such as the bioassay or the UHPLC-MS screen using t R and signal from one ion transition (as described in the next section); 'identification' entails yes/no decisions from highly selective methods, such as the UHPLC-MS/MS approach using 3 ion transitions (as described in the section to follow); 'confirmation' involves at least two analyses in agreement, one or both of which meet identification criteria and preferably entail different aspects of chemistry for analysis (orthogonal selectivity); and 'determination' is quantification using an appropriately validated method (the official regulatory method in this case).…”

Section: Qualitative Analysismentioning

confidence: 99%

“…Therefore, we employed relatively liberal identification criteria to reduce false negatives and rely on confirmation to eliminate the chance of false positives. [27] The final identification criteria for our purposes were defined as follows:…”

Section: Uhplc-ms/ms Identificationmentioning

confidence: 99%

See 2 more Smart Citations

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Lehotay

Lightfield

Geis-Asteggiante³

et al. 2012

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

In the USA, the US Department of Agriculture's Food Safety and Inspection Service (FSIS) conducts the National Residue Program designed to monitor veterinary drug and other chemical residues in beef and other slaughtered food animals. Currently, FSIS uses a 7-plate bioassay in the laboratory to screen for antimicrobial drugs in bovine kidneys from those animals tested positive by inspectors in the slaughter establishments. The microbial inhibition bioassay has several limitations in terms of monitoring scope, sensitivity, selectivity, and analysis time. Ultra-high performance liquid chromatography -tandem mass spectrometry (UHPLC-MS/MS) has many advantages over the bioassay for this application, and this study was designed to develop, evaluate, and validate a fast UHPLC-MS/MS method for antibiotics and other high-priority veterinary drugs in bovine kidney. Five existing multi-class, multi-residue methods from the literature were tested and compared, and each performed similarly. Experiments with incurred samples demonstrated that a 5-min shake of 2 g homogenized kidney with 10 ml of 4/1 (v/v) acetonitrile/water followed by simultaneous clean-up of the initial extract with 0.5 g C18 and 10 ml hexane gave a fast, simple, and effective sample preparation method for the <10 min UHPLC-MS/MS analysis. An extensive 5-day validation process demonstrated that the final method could be used to acceptably screen for 54 of the 62 drugs tested, and 50 of those met qualitative MS identification criteria. Quantification was not needed in the application, but the method gave ≥70% recoveries and ≤25% reproducibilities for 30 of the drugs. Published 2012. This article is a U.S. Government work and is in the public domain of the USA.

show abstract

Section: Uhplc-ms/ms Identificationmentioning

confidence: 87%

Section: Qualitative Analysismentioning

confidence: 99%

See 1 more Smart Citation

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Lehotay

Lightfield

Geis-Asteggiante³

et al. 2012

Drug Testing and Analysis

Self Cite

View full text Add to dashboard Cite

show abstract

“…This is not a novel concept and has already been suggested elsewhere. [1] If available, alternative transitions should be evaluated.…”

Section: Implications For the Confirmation Processmentioning

confidence: 99%

“…As a result, a single analysis, no matter how selective it may be, is not confirmatory". [1] The CD 2002/657/EC specifies that the term "Confirmatory method" refers to methods that provide full or complementary information enabling the substance to be unequivocally identified at the level of interest. [2] Currently, identification and confirmation are most often achieved by techniques that provide structural information (e.g.…”

mentioning

confidence: 99%

Signal suppression can bias selected reaction monitoring ratios. Implications for the confirmation of positive findings in residue testing

Kaufmann¹,

Widmer²,

Maden³

2014

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

The [M+H](+) ion may be the most commonly used precursor ion in liquid chromatography coupled to electrospray operated in the positive ionization mode. However, the absence of a sufficiently intensive confirmation transition frequently leads to the selection of another precursor ion to be utilized for the confirmation transition. The SRM ratio derived from such transitions should not be compared to the SRM ratio derived from pure standard solutions but spiked blank matrix extracts.

show abstract

Pesticides (New Generation) and Related Compounds, Analysis of

Picó

2009

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Miscellaneous pesticides and related compounds comprise substances that can neither be included in the classical chemical category of pesticides nor classified by their mode of action. They are a heterogeneous group of substances, the most representative of which are macrocyclic lactones, chloronicotinyls, tetranortriterpenoids, ammonium quaternary salts, dinitroanilines, acetamides, oximes, triazoles, and pyridine‐based molecules. The pesticides covered in this article have hardly changed in the last years. However, their analytical determination has undergone significant improvements. This article provides a well‐defined and critical compilation of the sample treatment and clean‐up procedures, as well as determination techniques applied to miscellaneous pesticides. The new approaches in sample preparation in environmental matrices, such as air, water, soil, food and biological samples, are described. Analytical techniques for characterizing these compounds, which mostly include gas chromatography (GC) and high‐performance liquid chromatography (HPLC) coupled to mass spectrometry (MS), are discussed. Both techniques have almost completely displaced other chromatographic methods. However, in the frontier between chemical and biological techniques, the development of biosensor techniques is very promising. Here, analytical methods applied to the field of real samples are also presented.

show abstract

Identification and confirmation of chemical residues in food by chromatography-mass spectrometry and other techniques

Cited by 118 publications

References 45 publications

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Development and validation of a streamlined method designed to detect residues of 62 veterinary drugs in bovine kidney using ultra‐high performance liquid chromatography – tandem mass spectrometry

Signal suppression can bias selected reaction monitoring ratios. Implications for the confirmation of positive findings in residue testing

Pesticides (New Generation) and Related Compounds, Analysis of

Contact Info

Product

Resources

About