Identification and characterization of chemosensory genes in the antennal transcriptome of Spodoptera exigua

Du, Lixiao; Liu, Yang; Zhang, Jin; Gao, Xiwu; Wang, Bing; Wang, Guirong

doi:10.1016/j.cbd.2018.05.001

Cited by 20 publications

(25 citation statements)

References 80 publications

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“…In the antennae of both males and females, SlitGR2 and SlitGR3 are the most highly expressed GRs; this expression pattern is consistent with a previous report showing their expression in the antennae of males and females [27]. In light of these results and a recent report on Spodoptera exigua , comparing expression levels of putative CO 2 receptors in antennae, proboscis and labial palps [58], further work is required to define the molecular mechanisms and functional role of CO2 detection in S. littoralis , and indeed, other Lepidoptera.…”

Section: Discussionsupporting

confidence: 90%

Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae)

et al. 2019

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Background Deciphering the molecular mechanisms mediating the chemical senses, taste, and smell has been of vital importance for understanding the nature of how insects interact with their chemical environment. Several gene families are implicated in the uptake, recognition, and termination of chemical signaling, including binding proteins, chemosensory receptors and degrading enzymes. The cotton leafworm, Spodoptera littoralis , is a phytophagous pest and current focal species for insect chemical ecology and neuroethology. Results We produced male and female Illumina-based transcriptomes from chemosensory and non-chemosensory tissues of S. littoralis, including the antennae, proboscis, brain and body carcass. We have annotated 306 gene transcripts from eight gene families with known chemosensory function, including 114 novel candidate genes. Odorant receptors responsive to floral compounds are expressed in the proboscis and may play a role in guiding proboscis probing behavior. In both males and females, expression of gene transcripts with known chemosensory function, including odorant receptors and pheromone-binding proteins, has been observed in brain tissue, suggesting internal, non-sensory function for these genes. Conclusions A well-curated set of annotated gene transcripts with putative chemosensory function is provided. This will serve as a resource for future chemosensory and transcriptomic studies in S. littoralis and closely related species. Collectively, our results expand current understanding of the expression patterns of genes with putative chemosensory function in insect sensory and non-sensory tissues. When coupled with functional data, such as the deorphanization of odorant receptors, the gene expression data can facilitate hypothesis generation, serving as a substrate for future studies. Electronic supplementary material The online version of this article (10.1186/s12864-019-5815-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae)

et al. 2019

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“…Based on antennal transcriptome sequencing, we identified 24 IRs and 6 iGluRs in P. saucia. The putative IR co-receptors (PsauIR8a, PsauIR25a, and PsauIR76b) displayed higher expression than other IRs, which was consistent with other studies (Du et al, 2018;Walker et al, 2019;Zhao et al, 2019). According to the phylogenetic tree, six putative PsauiGluRs clustered with D. melanogaster and H. armigera iGluRs.…”

Section: Discussionsupporting

confidence: 89%

Identification of Candidate Chemosensory Receptors in the Antennae of the Variegated Cutworm, Peridroma saucia Hübner, Based on a Transcriptome Analysis

Sun

Dong

et al. 2020

Front. Physiol.

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Insect chemoreception, including olfaction and gustation, involves several families of genes, including odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs). The variegated cutworm Peridroma saucia Hübner (Lepidoptera: Noctuidae) is a worldwide agricultural pest that causes serious damage to many crops. To identify such olfactory and gustatory receptors in P. saucia, we performed a systematic analysis of the antennal transcriptome of adult P. saucia through Illumina sequencing. A total of 103 candidate chemosensory receptor genes were identified, including 63 putative ORs, 10 GRs, 24 IRs, and 6 ionotropic glutamate receptors (iGluRs). Phylogenetic relationships of these genes with those from other species were predicted, and specific chemosensory receptor genes were analyzed, including ORco, pheromone receptors (PRs), sugar receptors, CO 2 receptors, and IR coreceptors. RT-qPCR analyses of these annotated genes revealed that 6 PRs were predominantly expressed in male antennae; 3 ORs, 1 GR, 2 IRs, and 2 iGluRs had higher expression levels in male than in female antennae; and 14 ORs, 1 GR, and 3 IRs had higher expression levels in female than in male antennae. This research increases the understanding of olfactory and gustatory systems in the antennae of P. saucia and facilitates the discovery of novel strategies for controlling this pest.

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“…It is possible that some IR genes are not expressed in antennae or, alternatively, that the number of IRs is species specific and is dependent on natural habitats. Similar to Orco, both IR8a and IR25a are predicted to act as co-receptors present in the IR group because they were co-expressed along with other IRs ( Benton et al, 2009 ; Sheng et al, 2017 ; Du et al, 2018 ). TheiGluRs are a highly conserved family of ligand-gated ion channels that mediate chemical communication between neurons at synapses ( Croset et al, 2010 ; Abuin et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

“…In the process by which chemical signals are converted into electrical signals at the peripheral nerve level, both receptor and non-receptor gene families are involved. The former includes odorant receptors (ORs), ionotropic receptors (IRs), and gustatory receptors (GRs), whereas the latter includes at least odorant-binding proteins (OBPs), chemosensory proteins (CSPs), and sensory neuron membrane proteins (SNMPs) (Dunipace et al, 2001;Dahanukar et al, 2005;Jin et al, 2008;Vogt et al, 2009;Koh et al, 2014;Wicher, 2015;Agnihotri et al, 2016;Sun et al, 2016;Du et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification of Candidate Olfactory Genes in the Antennal Transcriptome of the Stink Bug Halyomorpha halys

et al. 2020

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The brown marmorated stink bug, Halyomorpha halys (Hemiptera: Pentatomidae), is a serious agricultural and urban pest that has become an invasive species in many parts of the world. Olfaction plays an indispensable role in regulating insect behaviors, such as host plant location, partners searching, and avoidance of predators. In this study, we sequenced and analyzed the antennal transcriptomes of both male and female adults of H. halys to better understand the olfactory mechanisms in this species. A total of 241 candidate chemosensory genes were identified, including 138 odorant receptors (ORs), 24 ionotropic receptors (IRs), 15 gustatory receptors (GRs), 44 odorant-binding proteins (OBPs), 17 chemosensory proteins (CSPs), and three sensory neuron membrane proteins (SNMPs). The results of semi-quantitative reverse transcription PCR (RT-PCR) assays showed that some HhalOBP and HhalCSP genes have tissue-specific and sex-biased expression patterns. Our results provide an insight into the molecular mechanisms of the olfactory system in H. halys and identify potential novel targets for pest control strategies.

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Identification and characterization of chemosensory genes in the antennal transcriptome of Spodoptera exigua

Cited by 20 publications

References 80 publications

Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae)

Transcriptome Analysis of Gene Families Involved in Chemosensory Function in Spodoptera littoralis (Lepidoptera: Noctuidae)

Identification of Candidate Chemosensory Receptors in the Antennae of the Variegated Cutworm, Peridroma saucia Hübner, Based on a Transcriptome Analysis

Identification of Candidate Olfactory Genes in the Antennal Transcriptome of the Stink Bug Halyomorpha halys

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