Identification and characterization ofPodophyllum emodi by API-LC/MS/MS

Abstract

An API-LC/MS/MS method was developed for the identification of the medicinal herb Podophyllum emodi based on the profile of its aryltetrahydronapthalene and related lignan marker compounds. This was done by matching the structural information from the tandem mass spectrometric data with those lignan marker compounds already reported for the herb. The method could be employed in the absence of reference standards for the markers and was particularly useful in view of the scarcity of supply of these chemical standards. It has been used successfully to differentiate Podophyllum emodi from two commonly used medicinal herbs of a different genus but having similar appearance, Radix clematidis and Radix gentiana, as well as a closely related herb, Podophyllum peltatum.

Chemical Fingerprinting of Medicinal Plants “Gui-jiu” by LC-ESI Multiple-Stage MS

“…However, LC-MS provides a more accurate means of authentication, as the individual components in the fingerprint can be elucidated. Previously, an LC-API-MS method was reported for the identification and characterization of Podophyllum emodi [29]. Our report provides the first establishment of LC-UV-ESI-MS fingerprints for D. versipelli, D. pleiantha, and S. emodi, which are listed as ''Gui-jiu''.…”

Arylnaphthalene and aryltetralin‐type lignans in hairy root cultures of Linum perenne, and the stereochemistry of 6‐methoxypodophyllotoxin and one diastereoisomer by HPLC‐MS and NMR spectroscopy

“…Among them, ions at m/z 343, m/z 259, m/z 215 and m/z 312 were characteristic ions in both spectrR. The fragment ion at m/z 343.1 was formed by the loss of the C‐7 oxygen and elimination of the lactone moiety followed by a retro‐Diels–Alders (RDA) rearrangement (loss of the crotonolactone molecule, C 4 H 4 O 2 , 84 Da) leading to a stabilised anthracene derivative [M + H − H 2 O − C 4 H 4 O2] + (Wong et al ., ). The fragment ion at m/z 312.1 is the result of the loss of methoxy (OCH 3 ) from the fragment ion at m/z 343.1.…”

Characterization of in vitro metabolites of deoxypodophyllotoxin in human and rat liver microsomes using liquid chromatography/tandem mass spectrometry

“…MS 2 of protonated DPT yielded a major product ion at m/z 231 and MS 3 of the ion at m/z 231 generated product ions at m/z 213 and 187. The product ion at m/z 231 indicated the loss of a trimethoxybenzene moiety and subsequent loss of H 2 O (18 Da) and CO 2 (44 Da) in MS 3 resulted in the generation of the ions at m/z 213 and 187, respectively 14–16…”