Identification and Characterization of Novel Immunomodulatory Bursal-derived Pentapeptide-II (BPP-II)

Feng, Xiuli; Liu, Qing Tao; Cao, Rui; Zhou, Bin; Zhi, Yong; Deng, Wen; Wei, Jian; Qiu, Ya; Wang, Fang Quan; Gu, Jin; Wang, Feng Juan; Zheng, Qi; Ishag, Hassan Zackaria Ali; Chen, Pu Yan

doi:10.1074/jbc.m111.273854

Cited by 11 publications

(16 citation statements)

References 52 publications

(70 reference statements)

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“…The bursa extracts of BF were sequentially separated and purified as described for soluble polypeptide from the bursal sample as described in the previous reported method [16, 17] with some modification. Briefly, the crude extracts of BF from one-month-old avian were obtained by homogenization and centrifugation at 12,000 rpm for 45 min and then ultrafiltered with molecular weight less than 1 kDa at 4°C for 48 h. The obtained sample was purified on a SinoChrom ODS-BP RP-HPLC affinity column (250 × 4.6 mm, Elite) using a linear gradient of acetonitrile (2–100%) with a DAD UV-V detector at 220 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, bursal peptide BP8 could promote colony-forming pre-B formation and regulate B cell development [13], and BP5 regulated B cell development by promoting antioxidant defense [14]. Bursal pentapeptide-II (BPP-II) and BP5 regulated various pathways and immune-related biological processes in hybridoma cells secreting monoclonal antibody especial to JEV [15, 16]. Additionally, bursal pentapeptide-I (BPP-I) inhibited tumor cell proliferation and induced p53 expression [17].…”

Section: Introductionmentioning

confidence: 99%

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The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy

Feng

Zong

Guang

et al. 2019

Journal of Immunology Research

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The bursa of Fabricius is an acknowledged central humoral immune organ unique to birds, which is vital to B cell differentiation and antibody production. However, the function and mechanism of the biological active peptide isolated from bursa on B cell development and autophagy were less reported. In this study, we isolated a new oligopeptide with nine amino acids Leu-Met-Thr-Phe-Arg-Asn-Glu-Gly-Thr from avian bursa following RP-HPLC, MODIL-TOP-MS, and MS/MS, which was named after BP9. The results of immunization experiments showed that mice injected with 0.01 and 0.05 mg/mL BP9 plus JEV vaccine generated the significant increased antibody levels, compared to those injected with JEV vaccine only. The microarray analysis on the molecular basis of BP9-treated immature B cell showed that vast genes were involved in various immune-related biological processes in BP9-treated WEHI-231 cells, among which the regulation of cytokine production and T cell activation were both major immune-related processes in WEHI-231 cells with BP9 treatment following network analysis. Also, the differentially regulated genes were found to be involved in four significantly enriched pathways in BP9-treated WEHI-231 cells. Finally, we proved that BP9 induced the autophagy formation, regulated the gene and protein expressions related to autophagy in immature B cell, and stimulated AMPK-ULK1 phosphorylation expression. These results suggested that BP9 might be a strong bursal-derived active peptide on antibody response, B cell differentiation, and autophagy in immature B cells, which provided the linking among humoral immunity, B cell differentiation, and autophagy and offered the important reference for the effective immunotherapeutic strategies and immune improvement.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy

Feng

Zong

Guang

et al. 2019

Journal of Immunology Research

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“…The BF contains a variety of bioactive substances, and bursin, the first peptide isolated from BF, selectively stimulated the differentiation of avian B lymphocytes precursor in vitro [ 3 5 ]. In recent years, various peptides have been isolated from BF, most of which have immunoregulatory functions [ 7 8 9 10 11 17 20 23 ]. In addition to their immune regulatory function, bursal peptides (BPs) derived from BF have exhibited other biological functions.…”

Section: Introductionmentioning

confidence: 99%

Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine

et al. 2018

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The bursa of Fabricius (BF) is a central humoral immune organ unique to birds. Four bursal peptides (BP-I, BP-II, BP-III, and BP-IV) have been isolated and identified from the BF. In this study, the immunoadjuvant activities of BPs I to IV were examined in mice immunized with H9N2 avian influenza virus (AIV) vaccine. The results suggested that BP-I effectively enhanced cell-mediated immune responses, increased the secretion of Th1 (interferon gamma)- and Th2 (interleukin-4)-type cytokines, and induced an improved cytotoxic T-lymphocyte (CTL) response to the H9N2 virus. BP-II mainly elevated specific antibody production, especially neutralizing antibodies, and increased Th1- and Th2-type cytokine secretion. BP-III had no significant effect on antibody production or cell-mediated immune responses compared to those in the control group. A strong immune response at both the humoral and cellular levels was induced by BP-IV. Furthermore, a virus challenge experiment followed by H&E staining revealed that BP-I and BP-II promoted removal of the virus and conferred protection in mouse lungs. BP-IV significantly reduced viral titers and histopathological changes and contributed to protection against H9N2 AIV challenge in mouse lungs. This study further elucidated the immunoadjuvant activities of BPs I to IV, providing a novel insight into immunoadjuvants for use in vaccine design.

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“…It plays a central role in the proliferation and differentiation of the antibody-producing B lymphocyte lineage1. Previous studies have shown that some defined peptides sequenced from a BF extract (e.g., bursin and bursal anti-steroidogenic peptide) stimulate specific immune cell subsets2,3 isolated a new pentapeptide, bursopentin (BP5; Cys-Lys-Arg-Val-Tyr), from a BF extract. BP5 was shown to have immunomodulatory effects, including effects on T and B cells, the antioxidant stress response of macrophages, and an inhibitory effect on tumor cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have focused on BF extracts and multiple biologically active factors and their effects on the humoral immune system in chickens and other birds2. However, the mechanisms through which these immunomodulatory peptides affect B lineage cell proliferation and antibody production in chickens is poorly understood.…”

Section: Introductionmentioning

confidence: 99%

The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells

Luo

Feng

et al. 2018

Afr H. Sci.

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BackgroundIn the recent past, many studies have been focused on extracts of BF and multiple biologically active factors and their effects on humoral immune system in chickens and birds. However, the mechanism of those immunomodulatory peptides on the B lineage cells proliferation and antibody production in chicken is fairly unknown. DT40 cell line, an avian leucosis virus-induced chicken pre-B cell line, expresses immunoglobulin M (IgM) isotype B cell reporter in the plasma membrane. There are many evidences suggesting that DT40 cells are best characterized as a bursal stem cell line. Because of the unique characteristics of DT40 cell line, it has been widely used to observe biological processes of pre-B lymphocyte cell within living cells.MethodsThe chicken B cell line DT40 was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium and cytotoxicity was studied. Also, effect of BP5 on cell proliferation and cell cycle distribution of DT40 cells was studied. Also, the effect of BP5 on sIgM mRNA expression was studied by using real-time PCR.ObjectivesTo investigat the effects of Bursopentin (Cys-Lys-Arg-Val-Tyr, BP5) on a chicken promyelocyte cell line DT40, assays of cell proliferation, cell cycle distribution, detection of surface immunoglobulin G (sIgM) mRNA expression and gene microarray analysis were performed.ResultsThe results showed that BP5 displayed concentration-dependent effects on the proliferation, cell cycle, and sIgM mRNA expression in DT40 cells. And the analysis of expression profiles identified a signature set of 3022 genes (1254 up regulated genes, 1762 down regulated genes), which clearly discriminated the BP5-treated DT40 cells from control with high certainty (P≤0.02). The results of microarray analysis were confirmed by quantitative reverse transcription-polymerase chain reaction for 12 of the differentially expressed genes.ConclusionTheses findings showed the immuno-activity effect of BP5 on B lymphocyte and indicated that BP5 treatment regulated eight signaling pathways, in which Toll-like signaling pathway was the most significant enrichment pathway.

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Identification and Characterization of Novel Immunomodulatory Bursal-derived Pentapeptide-II (BPP-II)

Cited by 11 publications

References 52 publications

The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy

The Functions and Mechanism of a New Oligopeptide BP9 from Avian Bursa on Antibody Responses, Immature B Cell, and Autophagy

Comparison of immunoadjuvant activities of four bursal peptides combined with H9N2 avian influenza virus vaccine

The immunomodulatory peptide bursopentin (BP5) enhances proliferation and induces sIgM expression in DT40 cells

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