Identification and characterization of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian companion animals and horses

Loncaric, Igor; Künzel, Frank; Licka, Theresia; Simhofer, Hubert; Spergser, Joachim; Rosengarten, Renate

doi:10.1016/j.vetmic.2013.11.022

Cited by 70 publications

(64 citation statements)

References 38 publications

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“…In short, they carried PVL genes as well as blaZ , aacA-aphD , aadD and dfrS1 . CC22 is a common “human lineage” but has also been found in companion animals with close contacts to humans [125,126] and in wild roe deer [11]. CC22 MRSA are extremely common in Western Europe, but the rook isolates were not identical to the ubiquitous Barnim/UK-EMRSA-15 epidemic strain differing in SCC mec subtype (IVc rather than IVh; [77]) and presence of PVL genes.…”

Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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Staphylococcus aureus is a well-known colonizer and cause of infection among animals and it has been described from numerous domestic and wild animal species. The aim of the present study was to investigate the molecular epidemiology of S. aureus in a convenience sample of European wildlife and to review what previously has been observed in the subject field. 124 S. aureus isolates were collected from wildlife in Germany, Austria and Sweden; they were characterized by DNA microarray hybridization and, for isolates with novel hybridization patterns, by multilocus sequence typing (MLST). The isolates were assigned to 29 clonal complexes and singleton sequence types (CC1, CC5, CC6, CC7, CC8, CC9, CC12, CC15, CC22, CC25, CC30, CC49, CC59, CC88, CC97, CC130, CC133, CC398, ST425, CC599, CC692, CC707, ST890, CC1956, ST2425, CC2671, ST2691, CC2767 and ST2963), some of which (ST2425, ST2691, ST2963) were not described previously. Resistance rates in wildlife strains were rather low and mecA-MRSA isolates were rare (n = 6). mecC-MRSA (n = 8) were identified from a fox, a fallow deer, hares and hedgehogs. The common cattle-associated lineages CC479 and CC705 were not detected in wildlife in the present study while, in contrast, a third common cattle lineage, CC97, was found to be common among cervids. No Staphylococcus argenteus or Staphylococcus schweitzeri-like isolates were found. Systematic studies are required to monitor the possible transmission of human- and livestock-associated S. aureus/MRSA to wildlife and vice versa as well as the possible transmission, by unprotected contact to animals. The prevalence of S. aureus/MRSA in wildlife as well as its population structures in different wildlife host species warrants further investigation.

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Section: Resultsmentioning

confidence: 99%

Diversity of Staphylococcus aureus Isolates in European Wildlife

et al. 2016

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“…This method involves PCR amplification of five specific loci (sdr, clfA, clfB, ssp and spa) of S. aureus which is composed of seven individual genes (sdrCDE, clfA, clfB, sspA, spa, mecA and fnbP) [35] using multiplex PCR mixture followed by separation of the amplified bands on agarose gel and comparison of the band patterns between strains to identify genetic clusters or clones [36]. This genotyping method showed a successful typing of MRSA isolates in many studies [37], in term of determining the genetic diversity and evolutionary lineage with discriminatory power.…”

Section: Multiple-locus Variable-number Tandem Repeat Assay (Mlva)mentioning

confidence: 99%

Genotyping Approaches for Identification and Characterization of Staphylococcus aureus

Al-Obaidi¹,

Suhaili²,

MohdDesa³

2018

Genotyping

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Genotyping methods are vital epidemiological tools for discriminating different bacterial isolates within same species, which in turn provide useful data in tracing source of infection and disease management. There have been a revolutionary efforts in ways to distinguish between bacterial types and subtypes at molecular level utilizing DNA in the genomes. Notably, the growth of various DNA typing methods has provided innovative apparatuses for improved surveillance and outbreak investigation. Thus, early identification and genotyping are indispensable as resources for managing therapeutic treatment and the control of rapid expansion of clinically important bacteria. Methicillin-resistant Staphylococcus aureus (MRSA) has been in a great attention due to its contagious nature and subjected to various typing analyses. Thus, in this chapter, we aimed to review the contribution of various genotyping methods of commonly used as well as those unique to staphylococci in understanding its epidemiology, infection and dissemination pattern, and to provide an impression of specific advantages and disadvantages of each tool.

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“…On the other hand, MRSA isolated in Portugal and Thailand from dogs and Germany from cats possessed a lorfenicol-chloramphenicol exporter gene, fexA, that mediates resistance to both luorinated and nonluorinated phenicols [205][206][207]. MRSA ST398 isolated from an Austrian dog sufering from vaginitis harbored the vatC gene that inactivates streptogramin A [208]. An ABC transporter gene, lsaE (responsible…”

Section: Canine and Feline Mrsamentioning

confidence: 99%

“…Multidrugresistant ST8 MRSA was detected in Belgium, Germany, Swizerland, and the USA. A single locus variant of ST8 classiied as ST254 MRSA was isolated from horses in Austria, Germany, Ireland, and the UK [183,208,222,223]. Twelve diferent MLST types have been reported and most of the MRSA strains were grouped into the CC8 or CC398 classes [224].…”

Section: Equine Mrsamentioning

confidence: 99%

Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

Chon¹,

Sung²,

Khan³

2017

Frontiers in Staphylococcus Aureus

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Methicillin-resistant Staphylococcus aureus (MRSA) has become a growing concern in companion and food-producing animals. The presence of multidrug-resistance with a wide range of extracellular enterotoxin genes, virulence factors, and Panton-Valentine leukocidin (pvl) cytotoxin genes confer life-threatening traits on MRSA and makes them highly pathogenic and diicult to treat. Clonal complex 398 (CC398), a predominant clonal lineage of livestock-associated-MRSA in domestic animals and retail meat, is capable of infecting humans. In order to monitor and prevent MRSA contamination, it is critical to understand its source and transmission dynamics. In this review, we describe MRSA in food-producing animals (pig, catle, chicken), horses, pet animals (dogs, cats), and food products (pork, beef, chicken, milk, and ish).

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Identification and characterization of methicillin-resistant Staphylococcus aureus (MRSA) from Austrian companion animals and horses

Cited by 70 publications

References 38 publications

Diversity of Staphylococcus aureus Isolates in European Wildlife

Diversity of Staphylococcus aureus Isolates in European Wildlife

Genotyping Approaches for Identification and Characterization of Staphylococcus aureus

Methicillin-Resistant Staphylococcus aureus (MRSA) in Food- Producing and Companion Animals and Food Products

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