Analysis of Coat Protein of Peanut stunt virus Subgroup II Isolates from Alfalfa Fields in West Iran

Abstract

Alfalfa fields in three western provinces of Iran were surveyed for Peanut stunt virus (PSV) during 2011 and 2012. Forty‐seven of 115 samples tested (41%) were infected with PSV. Phylogenetic analysis using coat protein (CP) gene sequences showed that the Iranian isolates belong to the subgroup II of PSV. Pairwise identity analysis revealed four groups representing four phylogenetic subgroups. PSV strains in subgroups III and IV are closely related to each other, as supported by the lowest nucleotide diversity, high pairwise nucleotide identity and high haplotype diversity as evidence of a recent population expansion after a genetic bottleneck. Using the maximum likelihood method, amino acid 86S in the CP gene of the Iranian PSV isolates was found to be under positive selection, although the likelihood ratio test statistics is not significant. This is the first report of the occurrence and phylogenetic relationships of Iranian PSV isolates in west Iran.

Nucleotide sequence analyses of coat protein gene of peanut stunt virus isolates from alfalfa and different hosts show a new tentative subgroup from Iran

“…The CP gene of Iranian PSV isolates from the current study and those of four previously reported Iranian isolates by Pourrahim and Farzadfar [30] (Acc. Nos.…”

“…According to previously established groupings, the 20 Iranian isolates from this study were all clustered in subgroup II. Five previously characterized Iranian PSV isolates [30] also were grouped in this subgroup. Based on pairwise identities ([96% identity) coupled with maximum likelihood phylogenetic, five isolates from the current study together with four previously characterized Iranian isolates (Acc.…”

“…In fact, most authorities generally agree that alfalfa probably originated in Persia (now mainly Iran) [6]. Currently alfalfa is widely grown in different parts of Iran with the exception of three western provinces [30]. PSV was reported for the first time in Iran on the basis of biological, serological and physico-biochemical properties by Bananej et al [3].…”

The Defense Response of Nicotiana benthamiana to Peanut Stunt Virus Infection in the Presence of Symptom Exacerbating Satellite RNA

“…Peanut stunt virus (PSV) is a pathogen of plants, especially legumes, which belongs to genus Cucumovirus [ 1 , 2 , 3 , 4 , 5 ]. The PSV genome consists of three (+)single-stranded RNAs and five open reading frames (ORFs) encoding the 1a and 2a components of the viral replicase, the movement protein, the coat protein (CP), and the 2b protein known as a viral silencing suppressor [ 6 ].…”

Biological and Molecular Characterization of Alfalfa Mosaic Virus Infecting Trumpet Creeper (Campsis radicans) in Iran

“…CMV and PSV, used as positive control, were obtained from our previous studies Farzadfar et al 2013;Pourrahim and Farzadfar 2014). Leaf tissue from the symptomatic plants was used to mechanically inoculate appropriate herbaceous indicator plants including species belonging to Amaranthaceae, Chenopodiaceae, Fabaceae, Lamiaceae and Solanaceae families.…”