Identification and characterization of microsatellite markers in Hermann's tortoise (<i>Testudo hermanni</i>, Testudinidae)

Forlani, Anna; Crestanello, Barbara; Mantovani, Sara; Livoreil, Barbara; Zane, Lorenzo; Bertorelle, Giorgio; Congiu, Leonardo

doi:10.1111/j.1471-8286.2005.00890.x

Cited by 29 publications

(20 citation statements)

References 6 publications

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“…From this, 15 polymorphic markers were selected for genetic analysis (Zenboudji et al, in prep). We added two polymorphic microsatellite loci (Test10, Test56) characterized for T. hermanni by Forlani et al (2005). The 17 microsatellite loci were amplified and sequenced in six multiplexes (Zenboudji et al, in prep).…”

Section: Microsatellite Analysismentioning

confidence: 99%

Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography

Zenboudji

Cheylan

Arnal

et al. 2016

Biological Conservation

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Estimating the genetic variation and demographic trends of species in decline is of major concern in conservation genetics. This study contributes to understanding how historical and anthropogenic factors shape the distribution of current genetic diversity in one of the most endangered reptiles in Western Europe, the Hermann's tortoise Testudo hermanni hermanni. We used 17 microsatellite loci, chosen from a pyrosequencing library specifically developed for the subspecies to genotype eight populations distributed over about 30 sample localities covering almost the entire geographic distribution of the sub-species. The population genetic results reflect a very strong genetic structure and identify three major clusters among the Hermann's tortoise in the occidental Mediterranean basin: a continental cluster (Albera in Spain, Var in France and continental Italy), an insular cluster (Corsica, Sardinia and Sicily) and a cluster of mixed lineage (Minorca). Each of the eight studied populations is characterized by only one lineage except for Minorca, in which two lineages were identified. In contrast to what most empirical studies predict, the genetic diversity of the Hermann's tortoise is greater on islands than on the continent. Islands can therefore be considered as genetic sanctuaries with high conservation potential for this species, particularly in Corsica. Historical demographic patterns inferred with a generalized stepwisemutation model (GSM) using maximum likelihood showed significant past demographic changes in only two of the eight sampled populations: a demographic bottleneck was detected in the Albera population (Spain) and a demographic expansion in Corsica (France). In contrast to what was argued in previous studies, these results indicate that the Hermann's tortoise lineage found in Corsica is autochthonous. The origin of both lineages found in Minorca remains speculative. Lastly, our study identifies the six most relevant management units (sensu Moritz, 1994) for conservation purposes on the basis that they represent a significant part of the evolutionary legacy of the species. Some conservation recommendations were proposed, in particular for the most threatened population in Albera.

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Section: Microsatellite Analysismentioning

confidence: 99%

Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography

Zenboudji

Cheylan

Arnal

et al. 2016

Biological Conservation

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“…Twelve microsatellite loci were analysed for each tortoise following Salinas et al (2010): Goag5, Goag6 (designed for Gopherus agassizii; Edwards et al, 2003), GmuB08, GmuD16, GmuD51 (designed for Glyptemys muhlenbergii; King and Julian, 2004), GP61, GP81 (designed for Gopherus polyphemus; Schwartz et al, 2003), Test10, Test21, Test56, Test71, and Test76 (designed for Testudo hermanni; Forlani et al, 2005). Microsatellite loci were individually PCR-amplified in a final volume of 25 μl using 1 unit Taq polymerase (Bioron, Ludwigshafen, Germany) with the buffer recommended by the supplier and a final concentration of 1.6 mM MgCl 2 (Bioron), 0.2 mM of ; table 2) and approximately 10-40 ng of total DNA.…”

Section: Sampling Dna Extraction Pcr Fragment Analysis and Sequencingmentioning

confidence: 99%

Is the imperilled spur-thighed tortoise (Testudo graeca) native in Sardinia? Implications from population genetics and for conservation

Vamberger¹,

Corti

Stuckas³

et al. 2011

Amphib Reptilia

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Using mtDNA sequences and 12 microsatellite loci, we compare populations of Testudo graeca from Sardinia and North Africa. The observed pattern of almost no differentiation combined with reduced variation in the Sardinian population is consistent with introduction in prehistoric or historic times from what is now Tunisia and neighbouring Algeria. Furthermore, in the light of the recently published recommendation to eradicate the non-native T. graeca from Italy, we review recent studies on the archaeological and fossil record, on the phylogeography and population genetics of the three other chelonian species occurring in Sardinia (Emys orbicularis, T. hermanni, T. marginata). We conclude that the extant Sardinian populations of all four species are not native. However, they are and should be safeguarded under EC law (Council Regulation No 338/97 on the Protection of Species of Wild Fauna and Flora; Flora Fauna Habitat Directive: Appendix IV, Art. 12) because they serve as a back-up for the declining mainland populations. Moreover, these populations constitute an important part of the human-shaped natural heritage of the Mediterranean.

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“…PCR products were cloned in Pgem T Easy Vector System I (Promega). About 200 recombinant colonies were screened by PCR with universal M13 primers and dot blot hybridization as reported in Forlani et al (2005). Among the ones positive to the hybridization, 40 clones were sequenced and 22 contained a microsatellite motif and adequate flanking regions for primer designing.…”

mentioning

confidence: 99%

Isolation of microsatellite loci from the endemic and endangered Adriatic sturgeon (Acipenser naccarii)

2007

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With the aim of elaborating a breeding plan on a captive stock of the highly endangered Adriatic sturgeon (Acipenser naccarii), a total of 10 polymorphic microsatellite loci were isolated from an enriched library. The results of cross amplification of additional 8 loci previously isolated from A. oxyrinchus, A. fulvescens and Scaphyrinchus platorhynchus are also reported. Given the tetraploid condition of the species the genetic variability was estimated basing on the number of alleles per individuals and the average band sharing.

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Identification and characterization of microsatellite markers in Hermann's tortoise (Testudo hermanni, Testudinidae)

Cited by 29 publications

References 6 publications

Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography

Conservation of the endangered Mediterranean tortoise Testudo hermanni hermanni: The contribution of population genetics and historical demography

Is the imperilled spur-thighed tortoise (Testudo graeca) native in Sardinia? Implications from population genetics and for conservation

Isolation of microsatellite loci from the endemic and endangered Adriatic sturgeon (Acipenser naccarii)

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