Identification and Characterization of Carnitine Palmitoyltransferase 1 (cpt 1) Genes in Nile Tilapia, Oreochromis niloticus

Abstract

Four cpt 1 genes ( cpt 1α1a, cpt 1α2a, cpt 1α2b, and cpt 1β) were identified in the Nile tilapia genome. Two transmembrane helix domains (TMH) were identified for Cpt 1α1a, Cpt 1α2a, and Cpt 1β, while Cpt 1α2b had only one TMH domain. Evidence was found of conserved gene synteny between cpt 1 genes from Nile tilapia and the cpt 1/CPT 1 genes of zebrafish and human. Phylogenetic analysis showed that Nile tilapia Cpt 1 sequences clustered in distinct clades with their orthologous Cpt 1/CPT 1 from other vertebrates. Nile tilapia cpt 1α1a, cpt 1α2a, cpt 1α2b, and cpt 1β contain 18 coding exons encoding polypeptides of 771, 784, 788, and 786 amino acids in length, respectively. The cpt 1 genes were determined in all the tested tissues with varying tissue distribution patterns. These findings suggest that (1) cpt 1α1a, cpt 1α2a, and cpt 1α2b arose in the Nile tilapia genome as a result of the teleost-specific whole-genome duplication; (2) nonfunctionalization is the most likely cause of the loss of cpt 1α1b in the Nile tilapia genome; (3) the different tissue-specific transcription of cpt 1α2a and cpt 1α2b may be either due to the sub- or the neo-functionalization of transcriptional control side.

Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

“…CPT1 has three isoforms, CPT1A, CPT1B and CPT1C, which control the entry of LC‐PUFAs into the mitochondria. Of these isoforms, CPT1B (CPT1β) plays a pivotal role in β‐oxidation (Bayır et al., 2020; Kerner & Hoppel, 2000), and its mRNA expression is partly controlled by PPARα, in particular (Price et al., 2000). In mammals, the PPAR response element is found in CPT1 (Morash et al., 2009).…”

Identification, characterization and nutritional regulation of fatty acid‐binding protein ( fabp ) genes by vegetable oils in European seabass ( Dicentrarchus labrax ) reared in low water temperatures

“…The tissue‐specific distribution of fabp genes in D. labrax was analysed by RT‐qPCR to gain insight into the evolution of fabp genes (Bayır et al, 2020). mRNA expression patterns of sister duplicates ( fabp7a/fabp7b , fabp10a / fabp10b and fabp11a / fabp11b ) in various tissues were significantly different.…”

Molecular cloning and characterization of three carnitine palmitoyltransferase (cpt) isoforms from mud crab (Scylla paramamosain) and their roles in respond to fasting and ambient salinity stress

“…The cpt-1a was mainly distributed in liver, kidney and colon, the cpt-1b was highly expressed in skeletal muscle, heart, and testicles (McGarry and Brown et al, 1997), while the cpt-1c was only observed in neurons (Price et al, 2002). By contrast, cpt-1 in teleosts only existed two subtypes, named cpt-1a and cpt-1b, which were found in Monopterus albus (Chao et al, 2024), Takifugu obscurus (Liu Q. et al, 2020), Pelteobagrus fulvidraco (Zheng et al, 2013), Larimichthys crocea (Wang et al, 2019), Ctenopharyngodon idellus (Shi et al, 2017), Oreochromis niloticus (Bayır et al, 2020), Sparus aurata (Boukouvala et al, 2010) and Oncorhynchus mykiss (Gutières et al, 2003). Besides, studies in C. idellus (Shi et al, 2017), O. niloticus (Bayır et al, 2020) and P. fulvidraco (Zheng et al, 2013) observed that their cpt-1a subtypes possessed multiple isoforms, which may be the results of the multiplication of fish genomes.…”

“…By contrast, cpt-1 in teleosts only existed two subtypes, named cpt-1a and cpt-1b, which were found in Monopterus albus (Chao et al, 2024), Takifugu obscurus (Liu Q. et al, 2020), Pelteobagrus fulvidraco (Zheng et al, 2013), Larimichthys crocea (Wang et al, 2019), Ctenopharyngodon idellus (Shi et al, 2017), Oreochromis niloticus (Bayır et al, 2020), Sparus aurata (Boukouvala et al, 2010) and Oncorhynchus mykiss (Gutières et al, 2003). Besides, studies in C. idellus (Shi et al, 2017), O. niloticus (Bayır et al, 2020) and P. fulvidraco (Zheng et al, 2013) observed that their cpt-1a subtypes possessed multiple isoforms, which may be the results of the multiplication of fish genomes. The cpt-1 of most fish was primarily high expression in tissues with high-energy requirements like liver, heart and muscle (Zheng et al, 2013;Shi et al, 2017;Wang et al, 2019;Bayır et al, 2020;Chao et al, 2024).…”

“…Besides, studies in C. idellus (Shi et al, 2017), O. niloticus (Bayır et al, 2020) and P. fulvidraco (Zheng et al, 2013) observed that their cpt-1a subtypes possessed multiple isoforms, which may be the results of the multiplication of fish genomes. The cpt-1 of most fish was primarily high expression in tissues with high-energy requirements like liver, heart and muscle (Zheng et al, 2013;Shi et al, 2017;Wang et al, 2019;Bayır et al, 2020;Chao et al, 2024). Compared with the cpt-1, the cpt-2 did not observe the presence of subtypes and there are few studies on cpt-2 in teleosts, which was merely covered in C. idellus (Shi et al, 2017) and M. albus (Chao et al, 2024).…”