Identification and characterization of <i>TSAP</i>, a novel gene specifically expressed in testis during spermatogenesis

Li, Bin; Wu, Gang; Hu, Jian; Gong, Wuming; Yue, Mingjin; Song, Ping

doi:10.1002/mrd.20679

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…Three gene models are currently annotated within this region ( Potefam3f , Potefam3a , and Potefam3b ), all of which encode proteins containing ankyrin repeats and annotated as members of the POTE ankyrin domain 3 family. The expression pattern of one member of this family, named Potefam3 , was previously characterized as postreplicative male germ cell specific ( Bin et al 2007 ), but function was not determined. Our newly identified genes do not show any homology with this gene family but instead show similarity to another gene model, Gm53405 , ranging from 76%–90% over around one-third of the protein length ( Supplemental Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

Gill,

Rohmer,

Erkek-Ozhan

et al. 2023

Genome Res.

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In mammals, the adult testis is the tissue with the highest diversity in gene expression. Much of that diversity is attributed to germ cells, primarily meiotic spermatocytes and postmeiotic haploid spermatids. Exploiting a newly developed cell purification method, we profiled the transcriptomes of such postmitotic germ cells of mice. We used a de novo transcriptome assembly approach and identified thousands of novel expressed transcripts characterized by features distinct from those of known genes. Novel loci tend to be short in length, monoexonic, and lowly expressed. Most novel genes have arisen recently in evolutionary time and possess low coding potential. Nonetheless, we identify several novel protein-coding genes harboring open reading frames that encode proteins containing matches to conserved protein domains. Analysis of mass-spectrometry data from adult mouse testes confirms protein production from several of these novel genes. We also examine overlap between transcripts and repetitive elements. We find that although distinct families of repeats are expressed with differing temporal dynamics during spermatogenesis, we do not observe a general mode of regulation wherein repeats drive expression of nonrepetitive sequences in a cell type–specific manner. Finally, we observe many fairly long antisense transcripts originating from canonical gene promoters, pointing to pervasive bidirectional promoter activity during spermatogenesis that is distinct and more frequent compared with somatic cells.

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Section: Resultsmentioning

confidence: 99%

De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

Gill,

Rohmer,

Erkek-Ozhan

et al. 2023

Genome Res.

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Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development

Yang

et al. 2009

Mol Biol Rep

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During the process of screening genes that are preferentially expressed in zebrafish germ cells, we isolated an E2F family member zE2F5b, which is a truncated isoform of zebrafish E2F5a. The E2F transcription factors are thought to play an essential role in cell cycle progression. Reverse transcription polymerase chain reaction and whole mount in situ hybridization analyses have demonstrated that both zE2F5a and zE2F5b mRNAs are predominantly expressed in stage I and II oocytes. In early development, the two zE2F5 transcripts are expressed by many parts of the embryo and are then restricted to the brain area. Interestingly, zE2F5b mRNA is detected later in the lens and optic tectum while zE2F5a mRNA is absent. Our data suggests that E2F5 may play important roles in zebrafish brain, eye and ovarian development.

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TSEG-1, a novel member of histone H2A variants, participates in spermatogenesis via promoting apoptosis of spermatogenic cells

Tong

Zheng

et al. 2010

Genomics

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A novel variant of histone H2A, named as testis specific expressed gene 1 (TSEG-1, approved symbol: H2afb1), was identified from adult mouse testis. The TSEG-1 gene is 610-bp in length and consists of one exon. TSEG-1 transcript was robustly and exclusively expressed in adult mouse testis, mainly in spermatocytes. In developmental testis, the TSEG-1 transcript was robustly expressed since postnatal day (P) 21, peaked at P30, and gradually decreased in the testis of aging mouse. The surgical cryptorchidism mouse model showed an increase in the TSEG-1 expression, accompanied by enhanced apoptosis of spermatogenic cells. The EGFP-tagged TSEG-1 protein is located in the nuclei of cultured spermatocytes (GC-2spd cells). Transfection of TSEG-1 into GC-2spd cells resulted in suppressed cell viabilities, increased apoptosis, and decreased mitochondrial membrane potential. Intratesticular injection of TSEG-1 resulted in increased apoptosis of spermatogenic cells in vivo. These results suggest that TSEG-1 may participate in the spermatogenesis via regulating the apoptosis of spermatogenic cells.

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Identification and characterization of TSAP, a novel gene specifically expressed in testis during spermatogenesis

Cited by 3 publications

References 33 publications

De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

De novo transcriptome assembly of mouse male germ cells reveals novel genes, stage-specific bidirectional promoter activity, and noncoding RNA expression

Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development

TSEG-1, a novel member of histone H2A variants, participates in spermatogenesis via promoting apoptosis of spermatogenic cells

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