Chemical Fingerprinting of Medicinal Plants “Gui-jiu” by LC-ESI Multiple-Stage MS

Abstract

The rhizomes or roots of Dysosma versipellis (Hance) M. Cheng, Dysosma pleiantha (Hance) Woodson, and Sinopodophyllum emodi (Wall. Ex Royle) Ying under the same name ''Guijiu'', were widely used for medicinal purposes. Valid quality control of Gui-jiu is desirable due to the fact that they are highly toxic. In the present paper, an accurate fingerprint method was developed to identify the three species. Liquid chromatography coupled with photodiode array detection and electrospray ionization-multiple stage mass spectrometry were developed to identify the fingerprint components. According to the characteristic fragmentation behavior of known lignans and flavonoids isolated from D. versipellis, a total of 15 constituents in the crude extracts were structurally characterized on the basis of retention time, UV and tandem mass spectrometric analysis. Eight batches of D. versipellis, three batches of D. pleiantha, and two batches of S. emodi were collected from different locations and these representative samples reflected the similar chemical constituent properties. The extracts were separated by a C18 reversed phase LC column, with a gradient solvent system. The proposed method provides a scientific and technical platform to the herbal industry for quality control and safety assurance of herbal preparations that contain this class of poisonous podophyllotoxin-type lignans.

Botanicals inRibes nigrumbud-preparations: An analytical fingerprinting to evaluate the bioactive contribution to total phytocomplex

“…In the macerated samples, most constituents were also weakly acid, so adding formic acid was necessary for enhancing the resolution and eliminating peak tailing . The choice of detection wavelength was a crucial step for developing a reliable fingerprint Zhou et al, 2008). A full-scan on the chromatogram from 190 to 400 nm was performed and only selected wavelengths were suitable to achieve more specific peaks as well as a smooth baseline.…”

“…However, it should be noted that many traditional preparations are composed of multiple herbs, so that the analysis of selected constituents might not reflect their overall quality or efficacy (Qiao et al, 2010;Steinmann & Ganzera, 2011;Zhao et al, 2009;Zhou et al, 2008). Different kinds of features can be selected to characterize the herbal preparations, and referred to as the overall fingerprint: genetic, quality, sensory or morphological features could be used to create a fingerprint as showed in other studies (Canterino et al, 2012;Mellano et al, 2012).…”

Therapeutic values, chemical constituents and toxicity of Taiwanese Dysosma pleiantha – A review

“…The roots and rhizome of Bajiaolian have been investigated for its chemical constituents by thin layer chromatography (TLC), high performance liquid chromatography (HPLC) and liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) (Jackson and Dewick 1985;Zhou et al, 2008). The main constituents of roots and rhizome of Bajiaolian are lignans and flavonoids.…”

Recent developments in qualitative and quantitative analysis of phytochemical constituents and their metabolites using liquid chromatography–mass spectrometry

“…Zhou et al developed a chemical fingerprinting method to identify the Dysosma versipellis, Dysosma pleiantha and Sinopodophyllum emodi which are listed as "Gui-jiu" [218]. "Gui-jiu", a toxic herbal material in Chinese medicine, is widely used for the treatment of rheumatoid arthritis with numbness of the limbs, and pyogenic infection of skin tissue.…”