Identification and characterization of the pathogenic effect of a Vibrio parahaemolyticus-related bacterium isolated from clam Meretrix meretrix with mass mortality

Yue, Xin; Liu, Baozhong; Xiang, Jianhai; Jia, Jingdun

doi:10.1016/j.jip.2009.11.008

Cited by 69 publications

(50 citation statements)

References 32 publications

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“…Among those, species from the genus Vibrio are an important cause of diseases in cultured shellfish [20,26]. For example, V. anguillarum is found to be one of the major pathogens causing vibriosis in China [19].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Zhang

Liu

Chen

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

“…In the past decades, clams have been reported to experience massive mortality in China, resulted in serious economic losses [19,20]. There are several suspected causes of such massive mortality, such as the prevalence of Vibrio and virus pathogenic microorganisms, deterioration of water quality caused by chemical pollution [19].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Zhang

Liu

Chen

et al. 2011

Fish & Shellfish Immunology

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“…It is also a highpriced commercial commodity in the fishery trade. After generations of successful aquaculture, the clam industry in China has experienced serious losses from disease-induced mortality (Yue et al 2010). It is suspected that deteriorating environmental quality is one of the primary causes of disease in aquaculture.…”

Section: Abstract: Hard Clam · Meretrix Meretrix · Hsp20 · Mrna Exprmentioning

confidence: 99%

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

Zhang¹,

Liu²,

He³

et al. 2013

Aquat. Biol.

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Heat shock proteins (HSPs) are molecular chaperones that help organisms cope with stressful conditions. In this study, a novel small HSP (sHSP) gene was identified from the hard clam Meretrix meretrix (designated as Mm-HSP20). The full length of the cDNA is 1222 bp, consisting of a 5'-terminal untranslated region (5'UTR) of 103 bp, a 3'UTR of 565 bp, and a 522 bp open reading frame encoding a polypeptide of 173 amino acids. Sequence comparison showed that Mm-HSP20 had a moderate degree of homology to the sHSP of other organisms. A sHSP feature domain, an alpha-crystallin domain, and a V/IXI/V motif in the C-terminal extension were identified in the Mm-HSP20 amino acid sequence, indicating that Mm-HSP20 is a new member of the sHSP family. The Mm-HSP20 transcript was constitutively expressed in 6 different test tissues, with the highest expression level detected in the digestive gland by fluorescent quantitative realtime PCR. The temporal expression of Mm-HSP20 mRNA in the digestive gland and hemocytes was evaluated after exposure to cadmium (Cd) (40 µg l −1 CdCl 2 ) and benzo [a]pyrene (50 µg l −1 B[a]P) individually, and in combination (40 µg l −1 CdCl 2 + 5 0 µg l −1 B[a]P). Mm-HSP20 expression level increased significantly after Cd and B[a]P exposure in both tissue types. Conversely, significant Mm-HSP20 transcript repression was detected in the 24 h B[a]P-treated digestive gland samples. Considerable up-regulation of Mm-HSP20 mRNA level was observed after multiple exposures. These results indicate that Mm-HSP20 plays a role in mechanisms involved in coping with environmental stress in hard clams. Mm-HSP20 may be suitable for use as a biomarker for heavy metal and B[a]P contamination. KEY WORDS: Hard clam · Meretrix meretrix · HSP20 · mRNA expression · Heavy metal · Cd · Benzo[a]pyrene · BiomarkerResale or republication not permitted without written consent of the publisher Aquat Biol 18: 243-252, 2013 sources, they bioaccumulate, and they are not biodegradable (Zhang et al. 2006). Among these, cadmium (Cd) is one of the most abundant and ubiquitously distributed toxic elements found in aquatic systems (Yuan et al. 2004, Wan et al. 2008). The concentrations of heavy metals observed in coastal sediments are several times higher than those in water columns, and they can have considerable adverse effects on marine organisms. The bioaccumulation of sediment-bound metals in benthic species is extremely important in food webs and ultimately to humans (Wang 2002).Benzo [a]pyrene (B[a]P) is a representative polycyclic aromatic hydrocarbon (PAH). It is therefore classified as a pervasive environmental contaminant. B[a]P is found in water columns and sediments (Men et al. 2009). It enters marine environments via anthropogenic sources, including petroleum spillage, industrial discharge, and urban runoff (Soclo et al. 2000, Boonyatumanond et al. 2006. B[a]P bioaccumulation causes damage to various organ systems in exposed aquatic organisms. Toxicological studies have demonstrated the effects of B[a]P on organ...

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“…For example, Vibrio and other pathogenic bacteria were frequently found in M. galloprovincialis [17,18]. Among those pathogens, Vibrio species have been demonstrated to be an important cause of diseases in shellfish [19], which could induce cytotoxicity and the production of reactive oxygen (ROS), etc. [20].…”

Section: Introductionmentioning

confidence: 99%

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

You

Ning²,

Liu³

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tHeat shock 70 kDa protein 12A (HSPA12A) is an atypical member of HSP70 family, and the translationally controlled tumor protein (TCTP) is a novel HSP with chaperone-like activity. They are both involved in protecting organisms against various stressors. In the present study, the cDNAs of HSPA12A and TCTP (called MgHSPA12A and MgTCTP) were identified from Mytilus galloprovincialis by RACE approaches. The full-length cDNA of MgHSPA12A and MgTCTP encoded a peptide of 491 and 171 amino acids, respectively. Real-time PCR was employed to analyze the tissue distribution and temporal expression of these two genes after bacterial challenge and cadmium (Cd) exposure. It was found that the transcripts of MgHSPA12A and MgTCTP were dominantly expressed in gonad and muscle, respectively. The expression level of MgTCTP at 48 h post Vibrio anguillarum challenge was detected to be significantly up-regulated in hepatopancreas (P < 0.05). As concerned to Cd exposure, 2.0-fold increase of MgHSPA12A expression compared to that of the control was observed at 48 h in 5 mg/L Cd 2þ -treated group, while the expression levels of MgTCTP were significantly decreased after exposed to both 5 and 50 mg/L Cd 2þ for 24 h and 96 h.These results suggested the potential involvement of MgHSPA12A and MgTCTP in the mediation of the immune responses and environmental stress in mussels.

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Identification and characterization of the pathogenic effect of a Vibrio parahaemolyticus-related bacterium isolated from clam Meretrix meretrix with mass mortality

Cited by 69 publications

References 32 publications

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Transcriptional regulation of selenium-dependent glutathione peroxidase from Venerupis philippinarum in response to pathogen and contaminants challenge

Identification of a small HSP gene from hard clam Meretrix meretrix and its potential as an environmental stress biomarker

The response profiles of HSPA12A and TCTP from Mytilus galloprovincialis to pathogen and cadmium challenge

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