Identification and characterization of Arabidopsis thaliana mitochondrial F1F0-ATPase inhibitor factor 1

Chen, Cuiting; Meng, Yiqing; Shopan, Jannat; Whelan, James; Hu, Zhongyuan; Yang, Jinghua; Zhang, Mingfang

doi:10.1016/j.jplph.2020.153264

Cited by 8 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By comparing the DEGs from both studies, we found only 16 genes in common. Glyma.01G227900 and Glyma.05G013800 encode steroleosin and oleosin, respectively; Glyma.15G105900, Glyma.19G028800, Glyma.20G111000, and Glyma.13G010100 encode a glucose-6-phosphate/phosphate translocator 2 (GPT2), a biotin carboxyl carrier protein (BCCP), a fatty acid desaturase (FAD2-1B), and a long chain acyl-CoA synthesis (LACS8), respectively; Glyma.15G105100 encode an aluminum-induced protein (AILP1); Glyma.20G110900 (an ortholog of AT5G04750) encodes a mitochondrial F1F0-ATP synthase inhibitor factor 1 that has been recently proposed to be crucial for plant growth and responses to abscisic acid (ABA) in A. thaliana (Chen et al, 2020); Glyma.08G064400 encodes a protein of unknown function. We also found TFs from the following families: bZIP (Glyma.02G058800 and Glyma.10G071700), NF-YA (Glyma.02G303800), C3H (Glyma.06G290100), GRF (Glyma.07G038400), B3 (Glyma.08G357600), and TALE (Glyma.17G132600).…”

Section: Resultsmentioning

confidence: 99%

“…Glyma.20G110900 (an ortholog of AT5G04750) encodes a mitochondrial F1F0-ATP synthase inhibitor factor 1 that has been recently proposed to be crucial for plant growth and responses to abscisic acid (ABA) in A. thaliana (Chen et al, 2020); Glyma.08G064400 encodes a protein of unknown function. We also found TFs from the following families: bZIP (Glyma.02G058800…”

Section: Rna-seq Analyses and The Identification Of Promising Candidate Genes For Oil Accumulation And Qualitymentioning

confidence: 99%

See 1 more Smart Citation

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Turquetti-Moraes

Moharana

Almeida‐Silva

et al. 2021

Preprint

View full text Add to dashboard Cite

Soybean is one of the major sources of edible protein and oil. Oil content is a quantitative trait that is significantly determined by genetic and environmental factors. Over the past 30 years, a large volume of soybean genetic, genomic, and transcriptomic data have been accumulated. Nevertheless, integrative analyses of such data remain scarce, in spite of their importance for crop improvement. We hypothesized that the co-occurrence of genomic regions for oil-related traits in different studies may reveal more stable regions encompassing important genetic determinants of oil content and quality in soybean. We integrated publicly available data, obtained with distinct techniques, to discover and prioritize candidate genes involved in oil biosynthesis and regulation in soybean. We detected key fatty acid biosynthesis genes (e.g., BCCP and ACCase, FADs, KAS family proteins) and several transcripton factors, which are likely regulators of oil biosynthesis. In addition, we identified new candidates for seed oil accumulation and quality, such as Glyma.03G213300 and Glyma.19G160700, which encode a translocator protein and a histone acetyltransferase, respectively. Further, oil and protein genomic hotspots are strongly associated with breeding and not with domestication, suggesting that soybean domestication prioritized other traits. The genes identified here are promising targets for breeding programs and for the development of soybean lines with increased oil content and quality.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Rna-seq Analyses and The Identification Of Promising Candidate Genes For Oil Accumulation And Qualitymentioning

confidence: 99%

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Turquetti-Moraes

Moharana

Almeida‐Silva

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…In plants, likely IF1 homologs were described in potato and rice (Norling et al, 1990;Polgreen et al, 1995;Nakazono et al, 2000). Recent bioinformatic and functional analyses further supported the IF1 candidates identified in plant mitochondria as orthologues of the IF1 proteins of yeast and animal mitochondria (Chen et al, 2020).…”

Section: Introductionmentioning

confidence: 85%

Expression of the cyanobacterial F₀F₁ ATP synthase inhibitor AtpΘ depends on small basic DNA-binding proteins and differential mRNA stability

Song

Hagemann

Becher

et al. 2021

Preprint

View full text Add to dashboard Cite

F0F1 ATP synthases produce ATP, the universal biological energy source. ATP synthase complexes on cyanobacterial thylakoid membranes use proton gradients generated either by photosynthesis or respiration. AtpΘ is an ATP synthase regulator in cyanobacteria which is encoded by the gene atpT. AtpΘ inhibits the hydrolysis of ATP (reverse reaction) that otherwise would occur under unfavorable conditions. In the cyanobacterium Synechocystis sp. PCC 6803, AtpΘ is expressed maximum in darkness but at very low levels under optimum phototrophic growth conditions or in the presence of glucose. DNA coimmunoprecipitation experiments followed by mass spectrometry identified the binding of the two transcriptional regulators cyAbrB1 and cyAbrB2 to the promoter and the histone-like protein HU to the 5’UTR of atpT. Analyses of nucleotide substitutions in the promoter and GFP reporter assays identified a functionally relevant sequence motif resembling the HLR1 element bound by the RpaB transcription factor. Electrophoretic mobility shift assays confirmed interaction of cyAbrB1, cyAbrB2 and RpaB with the promoter DNA. However, overall the effect of transcriptional regulation was comparatively low. In contrast, atpT transcript stabilities differed dramatically, half-lives were 1.6 min in the light, 33 min in the dark and substantial changes were observed if glucose or DCMU were added. These findings show that basic transcriptional control of atpT involves nucleoid-associated DNA-binding proteins, positive regulation through RpaB, while the major effect on the condition-dependent regulation of atpT expression is mediated by controlling mRNA stability, which is related to the cellular redox and energy status.IMPORTANCEF0F1 ATP synthases are protein complexes that produce ATP, the universal biological energy source in all kinds of organisms. Under unfavorable conditions, ATP synthases can operate in a futile reverse reaction, pumping protons while ATP is used up. Cyanobacteria perform plant-like photosynthesis but they cannot use the same mechanism as plants that inhibit chloroplast ATP synthases entirely during the night because respiratory and photosynthetic complexes are both located in the same membrane system. AtpΘ is a small peptide inhibitor of the reverse ATPase function in cyanobacteria encoded by the gene atpT. The production of AtpΘ is highly regulated to ensure that it is only synthetized when it is needed. In the here presented work we found that three transcription factors contribute to the regulation of atpT expression. However, we identified the control of mRNA stability as the major regulatory process governing atpT expression. Thus, it is the interplay between transcriptional and posttranscriptional regulation that position the AtpΘ-based inhibitory mechanism within the context of the cellular redox and energy balance.

show abstract

“…In plants, IF1 was first isolated in potato (Solanum tuberosum; [8,9]), and subsequently, the sequence and subcellular localization of IF1 have been analyzed in rice (Oryza sativa; [10]). In a previous study, we characterized IF1 function in Arabidopsis thaliana and established that Arabidopsis thaliana IF1 (AtIF1) not only affects the energy status of cells but also plays important roles in Plants 2021, 10, 2385 2 of 13 growth and reproductive development, as mutation of this gene results in a decrease in dark-enhanced hypocotyl elongation and seed yield [11]. Although the findings of these studies have advanced our understanding of the physiological functions of IF1 in plants, we are far from clearly elucidating the mechanisms underlying these processes.…”

Section: Introductionmentioning

confidence: 99%

“…Given that pollen formation is a highly energy-consuming process [15], mitochondrial dysfunction in pollen grains was found to drastically affect pollen development [16]. Thus, it is not surprising that reduced fertility has previously been detected in AtIF1 mutant lines [11]. Currently, little is known regarding the mechanisms underlying the activity of IF1 in plant reproductive development.…”

Section: Introductionmentioning

confidence: 99%

Identification of New Proteins and Potential Mitochondrial F1F0-ATPase Inhibitor Factor 1-Associated Mechanisms in Arabidopsis thaliana Using iTRAQ-Based Quantitative Proteomic Analysis

Chen

Meng

et al. 2021

Plants

Self Cite

View full text Add to dashboard Cite

The mitochondrial synthesis of ATP makes a vital contribution to the growth and development of biological organisms, in which the enzyme mitochondrial F1F0-ATP synthase plays a pivotal role, in that it can either synthesize or hydrolyze cellular ATP. The finding of our previous study revealed that mitochondrial F1F0-ATPase inhibitor factor 1 (IF1) in Arabidopsis thaliana has a conserved function as an endogenous inhibitor affecting cellular energy status and plays an important role in plant growth and reproduction, particularly in fertility. In this study, to gain an insight into IF1-related traits, we performed isobaric tags for relative and absolute quantitation labeling analysis. In total, 67 of 4778 identified proteins were identified as differentially expressed proteins (DEPs; 59 up-regulated and 8 down-regulated) between wild-type and if1 mutant Arabidopsis thaliana seedlings. Gene ontology enrichment analysis revealed that these DEPs were the most significantly enriched in pathways such as “long-day photoperiodism, flowering,” “positive regulation of protein import into chloroplast stroma,” and “pollen sperm cell differentiation,” which are closely associated with reproductive development. Moreover, Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that photosynthesis was the pathway most significantly enriched with DEPs. Collectively, our results revealed a global shift in protein abundance patterns corresponding to AtIF1 mutation, entailing changes in the abundance of multiple key proteins and metabolic processes, which will provide a valuable proteomic foundation for future studies.

show abstract

Identification and characterization of Arabidopsis thaliana mitochondrial F1F0-ATPase inhibitor factor 1

Cited by 8 publications

References 34 publications

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Expression of the cyanobacterial F₀F₁ ATP synthase inhibitor AtpΘ depends on small basic DNA-binding proteins and differential mRNA stability

Identification of New Proteins and Potential Mitochondrial F1F0-ATPase Inhibitor Factor 1-Associated Mechanisms in Arabidopsis thaliana Using iTRAQ-Based Quantitative Proteomic Analysis

Contact Info

Product

Resources

About

Identification and characterization of Arabidopsis thaliana mitochondrial F1F0-ATPase inhibitor factor 1

Cited by 8 publications

References 34 publications

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Integrating omics approaches to discover and prioritize candidate genes involved in oil biosynthesis in soybean

Expression of the cyanobacterial F0F1 ATP synthase inhibitor AtpΘ depends on small basic DNA-binding proteins and differential mRNA stability

Identification of New Proteins and Potential Mitochondrial F1F0-ATPase Inhibitor Factor 1-Associated Mechanisms in Arabidopsis thaliana Using iTRAQ-Based Quantitative Proteomic Analysis

Contact Info

Product

Resources

About

Expression of the cyanobacterial F₀F₁ ATP synthase inhibitor AtpΘ depends on small basic DNA-binding proteins and differential mRNA stability