Identification of Candidate Olfactory Genes in the Antennal Transcriptome of the Stink Bug Halyomorpha halys

Sun, Dongdong; Huang, Yuan; Qin, Zhenjie; Zhan, Hao; Zhang, Jinping; Liu, Yang; Yang, Shiyong

doi:10.3389/fphys.2020.00876

Cited by 23 publications

(16 citation statements)

References 83 publications

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“…We referred to a bioinformatics pipeline [32,33] to identify and annotate the members of the ORs in Hemiptera. We used the protein sequences of the known gene family in insects, downloaded from the National Center for Biotechnology Information (NCBI) as a database [12,23,25,[34][35][36][37][38][39][40], and the database was used to construct a hidden Markov Model (HMM) profile for the family. The genome sequence, proteome sequence, and general feature format (GFF) annotation file of each identified hemipteran species were used as input files.…”

Section: Identification and Annotation Of Odorant Receptor Familymentioning

confidence: 99%

Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects

Tian

Dewer

et al. 2022

Insects

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Olfaction is a critical physiologic process for insects to interact with the environment, especially plant-emitted volatiles, during which odorant receptors (ORs) play an essential role in host recognition. Although OR gene evolution has been studied in many insect orders, a comprehensive evolutionary analysis and expression of OR gene gain and loss events among diverse hemipteran species are still required. In this study, we identified and analyzed 887 OR genes from 11 hemipteran species. The number of OR genes discovered in each species ranged from less than ten to hundreds. Phylogenetic analysis revealed that all identified Hemiptera OR genes were classified into seven major clades. Gene gain and loss events of OR have occurred in several species. Then, by positive selection, we discovered the amino acid differences between species to understand the molecular evolution of OR in the order Hemiptera. Additionally, we discussed how evolutionary analysis can aid the study of insect–plant communication. This study lays a foundation for subsequent investigations into the molecular mechanisms of Hemiptera olfactory receptors involved in host recognition.

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Section: Identification and Annotation Of Odorant Receptor Familymentioning

confidence: 99%

Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects

Tian

Dewer

et al. 2022

Insects

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“…It also functions as a repellent allomone to T. japonicus that significantly extends the host searching time of the parasitoid in the laboratory bioassays (Zhong et al, 2017). This alarm pheromone component was sensed by olfactory reception through H. halys OBP25, OBP30, OBP16, OBP8, and OBP4 in BMSB (Zhong et al, 2018;Sun et al, 2020). Interestingly, our results showed that TjapOBP3 was distantly related to H. halys OBP8.…”

Section: Discussionmentioning

confidence: 65%

Olfactory Reception of Host Alarm Pheromone Component by the Odorant-Binding Proteins in the Samurai Wasp, Trissolcus japonicus (Hymenoptera: Scelionidae)

et al. 2020

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The samurai wasp, Trissolcus japonicus, is the predominant egg parasitoid of the brown marmorated stink bug, Halyomorpha halys, in its native range in China. (E)-2-Decenal is a major component of the alarm pheromone of H. halys, an important invasive insect pest with significant economic importance. T. japonicus can be strongly repelled by (E)-2-decenal, and thus its host location efficiency would be reduced in the field. Better understanding on the molecular basis of olfactory reception of this host alarm pheromone component by T. japonicus may provide opportunities to develop novel approaches to enhance biological control efficacy of the parasitoid against H. halys. We identified six Odorant Binding Proteins (OBPs) from T. japonicus by transcriptome sequencing, within which three classical OBPs were expressed in a heterologous expression system with E. coli, harvested, and then challenged with (E)-2-decenal in binding assay experiments. TjapOBP2 showed the highest binding ability to (E)-2decenal, compared to TjapOBP1 and TjapOBP3. Our results unambiguously suggest that TjapOBP2 would play an important role in discriminating (E)-2-decenal and could be a possible target for further intervention in the parasitoid-host system.

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“…This study show that T. papillosa and H. halys had the highest copies of unigenes. H. halys is a polyphagous pest native to East Asia ( Sparks et al, 2020 ; Sun D. D. et al, 2020 ). Unlike T. papillosa , H. halys exhibits a wide host range.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis for Identification of Genes Related to Growth and Development, Digestion and Detoxification, Olfaction in the Litchi Stink Bug Tessaratoma papillosa

et al. 2022

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Tessaratoma papillosa is a major pest of Litchi chinensis and Dimocarpus longan. Adult and nymph secretions are not only harmful to plants but also to humans. At present, there are not a lot of research on T. papillosa, especially omics research. We used high-throughput sequencing technology to sequence the T. papillosa transcriptome and obtained 67,597 unigenes homologous to Halyomorpha halys (88.03%). Subsequently, RNA-SEQ and comparative analyses were performed on the 14 different developmental stages and tissues. A total of 462 unigenes related to growth and development, 1,851 unigenes related to digestion and detoxification, and 70 unigenes related to olfaction were obtained. Moreover, expression analysis showed that the T. papillosa major life activities genes are uniformly expressed across all developmental states. However, the adult midgut gene expression patterns were utterly different from that of the nymphs. Similarly, female fat body genes exhibited distinct expression patterns compared to that of males and nymphs. Thus, different developmental stages and physiological functions affect gene expression patterns. We also found that most of the differential genes were associated with cellular maintenance. This study will help understand the growth and development of litchi stink bugs, their choice of host plants, food digestion and detoxification, and their reproductive behavior. In addition, this result can provide reference information for some target genes in the process of control of T. papillosa.

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Identification of Candidate Olfactory Genes in the Antennal Transcriptome of the Stink Bug Halyomorpha halys

Cited by 23 publications

References 83 publications

Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects

Diversity and Molecular Evolution of Odorant Receptor in Hemipteran Insects

Olfactory Reception of Host Alarm Pheromone Component by the Odorant-Binding Proteins in the Samurai Wasp, Trissolcus japonicus (Hymenoptera: Scelionidae)

Transcriptome Analysis for Identification of Genes Related to Growth and Development, Digestion and Detoxification, Olfaction in the Litchi Stink Bug Tessaratoma papillosa

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