Identification and characterization of MAVS from black carp Mylopharyngodon piceus

Zhou, Wei; Zhou, Jain; Lv, Ying; Qu, Yixiao; Chi, Mengdie; Li, Jun; Feng, Hao

doi:10.1016/j.fsi.2015.01.016

Cited by 60 publications

(21 citation statements)

References 39 publications

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“…HEK293T (293T), HeLa, Ctenopharyngodon idella kidney (CIK) and epithelioma papulosum cyprinid (EPC) cells are maintained in our laboratory [37,39]. Mylopharyngodon piceus fin (MPF) cells were a kind gift from Dr. Tiansheng Chen (Huazhong Agricultural University).…”

Section: Cells and Plasmidsmentioning

confidence: 99%

“…The transfected cells were harvested at 48 h posttransfection and lysed for the immunoblotting (IB), assay as previously described [37]. Briefly, whole-cell lysates were isolated with 10% SDS-PAGE and transferred a membrane, and the membrane was probed with mouse monoclonal anti-HA antibody (1:3000; Sigma).…”

Section: Immunoblottingmentioning

confidence: 99%

“…In a previous study, we identified one type I IFN of black carp (bcIFNa) as a secreted antiviral cytokine directed against SVCV and GCRV, and suggested that it plays an important role in the host's innate immune response [36]. MAVS (bcMAVS) and IKKε (bcIKKε) of the black carp activated the promoter activity of zebrafish IFN and fathead minnow IFN, whose count-parts in higher vertebrates are upstream positive regulators of IFNs [37,38]. To clarify the mechanism underlying the antiviral activity of bcIFNa, Mx1 of black carp (bcMx1) was cloned and characterized in this study.…”

Section: Introductionmentioning

confidence: 99%

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Mx1 of black carp functions importantly in the antiviral innate immune response

Xiao

Yan

Hui

et al. 2016

Fish & Shellfish Immunology

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Section: Cells and Plasmidsmentioning

confidence: 99%

Section: Immunoblottingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Mx1 of black carp functions importantly in the antiviral innate immune response

Xiao

Yan

Hui

et al. 2016

Fish & Shellfish Immunology

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“…It has a long sex maturation period, namely 5-7 years in males and 7-9 years in females (Rothbard et al, 1997). Studies on black carp have mainly focused on artificial breeding (Hosain et al, 2015), aquaculture (Hu et al, 2015) and immunity (Zhou et al, 2015). Black carp has suffered infections by pathogenic microorganisms such as spring viremia of carp virus (SVCV) (Xiao et al, 2016), although investigations underlying its pathological mechanisms are limited.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a cell line from the kidney of black carp and its susceptibility to spring viremia of carp virus

Xue

Wang

Pan

et al. 2017

Journal of Fish Diseases

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In this study, we established and characterized a cell line derived from the kidney of black carp (Mylopharyngodon piceus), which is an important freshwater aquaculture species. The cell line was designated as MPK and subcultured for more than 70 passages in DMEM medium containing 10% fetal bovine serum (FBS) at 28°C. MPK had a modal diploid chromosome number of 48. Moreover, a transient MPK transfection efficiency was up to 18% using a green fluorescent protein plasmid by a modified electroporation. In addition, the MPK cells showed susceptibility to spring viremia of carp virus (SVCV), as demonstrated by the presence of severe cytopathic effects (CPEs) and increased viral RNA. Unexpectedly, the MPK cells expressed pluripotency-associated genes such as nanog, oct4 and vasa, indicating that these are possibly adult stem cells. Taken together, we have established a stable cell line from kidney that may potentially be utilized as an in vitro platform for genetic modifications and host-pathogen analysis in black carp.

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“…HEK293T, Epithelioma papulosum cyprini (EPC), Ctenopharyngodon idella kidney (CIK) and Mylopharyngodon piceus fin (MPF) cells were kept in the lab (Zhou et al, 2015). HEK293T cells were cultured at 37°C with 5% CO 2 ; EPC, CIK and MPF cells were cultured at 25°C with 5% Table 1 Primers used in the study.…”

Section: Cells and Plasmidsmentioning

confidence: 99%

TRAF3 enhances STING-mediated antiviral signaling during the innate immune activation of black carp

Wang

Song

Xie

et al. 2018

Developmental & Comparative Immunology

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Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a main regulator of antiviral and anti-inflammatory pathways in mammals, which is considered to induce type I interferon (IFN) activation and negatively regulate the activation of the canonical and non-canonical NF-κB pathways. To elucidate its function in teleost fish, TRAF3 homologue of black carp (Mylopharyngodon piceus) has been cloned and characterized in this study. The open reading frame (ORF) of black carp TRAF3 (bcTRAF3) consists of 1722 nucleotides and bcTRAF3 contains 574 amino acids. bcTRAF3 protein migrated around 65 KDa in immunoblot analysis of both EPC and HEK293T cells. bcTRAF3 was identified as a cytosolic protein and suggested to form aggregates or be associated with vesicles scattering in the cytoplasm. It was interesting that both NF-κB and IFN transcription was activated by bcTRAF3 in reporter assay. When co-expressed with black carp STING (bcSTING), bcTRAF3 was redistributed in the cytoplasm and its subcellular location overlapped with that of bcSTING no matter what the cells was infected with GCRV or not, which suggested the association between these two molecules. bcSTING-mediated IFN production was up-regulated by bcTRAF3 in a dose dependent manner in reporter assay. Accordingly, EPC cells transfected with both bcSTING and bcTRAF3 showed enhanced antiviral activity comparing EPC cells expressing bcSTING alone. Taken together, the data generated in this paper supported the conclusion that bcTRAF3 was recruited into host innate immune activation and positively regulated bcSTING-mediated antiviral signaling.

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Identification and characterization of MAVS from black carp Mylopharyngodon piceus

Cited by 60 publications

References 39 publications

Mx1 of black carp functions importantly in the antiviral innate immune response

Mx1 of black carp functions importantly in the antiviral innate immune response

Establishment of a cell line from the kidney of black carp and its susceptibility to spring viremia of carp virus

TRAF3 enhances STING-mediated antiviral signaling during the innate immune activation of black carp

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