Identification and expression analysis of two zebrafish E2F5 genes during oogenesis and development

Abstract

During the process of screening genes that are preferentially expressed in zebrafish germ cells, we isolated an E2F family member zE2F5b, which is a truncated isoform of zebrafish E2F5a. The E2F transcription factors are thought to play an essential role in cell cycle progression. Reverse transcription polymerase chain reaction and whole mount in situ hybridization analyses have demonstrated that both zE2F5a and zE2F5b mRNAs are predominantly expressed in stage I and II oocytes. In early development, the two zE2F5 transcripts are expressed by many parts of the embryo and are then restricted to the brain area. Interestingly, zE2F5b mRNA is detected later in the lens and optic tectum while zE2F5a mRNA is absent. Our data suggests that E2F5 may play important roles in zebrafish brain, eye and ovarian development.

Differential Proteomics Analysis Reveals a Role for E2F2 in the Regulation of the Ahr Pathway in T Lymphocytes

“…The list of functions in which E2Fs are involved is probably not complete. For instance, a role for E2F in development has been proposed recently in Xenopus and zebrafish (4,5), and there is also evidence for their role in processes such as endocycle regulation and plant cell size control (6,7), or in autophagy (8).…”

Cellular Factors Involved in Transcriptome Dynamics in Early Zebrafish Embryogenesis

“…One of the two uniquely enriched motifs in the non-expressed downstream set is Pou5f1::Sox2, a protein that is implicated in the maintenance of pluripotency, the activation of zygotic genes and in the regulation of miR-430, a miRNA responsible for the clearance of maternal mRNAs [23]. E2F TFs are involved in regulation of cell cycle progression [24].…”