IDENTIFICATION AND CHROMOMERIC INTERPRETATION OF PACHYTENE BIVALENTS FROM CANIS FAMILIARIS

Abstract

Squash techniques were used to spread, the 39 pachytene bivalents from spermatogenic Canis familiaris cells collected by testicular biopsy from normal, healthy male collie dogs. Eight cells in which all bivalents could be identified were photographed, enlarged to 4500 × and cutouts prepared for each chromosome in each of the cells. Characterization and identification were based on relative length, mean percentage length, and number of chromomeres in each bivalent.

Heterochromatin, synaptonemal complex, and NOR activity in the somatic and germ cells of a male domestic dog, <i>Canis familiaris</i>(Mammalia, Canidae)

“…Such techniques, however, have not been applied to the meiotic chromosomes of this species. Only conventionally stained meiotic preparations of dog have been reported (E uasson et al, 1968; Ford, 1969Ford, : W illiams et al, 1971.…”