Identification and characterization of peanut stunt cucumovirus from naturally infected alfalfa in Iran

Abstract

A mechanically transmissible virus was isolated from a naturally infected alfalfa plant (Medicago sativa) in Karaj, Iran. It induced fern-leaf symptoms in Lycopersicon esculentum, general chlorosis and stunting in Arachis hypogoea, local lesions and systemic infection in Chenopodium quinoa, Phaseolus vulgaris and Vigna unguiculata but only local lesions in C. amaranticolor. In gel-immunodiffusion tests, it reacted strongly with an antiserum to peanut stunt cucumovirus (PSV), moderately with two of six antisera to cucumber mosaic cucumovirus (CMV) and with two of five antisera to tomato aspermy cucumovirus. Its spherical virions (28 nm in diameter) contained a coat protein of approximately 29 kDa and encapsidated four species of RNAs with similar electrophoretic mobilities to RNAs 1-4 of CMV strains Fny and LS and those of PSV strains J and W. Its encapsidated RNAs hybridized in slot-blot hybridization assay with the complementary DNA probe of PSV-W RNAs but not with those of CMV strains. Therefore, on the basis of biological, serological and physico-biochemical properties, the virus was identified as PSV. No satellite RNA was associated with the virus. This is the first report of PSV in Iran.

Rsv1-Mediated Resistance Against Soybean mosaic virus-N Is Hypersensitive Response-Independent at Inoculation Site, but Has the Potential to Initiate a Hypersensitive Response-like Mechanism

“…Unincorporated nucleotides were removed with Probe Quant TM G-50 microcolumns (Pharmacia, Piscataway, NJ, U.S.A.). RNA denaturation and slot-blot hybridization were performed as described (Bananej et al 1998), except that the Gene Screen Plus transfer membrane (New Life Science Products, Boston, MA, U.S.A) was used and prehybridization and hybridization were performed at 42°C. Hybridization signals were scanned and imaged with a Molecular Dynamics Storm 840 PhosphoImager (Sunnyvale, CA, U.S.A.).…”

Analysis of Coat Protein of Peanut stunt virus Subgroup II Isolates from Alfalfa Fields in West Iran

“…Despite their importance, limited information is available on the occurrence of viruses of the family Bromoviridae in forage legume fields in Iran, although the occurrence of Alfalfa mosaic virus and two cucumoviruses including Cucumber mosaic virus and PSV has been previously reported (Bananej et al. ; Massumi et al. ).…”

Nucleotide sequence analyses of coat protein gene of peanut stunt virus isolates from alfalfa and different hosts show a new tentative subgroup from Iran

“…Nos. KM823526-KM823529) were clustered in a new subgroup, which we tentatively named subgroup V. Bananej et al [3] previously reported an Iranian PSV isolate (PSV-I). Sequence analysis of parts of RNA1 and 2 by Hajimorad et al [12] showed that PSV-I is more closer to the W strain than to the E strain; nevertheless PSV-I could not be placed into subgroup II and consequently was named 'atypical old world' strain of the virus.…”

“…Currently alfalfa is widely grown in different parts of Iran with the exception of three western provinces [30]. PSV was reported for the first time in Iran on the basis of biological, serological and physico-biochemical properties by Bananej et al [3]. Following further characterization of this isolate based on Northern blot hybridization by Hajimorad et al [12], it was placed in subgroup II.…”