Identification and characterization of glutathione S-transferases and their potential roles in detoxification of abamectin in the rice stem borer, Chilo suppressalis

Meng, Xiangkun; Wu, Zhaolu; Jiang, Chengyun; Guan, Daojie; Zhang, Nan; Jiang, Heng; Shen, Qinwen; Qian, Kun; Wang, Jianjun

doi:10.1016/j.pestbp.2022.105050

Cited by 13 publications

(21 citation statements)

References 45 publications

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“…(2) involve in the detoxification metabolism of insecticides through the non-catalytic binding to the insecticides or directly metabolism of insecticide (Meng et al, 2022;Zhou et al, 2013). Previous studies reported that the expression of GST genes was increasing along with the ageing in some insects, such as D. melanogaster, Apis mellifera and C. pomonella (Liu et al, 2014, p. 1;Papadopoulos et al, 2004;Zou et al, 2000).…”

Section: Sequence Characteristics Of Six Gmgst Genes Includingmentioning

confidence: 99%

“…In Bactrocera dorsalis , both qRT‐PCR and Western blot results demonstrated that BdGSTe8 expressed higher in resistant strain than in sensitive strain of malathion (Lu et al, 2020). In Chilo suppressalis , the susceptibility of larvae to abamectin was significantly increased when using diethyl maleate (DEM) to inhibited GST enzyme activity (Meng et al, 2022). However, very few studies have been carried out about G. molesta GST genes associated with insecticide susceptibility; only Guo et al, 2017 identified the GST genes in G. molesta through transcriptome analysis (Guo et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Zhang

Jia

et al. 2023

J Applied Entomology

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Grapholita molesta is one kind of serious fruit‐boring pests in the world, which caused huge economic losses to the fruit industry including pear and apple. The heavy usage of synthetic insecticides resulted in different degrees of resistance of G. molesta to some commonly used insecticides. However, the tolerance mechanisms of G. molesta to insecticides are still unclear. Glutathione S‐transferases (GSTs) belong to the superfamily of detoxifying enzymes and mainly play important roles in detoxification metabolism and insecticide susceptibility of insects. In this study, we first evaluated the toxicity of three insecticides including emamectin benzoate, chlorantraniliprole and lambda‐cyhalothrin on G. molesta at different developmental stages. Synergism study showed that the susceptibility of G. molesta larvae to these three insecticides obviously increased when the enzyme activity of GST was inhibited by diethyl maleate compared with the controls. Next, we screened out six GmGST genes which are upregulated after insecticides treatment from a total of 21 GmGST genes and analysed their sequence characteristics and expression profiles. RNAi and bioassay results further revealed that the mortality of 5th instar larvae significantly increased after exposure to LC30 of emamectin benzoate, chlorantraniliprole and lambda‐cyhalothrin when silencing of GmGSTo2, GmGSTs1 and GmGSTz1 compared with the controls. In conclusion, this study indicated that GmGSTo2, GmGSTs1 and GmGSTz1 play important roles in insecticides susceptibility of G. molesta to three commonly used insecticides, which is significance for its future integrated management.

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Section: Sequence Characteristics Of Six Gmgst Genes Includingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Zhang

Jia

et al. 2023

J Applied Entomology

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“…Glutathione S-transferases (GSTs) were also found to exert crucial influence in promoting the detoxification against natural and synthetic xenobiotics for pest control and are related to the evolution of resistance to chemical agents in insect pests [ 8 ]. Metabolic assays indicated that the activity of GST in two abamectin-resistant populations of the vegetable leafminer, Liriomyza sativae , was greatly increased compared with the susceptible population, suggesting that enhanced activity of GST could be one primary factor conferring resistance to abamectin in this pest insect [ 9 ], and recently it was found that in Chilo suppressalis , the rice borer, inhibited activity of GST markedly elevated the susceptibility to abamectin [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China

Wang

Fang

Che

et al. 2022

Toxins

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Abamectin, produced by the soil-dwelling actinomycete Streptomyces avermitilis, belongs to the macrocyclic lactones class of pesticides, has nematocidal, acaricidal, and insecticidal activity, and is highly effective when used against targeted species. Bemisia tabaci, the tobacco whitefly, is a highly destructive insect to agricultural production worldwide, and various insecticide-resistant strains have been identified in China. Here, we monitored levels of resistance to abamectin in twelve field-collected B. tabaci populations from northern China, and confirmed that, compared with the lab reference strain, six field populations exhibited strong abamectin resistance, while the other six exhibited low-to-medium resistance. Among these, the Xinzheng (XZ) population displayed about a 40-fold increased resistance to abamectin, and experienced significant cross-resistance to chlorpyrifos and imidacloprid. The abamectin resistance of XZ was found to be autosomal and incompletely dominant. Metabolic enzyme and synergism tests were conducted, and two metabolic enzymes, glutathione S-transferase and P450 monooxygenase, were found to be conducive to the field-developed abamectin resistance of the XZ population. The above results provide valuable information that can be used in identifying new pest control strategies and delaying the evolution of resistance to abamectin in field populations of whiteflies.

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“…Knowledge of resistance mechanisms in P. xylostella against various insecticides is very important in its management [ 57 , 58 ]. The resistance is a function of increased activity of detoxification enzymes, insensitivity of the target site, knockdown resistance, and diminished cuticular penetration [ 59 ].…”

Section: Resultsmentioning

confidence: 99%

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

Bilal

Sial

Cao

et al. 2022

IJMS

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The diamond back moth, Plutella xylostella, causes severe damage at all crop stages, beside its rising resistance to all insecticides. The objective of this study was to look for a new control strategy such as application of insecticide-loaded carbon dot-embedded fluorescent mesoporous silica nanoparticles (FL-SiO2 NPs). Two different-sized methoxyfenozide-loaded nanoparticles (Me@FL-SiO2 NPs-70 nm, Me@FL-SiO2 NPs-150 nm) were prepared, with loading content 15% and 16%. Methoxyfenozide was released constantly from Me@FL-SiO2 NPs only at specific optimum pH 7.5. The release of methoxyfenozide from Me@FL-SiO2 NPs was not observed other than this optimum pH, and therefore, we checked and controlled a single release condition to look out for the different particle sizes of insecticide-loaded NPs. This pH-responsive release pattern can find potential application in sustainable plant protection. Moreover, the lethal concentration of the LC50 value was 24 mg/L for methoxyfenozide (TC), 14 mg/L for Me@FL-SiO2 NPs-70 nm, and 15 mg/L for Me@FL-SiO2 NPs-150 nm after 72 h exposure, respectively. After calculating the LC50, the results predicted that Me@FL-SiO2 NPs-70 nm and Me@FL-SiO2 NPs-150 nm exhibited better insecticidal activity against P. xylostella than methoxyfenozide under the same concentrations of active ingredient applied. Moreover, the activities of detoxification enzymes of P. xylostella were suppressed by treatment with insecticide-loaded NPs, which showed that NPs could also be involved in reduction of enzymes. Furthermore, the entering of FL-SiO2 NPs into the midgut of P. xylostella was confirmed by confocal laser scanning microscope (CLSM). For comparison, P. xylostella under treatment with water as control was also observed under CLSM. The control exhibited no fluorescent signal, while the larvae treated with FL-SiO2 NPs showed strong fluorescence under a laser excitation wavelength of 448 nm. The reduced enzyme activities as well as higher cuticular penetration in insects indicate that the nano-based delivery system of insecticide could be potentially applied in insecticide resistance management.

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Identification and characterization of glutathione S-transferases and their potential roles in detoxification of abamectin in the rice stem borer, Chilo suppressalis

Cited by 13 publications

References 45 publications

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Molecular identification of glutathione S‐transferase genes and their potential roles in insecticides susceptibility of Grapholita molesta

Metabolic Resistance in Abamectin-Resistant Bemisia tabaci Mediterranean from Northern China

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

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